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A multifunctional nanozyme-based enhanced system for tert-butyl hydroquinone assay by surface-enhanced Raman scattering

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Abstract

An Au-based nanozyme composite (AuNPs/Cu,I) was constructed by using Cu,I-doped carbon dots (Cu,I-CDs) as the reducing agent as well as the nanozyme. Notably, AuNPs/Cu,I nanozyme not only possessed the intrinsic activity of mimicking enzymes of superoxide dismutase, peroxidase, and catalase at different conditions but was also employed as surface-enhanced Raman spectroscopy (SERS) enhancer. The combination of Cu,I-CDs and AuNPs promoted the electron transferability, leading to increased peroxidase-like activity and superoxide-like activity. Compared to the individual Cu,I-CDs and AuNPs nanozyme, the AuNPs/Cu,I composite demonstrated promising peroxidase-like activity by transferring electrons instead of generating OH. Interestingly, the multienzyme-like activity of AuNPs/Cu,I nanozyme could be finely tuned by changing the composition of Cu0/Cu+ and Au. The tert-butyl hydroquinone (TBHQ) as the substrate could be catalyzed with AuNPs/Cu,I nanozyme to produce red substances, resulting in a significant Raman enhancement effect at the same time, showing good linear range from 0.11 to 10 mg L−1. Overall, the current investigation provides a flexible and controllable way to design multifunctional nanozymes along with the Raman enhancement strategy based on the catalysis of nanozyme.

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Funding

This work was greatly supported by the Analysis and Testing Foundation of Kunming University of Science and Technology (grant numbers 2019P20173118001) and Research on the Key Technology of Vegetable Deep Processing (grant numbers 2019ZG001-4–2). Yang Dezhi gratefully acknowledges financial support from Kunming University of Science and Technology high-level talent research platform construction funding (grant numbers 20200097) and 2019 Kunming University of Science and Technology Graduate Research and International Exchange Project Fund and Yunnan Lunyang Technology Co., Ltd.

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Authors and Affiliations

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan Province, China

    Dezhi Yang, Qiulan Li, Yijie Wang, Hong Li & Yaling Yang

  2. Yunnan Lunyang Technology Co., Ltd., Kunming, 650032, Yunnan Province, China

    Dezhi Yang & Qian Zhang

  3. Institute of Agro-products Processing, Yunnan Academy of Agricultural Sciences, Kunming, 650032, Yunnan Province, China

    Hong Li

  4. School of Physics, University of Hyderabad, Hyderabad, Telangana, 500046, India

    Sai Kumar Tammina

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  1. Dezhi Yang
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Yang, D., Li, Q., Zhang, Q. et al. A multifunctional nanozyme-based enhanced system for tert-butyl hydroquinone assay by surface-enhanced Raman scattering. Microchim Acta 189, 29 (2022). https://doi.org/10.1007/s00604-021-05135-y

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