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MnO2 nanosheets as oxidase mimics for colorimetric detection of alkaline phosphatase activity

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Abstract

A sensitive colorimetric method is described for the determination of the activity of alkaline phosphatase (ALP). It is based on the regulation of the oxidase-mimicking activity of MnO2 nanosheets. In the absence of ALP, MnO2 nanosheets are capable of catalyzing the oxidation of the colorless substrate 3,3′,5,5′-tetramethylbenzidine (TMB) by oxygen to form a blue oxidized product (TMB Ox) with an absorption peak at 652 nm. In the presence of ALP and its substrate ascorbic acid-2-phosphate, the latter is hydrolyzed to form ascorbic acid (AA). AA triggers the decomposition of MnO2 nanosheets by reducing MnO2 to Mn2+, thereby weakening the enzyme mimicking activity of the MnO2 nanosheets and causing a drop in absorbance. The drop in absorbance at 652 nm is related to the ALP activity in the range from 0.05–10 m-units per mL (mU·mL−1), and the detection limit is 0.05 mU·mL−1. The method was applied to the determination of ALP in spiked calf serum samples and gave satisfactory results.

Schematic presentation of a facile and sensitive colorimetric method for detecting the activity of alkaline phosphatase (ALP) based on enzymatic regulation of the oxidase-mimicking activity of MnO2 nanosheets.

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Acknowledgements

The authors thank the National Natural Science Foundation of China (No. 21405125), the Natural Science Foundation Project of CQ (No. cstc2018jscx-msybX0263) and the Fundamental Research Funds for the Central Universities (No. XDJK2019C026) for kindly support. In addition, the authors also thank China Agriculture Research System (No. CARS-27), National Risk Assessment Program for Agricultural Products Quality and Safety (No. GJFP2019003 and GJFP2019013) for financial support.

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Authors and Affiliations

  1. Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture, Citrus Research Institute, Southwest University, Chongqing, 400712, People’s Republic of China

    Fengyu Tian, Jing Zhou, Bining Jiao & Yue He

  2. National Citrus Engineering Research Center, Chongqing, People’s Republic of China

    Fengyu Tian, Jing Zhou, Bining Jiao & Yue He

  3. College of Food Science, Southwest University, Chongqing, 400712, People’s Republic of China

    Fengyu Tian, Jing Zhou, Jing Ma, Siyuan Liu, Bining Jiao & Yue He

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  1. Fengyu Tian
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  2. Jing Zhou
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  4. Siyuan Liu
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Correspondence to Yue He.

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All procedures performed in studies involving animals were in accordance with the ethical standards of Chinese laws and guidelines (GKFCZ2001545).

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Tian, F., Zhou, J., Ma, J. et al. MnO2 nanosheets as oxidase mimics for colorimetric detection of alkaline phosphatase activity. Microchim Acta 186, 408 (2019). https://doi.org/10.1007/s00604-019-3519-1

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