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Surface-enhanced Raman spectroscopy in tandem with a gradient electric field from 4-mercaptophenylboronic acid on silver nanoparticles

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Abstract

The surface-enhanced Raman spectroscopy (SERS) signal of a reporter on silver nanoparticles can be effectively gained by gradient electric field application. The external electric field initiates the dielectrophoresis of nanoparticles and their electrically induced dipole–dipole interaction. Owing to dielectrophoresis, the nanoparticles are concentrated in the area of high electrical field strength. The induced dipole–dipole interaction leads to additional coagulation of nanoparticles and formation of hotspots. Both dielectrophoresis and induced dipole–dipole interaction increase the number of hotspots, which leads to a SERS signal growth. These two mechanisms of SERS signal amplification are explained by the dielectrophoresis and Derjaguin–Landau–Verwey–Overbeek theories. The benefits of the surface-enhanced Raman spectroscopy in tandem with the gradient electric field are experimentally confirmed using a SERS-active reporter, 4-mercaptophenylboronic acid which has a characteristic peak at Raman shift of 1586 cm−1, conjugated to silver nanoparticles of 32, 52, 58, and 74 nm in diameter. The SERS signal gain depends on the silver nanoparticle stability, size, and electric field strength. The limit of detection for 4-mPBA in the system under study can be calculated from the concentration plot and equals to 63 nM. The enhancement factor calculated for SERS in tandem with the gradient electric field can reach 106.

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Data availability

The data that support the findings of this study are available from the corresponding author, Sorokina O.N., via e-mail: alsiona@gmail.com, upon reasonable request.

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Funding

This work was performed under the Russian Federation State Assignment no АААА-А19-119071890024-8 and AAAA-A19-119110790066-5.

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Authors and Affiliations

  1. N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russian Federation, 119334

    Sergey N. Podoynitsyn, Olga N. Sorokina, Natalia L. Nechaeva, Sergey V. Yanovich & Ilya N. Kurochkin

  2. Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russian Federation, 119234

    Ilya N. Kurochkin

Authors
  1. Sergey N. Podoynitsyn
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  2. Olga N. Sorokina
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  3. Natalia L. Nechaeva
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  4. Sergey V. Yanovich
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  5. Ilya N. Kurochkin
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Contributions

S.N. Podoynitsyn—theoretical discussion; O.N. Sorokina, N.L. Nechaeva—experimental setup and results discussion; S. V. Yanovich—computer modelling; I. N. Kurochkin—research management and consulting

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Correspondence to Olga N. Sorokina.

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Podoynitsyn, S.N., Sorokina, O.N., Nechaeva, N.L. et al. Surface-enhanced Raman spectroscopy in tandem with a gradient electric field from 4-mercaptophenylboronic acid on silver nanoparticles. Microchim Acta 187, 566 (2020). https://doi.org/10.1007/s00604-020-04550-x

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