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Carbon nanotube–mediated antibody-free suspension array for determination of typical endocrine-disrupting chemicals

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Abstract

A carbon nanotube (CNT)–mediated antibody-free suspension array (CASA) by integration of functionalized CNTs and aptamer (Apt) into xMAP® technology for simultaneous determination of typical endocrine-disrupting chemicals (EDCs) was developed . The interaction between CNTs and Apt acts as an effective and straightforward signal recognition, transformation, and amplification strategy. The amino-functionalized CNTs are covalently modified on the carboxyl-functionalized magnetic bead (MB) and further physically bridging with biotinylated Apt. CNTs on the surface of MBs not only increase the amount of Apt for target binding and signal amplification but also maintain the biological activity of Apt. After magnetic separation, the encoded MB address was distinguished and the concentration of the target in the liquid was negatively correlated with median fluorescence intensity. A series of environmental water samples were analysed by CASA, traditional immuno-SA, and competitive inhibition enzyme-linked immunosorbent assay for validation. The results obtained using CASA well matched for the multiplexed detection of various targets with dynamic concentration range from 6.40 × 10−5 to 4.00 μg L−1 within 1 h. The method also confirmed good selectively, accuracy, and consistency with high-performance liquid chromatography.

Schematic representation of amino-functionalized carbon nanotube (CNT)–bridged antibody-free suspension array for detecting of three typical endocrine-disrupting chemicals. The amino-functionalized CNTs are covalently modified and further physically interacted with biotinylated aptamer featuring in the recognition and binding with the target of interest.

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Funding

The authors gratefully acknowledge the financial support by the National Natural Science Foundation of China (No. 81872584, 81273078, 81472941, 81671784, and 21505027), the National 863 Young Scientist Program (No. 2015AA020940), the Beijing Nova Program (Z181100006218017), the Natural Science Foundation of Tianjin City (No. 16JCZDJC39500), the Science and Technology Major Project of Tianjin City (No. 16ZXHLSY00130), the Natural Science Foundation of Guangdong Province (2016A030313138), and the Key Projects of Guangzhou Science and Technology Program (No. 201704020056).

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Authors and Affiliations

  1. School of Public Health, Guangzhou Medical University, Guangzhou, 511436, People’s Republic of China

    Nan Liu, Weijie Liang, Ya Li & Lugang Deng

  2. CAS Center for Excellence in Nanoscience, CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, People’s Republic of China

    Jing Wu & Yunlei Xianyu

  3. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Yiping Chen

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  1. Nan Liu
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Correspondence to Nan Liu, Yunlei Xianyu or Yiping Chen.

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Liu, N., Wu, J., Xianyu, Y. et al. Carbon nanotube–mediated antibody-free suspension array for determination of typical endocrine-disrupting chemicals. Microchim Acta 187, 202 (2020). https://doi.org/10.1007/s00604-020-4181-3

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