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Construction of an electrochemical sensor with graphene aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin

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Abstract

A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor. The ZrO2/CS/rGOA composite was prepared by one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate, and subsequently be freeze-dried. Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA. Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (−0.8–0.8 V). The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups. Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.. Finally, the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction was used for the detection of luteolin in real samples. Recoveries of  spiked samples with different concentrations were in the range 78.6–103.3% with a relative RSD lower than 12.0%.

Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode. The electrode was employed for the detection of luteolin coupled with the solid-phase extraction technique.

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References

  1. Rygula A, Wrobel TP, Szklarzewicz J, Baranska M (2013) Raman and UV–vis spectroscopy studies on luteolin–Al(III) complexes. Vib Spectrosc 64:21–26

    Article  CAS  Google Scholar 

  2. Bajoub A, Pacchiarotta T, Hurtado-Fernández E, Olmo-García L, García-Villalba R, Fernández-Gutiérrez A, Mayboroda OA, Carrasco-Pancorbo A (2016) Comparing two metabolic profiling approaches (liquid chromatography and gas chromatography coupled to mass spectrometry) for extra-virgin olive oil phenolic compounds analysis a botanical classification perspective. J Chromatogr A 1428:267–279

    Article  CAS  Google Scholar 

  3. Wu T, Yu C, Li R, Li J (2018) Minireview: recent advances in the determination of flavonoids by capillary electrophoresis. Instrum Sci Technol 46:364–386

    Article  CAS  Google Scholar 

  4. Xu JJ, An M, Yang R, Cao J, Ye LH, Peng LQ (2016) Trace amounts of poly-beta-cyclodextrin wrapped carbon nanotubes for the microextraction of flavonoids in honey samples by capillary electrophoresis with light-emitting diode induced fluorescence detection. Electrophoresis 37:1891–1901

    Article  CAS  Google Scholar 

  5. Cao M, Yin X, Bo X, Guo L (2018) High-performance electrocatalyst based on metal-organic framework macroporous carbon composite for efficient detection of luteolin. J Electroanal Chem 824:153–160

    Article  CAS  Google Scholar 

  6. Feng X, Yin X, Bo X, Guo L (2019) An ultrasensitive luteolin sensor based on MOFs derived CuCo coated nitrogen-doped porous carbon polyhedron. Sensor Actuat B-Chem 281:730–738

    Article  CAS  Google Scholar 

  7. Hayasaka N, Shimizu N, Komoda T, Mohri S, Tsushida T, Eitsuka T, Miyazawa T, Nakagawa K (2018) Absorption and metabolism of luteolin in rats and humans in relation to in vitro anti-inflammatory effects. J Agric Food Chem 66:11320–11329

    Article  CAS  Google Scholar 

  8. Cai Y, Huang W, Wu K (2020) Morphology-controlled electrochemical sensing of erbium- benzenetricarboxylic acid frameworks for azo dyes and flavonoids. Sensor Actuat B-Chem 304:127370–127378

    Article  CAS  Google Scholar 

  9. Gao F, Tu X, Ma X, Xie Y, Zou J, Huang X, Qu F, Yu Y, Lu L (2020) NiO@Ni-MOF nanoarrays modified Ti mesh as ultrasensitive electrochemical sensing platform for luteolin detection. Talanta 215:120891

    Article  CAS  Google Scholar 

  10. Xie Y, Zhang T, Chen Y, Wang Y, Wang L (2020) Fabrication of core-shell magnetic covalent organic frameworks composites and their application for highly sensitive detection of luteolin. Talanta 213:120843

    Article  CAS  Google Scholar 

  11. Anik U, Timur S, Dursun Z (2019) Metal organic frameworks in electrochemical and optical sensing platforms: a review. Microchim Acta 186:196

    Article  Google Scholar 

  12. Asadian E, Ghalkhani M, Shahrokhian S (2019) Electrochemical sensing based on carbon nanoparticles: a review. Sensor Actuat B-Chem 293:183–209

    Article  CAS  Google Scholar 

  13. Liu X, Long L, Yang W, Chen L, Jia J (2018) Facilely electrodeposited coral-like copper micro−/nano-structure arrays with excellent performance in glucose sensing. Sensor Actuat B-Chem 266:853–860

    Article  CAS  Google Scholar 

  14. Vogiazi V, de la Cruz A, Mishra S, Shanov V, Heineman WR, Dionysiou DD (2019) A comprehensive review: development of electrochemical biosensors for detection of cyanotoxins in freshwater. ACS Sens 4:1151–1173

    Article  CAS  Google Scholar 

  15. Wang Y, Zeng Z, Qiao J, Dong S, Liang Q, Shao S (2021) Ultrasensitive determination of nitrite based on electrochemical platform of AuNPs deposited on PDDA-modified MXene nanosheets. Talanta 221:121605–121612

    Article  CAS  Google Scholar 

  16. Krishnan SK, Singh E, Singh P, Meyyappan M, Nalwa HS (2019) A review on graphene-based nanocomposites for electrochemical and fluorescent biosensors. RSC Adv 9:8778–8881

    Article  CAS  Google Scholar 

  17. Nag A, Mitra A, Mukhopadhyay SC (2018) Graphene and its sensor-based applications: a review. Sensor Actuat B-Phys 270:177–194

    Article  CAS  Google Scholar 

  18. Baig N, Saleh TA (2018) Electrodes modified with 3D graphene composites: a review on methods for preparation, properties and sensing applications. Microchim Acta 185:283

    Article  Google Scholar 

  19. Idowu A, Boesl B, Agarwal A (2018) 3D graphene foam-reinforced polymer composites – a review. Carbon 135:52–71

    Article  CAS  Google Scholar 

  20. Lu L (2018) Recent advances in synthesis of three-dimensional porous graphene and its applications in construction of electrochemical (bio)sensors for small biomolecules detection. Biosens Bioelectron 110:180–192

    Article  CAS  Google Scholar 

  21. Baranwal A, Kumar A, Priyadharshini A, Oggu GS, Bhatnagar I, Srivastava A, Chandra P (2018) Chitosan: An undisputed bio-fabrication material for tissue engineering and bio-sensing applications. Int J Biol Macromol 110:110–123

    Article  CAS  Google Scholar 

  22. Hu X, Goud KY, Kumar VS, Catanante G, Li Z, Zhu Z, Marty JL (2018) Disposable electrochemical aptasensor based on carbon nanotubes- V2O5-chitosan nanocomposite for detection of ciprofloxacin. Sensor Actuat B-Chem 268:278–286

    Article  CAS  Google Scholar 

  23. Li Y, Liu Y, Kim E, Song Y, Tsao CY, Teng Z, Gao T, Mei L, Bentley WE, Payne GF, Wang Q (2018) Electrodeposition of a magnetic and redox-active chitosan film for capturing and sensing metabolic active bacteria. Carbohydr Polym 195:505–514

    Article  CAS  Google Scholar 

  24. Wu Z, Guo F, Huang L, Wang L (2018) Electrochemical nonenzymatic sensor based on cetyltrimethylammonium bromide and chitosan functionalized carbon nanotube modified glassy carbon electrode for the determination of hydroxymethanesulfinate in the presence of sulfite in foods. Food Chem 259:213–218

    Article  CAS  Google Scholar 

  25. George JM, Antony A, Mathew B (2018) Metal oxide nanoparticles in electrochemical sensing and biosensing: a review. Microchim Acta 185:358

    Article  Google Scholar 

  26. Liu A, Kou W, Zhang H, Xu J, Zhu L, Kuang S, Huang K, Chen H, Jia Q (2020) Quantification of trace organophosphorus pesticides in environmental water via enrichment by magnetic-zirconia nanocomposites and online extractive electrospray ionization mass spectrometry. Anal Chem 92:4137–4145

    Article  CAS  Google Scholar 

  27. Zhang QR, Bolisetty S, Cao YP, Handschin S, Adamcik J, Peng QM, Mezzenga R (2019) Selective and efficient removal of fluoride from water: in situ engineered amyloid fibril/ZrO2 hybrid membranes. Angew Chem Int Edit 58:6012–6016

    Article  CAS  Google Scholar 

  28. Reddy CV, Reddy IN, Harish VVN, Reddy KR, Shetti NP, Shim J, Aminabhavi TM (2020) Efficient removal of toxic organic dyes and photoelectrochemical properties of iron-doped zirconia nanoparticles Chemosphere:239

  29. Reddy CV, Reddy IN, Ravindranadh K, Reddy KR, Shetti NP, Kim D, Shim J, Aminabhavi TM (2020) Copper-doped ZrO2 nanoparticles as high-performance catalysts for efficient removal of toxic organic pollutants and stable solar water oxidation. J environ manage 260:

  30. Reddy CV, Reddy IN, Reddy KR, Jaesool S, Yoo K (2019) Template-free synthesis of tetragonal co-doped ZrO2 nanoparticles for applications in electrochemical energy storage and water treatment. Electrochim Acta 317:416–426

    Article  CAS  Google Scholar 

  31. Hou XD, Tang S, Guo XX, Wang LC, Liu X, Lu XF, Guo Y (2018) Preparation and application of guanidyl-functionalized graphene oxide-grafted silica for efficient extraction of acidic herbicides by box-Behnken design. J Chromatogr A 1571:65–75

    Article  CAS  Google Scholar 

  32. Hou X, Liu S, Zhou P, Li J, Liu X, Wang L, Guo Y (2016) Polymeric ionic liquid modified graphene oxide-grafted silica for solid-phase extraction to analyze the excretion-dynamics of flavonoids in urine by box-Behnken statistical design. J Chromatogr A 1456:10–18

    Article  CAS  Google Scholar 

  33. Zeng L, Zhang Y, Wang H, Guo L (2013) Electrochemical behavior of luteolin and its detection based on macroporous carbon modified glassy carbon electrode. Anal Methods 5:3365

    Article  CAS  Google Scholar 

  34. Pang P, Liu Y, Zhang Y, Gao Y, Hu Q (2014) Electrochemical determination of luteolin in peanut hulls using graphene and hydroxyapatite nanocomposite modified electrode. Sensor Actuat B-Chem 194:397–403

    Article  CAS  Google Scholar 

  35. Ma Y, Kong Y, Xu J, Deng Y, Lu M, Yu R, Yuan M, Li T, Wang J (2020) Carboxyl hydrogel particle film as a local pH buffer for voltammetric determination of luteolin and baicalein. Talanta 208:120373

    Article  CAS  Google Scholar 

  36. Tesio AY, Granero AM, Vettorazzi NR, Ferreyra NF, Rivas GA, Fernández H, Zon MA (2014) Development of an electrochemical sensor for the determination of the flavonoid luteolin in peanut hull samples. Microchem J 115:100–105

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31901766, 81703228), Financial support from the Talents of High Level Scientific Research Foundation, Qingdao Agricultural University (No.1119014, 1120023), and Breeding Plan of Shandong Provincial Qingchuang Research Team (2019).

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Authors and Affiliations

  1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao Shandong Province, 266109, China

    Xiudan Hou, Wei Wu, Fangyuan Zhao & Qingli Yang

  2. College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Shandong Qingdao, 266042, China

    Wancui Xie

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  1. Xiudan Hou
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  3. Fangyuan Zhao
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Correspondence to Qingli Yang.

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Hou, X., Wu, W., Zhao, F. et al. Construction of an electrochemical sensor with graphene aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin. Microchim Acta 188, 86 (2021). https://doi.org/10.1007/s00604-021-04743-y

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