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RETRACTED ARTICLE: A SERS-based lateral flow assay for the stroke biomarker S100-β

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This article was retracted on 18 January 2021

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Abstract

A surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) is described for the quantitative analysis of the proteinic stroke biomarker S100-β that has to be detected at very low concentration levels. The Raman reporter 5,5′-dithiobis-2-nitrobenzoic acid (DTNB) on gold nanoparticles (GNPs) was employed as the SERS tags. They are shown to perform much better than bare GNPs in LF strips. The S100-β protein can be detected by this method with very low detection limits by monitoring the intensity of the characteristic Raman peak of the S100-β protein-conjugated GNPs at 1332 cm−1. Under optimized conditions, the assay works in the 1 pg·mL−1 to 40 ng·mL−1 S100-β concentration range, and the detection limit is as low as 0.14 pg·mL−1. This is lower by a factor of 3 compared to colorimetric or fluorimetric methods.

Schematic illustration of the configuration (A) and the principle of the SERS-based lateral flow assay for quantification of S100-β (B).

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Acknowledgements

This work was supported in part by funds from the National Natural Science Foundation of China (Grant No., 81471212, 81701179, 81501106 and 81271275), Taishan Scholars Project of Shandong Province, Development Plan of Science and Technology of Traditional Chinese Medicine in Shandong (Grant No.2017-245); the Natural Science Foundation of Shandong (Grant No. ZR2012HZ006).

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Authors and Affiliations

  1. Key Lab of Cerebral Microcirculation in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271000, Shandong, China

    Ying Wang, Yajun Hou, Hanxia Li, Mingfeng Yang & Baoliang Sun

  2. Radiology College, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271000, Shandong, China

    Peng Zhao

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  1. Ying Wang
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  2. Yajun Hou
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Correspondence to Peng Zhao or Baoliang Sun.

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Wang, Y., Hou, Y., Li, H. et al. RETRACTED ARTICLE: A SERS-based lateral flow assay for the stroke biomarker S100-β. Microchim Acta 186, 548 (2019). https://doi.org/10.1007/s00604-019-3634-z

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