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Voltammetric determination of tumor necrosis factor-α based on the use of an aptamer and magnetic nanoparticles loaded with gold nanoparticles

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Abstract

The authors are presenting an electrochemical aptasensor for tumor necrosis factor-alpha (TNF-α) detection that is aided by the use of magnetic nanoparticles (NPs) and two DNA probes. Firstly, magnetic NPs coated with gold NPs (Fe3O4@AuNP) are synthesized. Then, DNA probe 1 with a terminal thiol group is immobilized on the surface of Fe3O4@AuNP via gold-thiol chemistry. DNA probe 1 is then hybridized with DNA probe 2, which is labeled with Methylene Blue (MB). The composite of Fe3O4@AuNP-DNA duplex is formed as a result, which can be easily absorbed on a magnetized glassy carbon electrode. The electrochemical signal is obtained after reductant-mediated amplification. Since MB-labeled DNA probe 2 is the aptamer against TNF-α, it will be released in the presence of TNF-α. This process leaves Fe3O4@AuNP-DNA probe 1 on the surface of the electrode. Thus, the recorded electrochemical response decreases dramatically. The assay, best operated at a working voltage of −0.39 V (vs. SCE), has a linear response in the 10 pg mL−1 to 100 ng·mL−1 TNF-α concentration range, and the limit of detection is 10 pg mL−1. The sensing strategy is highly sensitive and selective, and has been successfully applied to real samples.

Schematic of the biosensor. The composite of Fe3O4@AuNP-DNA duplex is placed on a glassy carbon electrode. After interaction between TNF-α and its aptamer, DNA probe is released with the labeled electrochemical species. The decrease in electrochemical response serves as the analytical signal.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant no. 31400847) and the Science and Technology Program of Suzhou (Grant no. SYG201605).

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Authors and Affiliations

  1. CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, People’s Republic of China

    Peng Miao, Dawei Yang, Xifeng Chen, Zhenzhen Guo & Yuguo Tang

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  1. Peng Miao
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  2. Dawei Yang
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  3. Xifeng Chen
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  4. Zhenzhen Guo
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  5. Yuguo Tang
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Correspondence to Peng Miao.

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Miao, P., Yang, D., Chen, X. et al. Voltammetric determination of tumor necrosis factor-α based on the use of an aptamer and magnetic nanoparticles loaded with gold nanoparticles. Microchim Acta 184, 3901–3907 (2017). https://doi.org/10.1007/s00604-017-2419-5

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  • DOI: https://doi.org/10.1007/s00604-017-2419-5

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