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Colorimetric detection of Staphylococcus aureus using gold nanorods labeled with yolk immunoglobulin and urease, magnetic beads, and a phenolphthalein impregnated test paper

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Abstract

A colorimetric test is described for the rapid detection of Staphylococcus aureus (SA). Gold nanorods (AuNRs) are first labeled with urease and yolk immunoglobulin (IgY). This probe can specifically bind SA. In the next step, nonspecific magnetic beads and sample are added. This leads to the formation of the AuNR-IgY-SA-nMB immunocomplex which is then magnetically separated. Finally, a solution of urea is added to the supernatant. Ureases catalyzes the decomposition of urea which results in an increase in the pH value. The increase in the pH value is detected by using a phenolphthalein test paper which undergoes a color change from white to pink. The analytical process can be completed within 20 min. The method is highly specific and can detect as little as 476 cfu·mL−1. It was verified by analyzing contaminated Chinese cabbage and beef samples, and 1000 cfu·mL−1 of SA were accurately detected.

Schematic representation of a colorimetric method for the detection of Staphylococcus aureus based on the immunocomplex formed from dual-labeled gold nanorod (AuNR) probe, bacteria and non-specific magnetic bead (nMB). This method can be completed within 20 min.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No.: 81872668, 81473018), Jilin Province Science and Technology Development Plan Item (Grant No.: 20170204003SF) and Graduate Innovation Fund of Jilin University (Grant No.: 101832018C061).

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Author notes

  1. Bo Pang and Yan Zheng contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, Jilin University, Changchun, 130021, People’s Republic of China

    Bo Pang, Juan Wang, Yushen Liu, Xiuling Song, Jinhua Li, Shuo Yao, Kaiyue Fu, Kun Xu, Chao Zhao & Juan Li

  2. Department of Geriatrics, the First Hospital of Jilin University, Changchun, 130021, People’s Republic of China

    Yan Zheng

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  1. Bo Pang
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  2. Yan Zheng
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Correspondence to Kun Xu, Chao Zhao or Juan Li.

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Pang, B., Zheng, Y., Wang, J. et al. Colorimetric detection of Staphylococcus aureus using gold nanorods labeled with yolk immunoglobulin and urease, magnetic beads, and a phenolphthalein impregnated test paper. Microchim Acta 186, 611 (2019). https://doi.org/10.1007/s00604-019-3722-0

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