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Immunofluorescent-aggregation assay based on anti-Salmonella typhimurium IgG-AuNCs, for rapid detection of Salmonella typhimurium

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Abstract

Sensitive and rapid detection of pathogenic bacteria plays an important role in avoiding food poisoning. However, the practical application value of conventional assays for detection of foodborne bacteria, are limited by major drawbacks; these include the laboriousness of pure culture preparation, complexity of DNA extraction for polymerase chain reaction, and low sensitivity of enzyme-linked immunosorbent assay. Herein, we designed a non-complex strategy for the sensitive, quantitative, and rapid detection of Salmonella typhimurium with high specificity, using an anti-Salmonella typhimurium IgG-AuNC-based immunofluorescent-aggregation assay. Salmonella typhimurium was agglutinated with fluorescent anti-Salmonella typhimurium IgG-AuNC on a glass slide, and observed using a fluorescence microscope with photoexcitation and photoemission at 560 nm and 620 nm, respectively. Under optimized reaction conditions, the AuNC-based immunofluorescent-aggregation assay had a determination range between 7.0 × 103 and 3.0 × 108 CFU/mL, a limit of detection of 1.0 × 103 CFU/mL and an assay response time of 3 min. The technique delivered good results in assessing real samples.

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Funding

This study received financial support from the National Natural Science Foundation of China (21675024, 21804021), the Joint Funds for the Innovation of Science and Technology, Fujian Province (2016Y9056), the Program for Innovative Leading Talents in Fujian Province (2016B016), the Science and Technology Project of Quanzhou (2018N122S), and the Medical Elite Cultivation Program of Fujian (2020GGB049).

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Authors and Affiliations

  1. Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, School of Pharmacy, Fujian Medical University, Fuzhou, 350004, China

    Quan-Quan Zhuang, Shao-Bin He, Kai-Yuan Huang, Ying-Ying Xu, Hua-Ping Peng, Hao-Hua Deng & Wei Chen

  2. Department of Pharmacy, Affiliated Quanzhou First Hospital of Fujian Medical University, Quanzhou, 362000, China

    Quan-Quan Zhuang

  3. Department of Laboratory Medicine, Affiliated Quanzhou First Hospital of Fujian Medical University, Quanzhou, 362000, China

    Yan-Cheng Jiang

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  1. Quan-Quan Zhuang
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  2. Shao-Bin He
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Correspondence to Hao-Hua Deng or Wei Chen.

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Zhuang, QQ., He, SB., Jiang, YC. et al. Immunofluorescent-aggregation assay based on anti-Salmonella typhimurium IgG-AuNCs, for rapid detection of Salmonella typhimurium. Microchim Acta 189, 160 (2022). https://doi.org/10.1007/s00604-022-05263-z

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