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3D nitrogen-doped graphite foam@Prussian blue: an electrochemical sensing platform for highly sensitive determination of H2O2 and glucose

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Abstract

A platform is described for voltammetric sensing of hydrogen peroxide (H2O2). It is based on the use of nitrogen-doped graphite foam modified with Prussian Blue particles (PB/NGF). Graphite foam was synthesized by chemical vapor deposition, and doping with nitrogen was realized via dielectric barrier plasma discharge. PB particles were grown on the NGF through electrodeposition. SEM images of NGF verified the porous and interconnected structure of graphite foam, and XPS and Raman spectroscopy verified the successful doping with N. The performance of the PB/NGF electrode was characterized by CV and EIS which showed it to possess outstanding properties in terms of sensing H2O2. H2O2 was quantified in a range of 0.004 to 1.6 mM with a detection limit of 2.4 μM. The PB/NGF electrode also is shown to be a viable substrate for loading glucose oxidase (GOx). The GOx-functionalized electrode responds to glucose over the 0.2 to 20 mM concentration range at a potential of −50 mV (vs. Ag/AgCl), with a sensitivity of 27.48 mA M−1 cm−2 and a 0.1 M detection limit (at an S/N ratio of 3). The glucose sensor is selective, stable, and reproducible. The biosensor was successfully applied to the determination of glucose in spiked human serum samples, and this confirmed it practicability.

Schematic of a self-supporting amperometric glucose biosensor based on glucose oxidase and Prussian blue modified 3D nitrogen doped graphite foam.

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Acknowledgments

The project financially supported by National Natural Science Foundation of China (81773680, 81460543).

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Authors and Affiliations

  1. Key Laboratory of Xinjiang Plant Resources and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832000, China

    Yu Zhang & Bintong Huang

  2. Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, 832003, China

    Feng Yu

  3. School of Materials Science and Engineering, Harbin Institute of Technology, Shenzhen, 518055, China

    Qunhui Yuan

  4. Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China

    Meng Gu

  5. Collage of Chemical Engineering, Sichuan University, Chengdu, 610000, China

    Junyi Ji

  6. Shenzhen Key Laboratory of Organic Pollution Prevention and Control, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, 518055, China

    Yang Zhang

  7. College of Science, Harbin Institute of Technology, Shenzhen, 518055, China

    Yang Zhang & Yingchun Li

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  1. Yu Zhang
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  2. Bintong Huang
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  3. Feng Yu
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  7. Yang Zhang
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Correspondence to Yang Zhang or Yingchun Li.

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Zhang, Y., Huang, B., Yu, F. et al. 3D nitrogen-doped graphite foam@Prussian blue: an electrochemical sensing platform for highly sensitive determination of H2O2 and glucose. Microchim Acta 185, 86 (2018). https://doi.org/10.1007/s00604-017-2631-3

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  • DOI: https://doi.org/10.1007/s00604-017-2631-3

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