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A molecularly imprinted polymer-based electrochemical sensor for the determination of tofacitinib

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Abstract

Tofacitinib citrate (TOF) is a Janus kinase-3 inhibitor used for rheumatoid arthritis treatment. In this study, a molecularly imprinted polymer (MIP)–based sensor was produced using acrylamide as the functional monomer via photopolymerization technique for the electrochemical determination of TOF. This study is the first one to explain the electrochemical determination of TOF with a highly selective MIP-based sensor. The surface characterization of the MIP-based sensor was performed with scanning electron microscopy and energy-dispersive X-ray spectroscopy methods, and it was expanded with electrochemical characterization by cyclic voltammetry and electrochemical impedance spectroscopy (EIS) methods. TOF determination was performed using differential pulse voltammetry (DPV) and EIS methods in standard solution and spiked serum sample in the linear range between 1×10−11 M and 1×10−10 M. Very low limit of detection and limit of quantification values were found, confirming the sensitivity of the sensor. Recovery analysis with spiked serum and tablet samples verified the sensor’s accuracy and applicability using DPV and EIS methods. The selectivity of the sensor was confirmed with imprinting factor and interference studies, and the sensor performance was controlled using non-imprinted polymer for comparison at every step.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

Fatma Budak thanks the financial support from the Council of Higher Education 100/2000 (YOK) under the special 100/2000 scholarship program and TUBITAK under the ARDEB/1004 Ph.D. Scholarship Programs. Ahmet Cetinkaya thanks the financial support from the Council of Higher Education 100/2000 (YOK) under the special 100/2000 scholarship program and the Scientific and Technological Research Council of Turkey (TUBITAK) under the BIDEB/2211-A Ph.D. and ARDEB/1004 Ph.D. Scholarship Programs.

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Authors and Affiliations

  1. Faculty of Pharmacy, Department of Analytical Chemistry, Ankara University, Ankara, Türkiye

    Fatma Budak, Ahmet Cetinkaya & Sibel A. Ozkan

  2. Graduate School of Health Sciences, Ankara University, Ankara, Türkiye

    Fatma Budak & Ahmet Cetinkaya

  3. Gulhane Faculty of Pharmacy, Department of Analytical Chemistry, University of Health Sciences, Ankara, Türkiye

    S. Irem Kaya

  4. DEVA Holding A.S., R&D Center, Tekirdag, Türkiye

    Esen Bellur Atici

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  1. Fatma Budak
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  2. Ahmet Cetinkaya
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  3. S. Irem Kaya
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  4. Esen Bellur Atici
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  5. Sibel A. Ozkan
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Correspondence to S. Irem Kaya or Sibel A. Ozkan.

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Budak, F., Cetinkaya, A., Kaya, S.I. et al. A molecularly imprinted polymer-based electrochemical sensor for the determination of tofacitinib. Microchim Acta 190, 205 (2023). https://doi.org/10.1007/s00604-023-05790-3

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  • DOI: https://doi.org/10.1007/s00604-023-05790-3

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