Abstract
The authors describe the preparation of Fe2(MoO4)3 materials with micrometer dimensions, and show that they are highly efficient peroxidase mimics that can be used in a photometric assay for H2O2. Depending on the experimental conditions of synthesis, the Fe2(MoO4)3 micromaterials display different morphologies, surface properties, and peroxidase-like activities. Among them, Fe2(MoO4)3 with flower-like structure has the largest BET specific surface area and the most negative potential. It exhibits the best peroxidase mimetic activity in catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of H2O2 to generate a blue-green product. Based on these findings, method was worked out for H2O2 determination that allows both visual and photometric read-out. The assay has a detection limit as low as 0.7 μM, is selective and practical. Given its sensitivity, the method has a good potential with respect to determination of H2O2 in clinical samples but also in oxidase-type of reactions where H2O2 is being produced.
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References
Xiang ZB, Wang Y, Ju P, Zhang D (2016) Optical determination of hydrogen peroxide by exploiting the peroxidase-like activity of AgVO3 nanobelts. Microchim Acta 183(1):457–463
Zhang TB, YC L, Luo GS (2014) Synthesis of hierarchical iron hydrogen phosphate crystal as a robust peroxidase mimic for stable H2O2 detection. ACS Appl Mater Interfaces 6(16):14433–14438
Chang Q, Deng KJ, Zhu LH, Jiang GD, Yu C, Tang HQ (2009) Determination of hydrogen peroxide with the aid of peroxidase-like Fe3O4 magnetic nanoparticles as the catalyst. Microchim Acta 165(3–4):299–305
Liu ZH, Cai RX, Mao LY, Huang HP, Ma WH (1999) Highly sensitive spectrofluorimetric determination of hydrogen peroxide with β-cyclodextrin-hemin as catalyst. Analyst 124(2):173–176
Khorshid A, Amin RR, Issa YM (2013) Fabrication of a novel highly selective and sensitive nano-molar Cu(I), Cu(II) membrane sensors based on thiosemicarbazide and acetaldehydethiosemi-carbazone copper complexes. J Chem Acta 1(1):52–58
Chen X, Zhou XD, JM H (2012) Pt-DNA complexes as peroxidase mimetics and their applications in colorimetric detection of H2O2 and glucose. Anal Methods 4(7):2183–2187
Gao LZ, Zhuang J, Nie L, Zhang JB, Zhang Y, Gu N, Wang TH, Feng J, Yang DL, Perrett S, Yan X (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2(9):577–583
Qiao FM, Chen LJ, Li XN, Li LF, Ai SY (2014) Peroxidase-like activity of manganese selenide nanoparticles and its analytical application for visual detection of hydrogen peroxide and glucose. Sensors Actuators B 193(3):255–262
Song YJ, KG Q, Zhao C, Ren JS, XG Q (2010) Graphene oxide: intrinsic peroxidase catalytic activity and its application to glucose detection. Adv Mater 22(19):2206–2210
Chang Q, Tang HQ (2014) Optical determination of glucose and hydrogen peroxide using a nanocomposite prepared from glucose oxidase and magnetite nanoparticles immobilized on graphene oxide. Microchim Acta 181(5–6):527–534
Pérez-López B, Arben M (2012) Carbon nanotubes and graphene in analytical sciences. Microchim Acta 179(1–2):1–16
Ju P, Xiang YH, Xiang ZB, Wang M, Zhao Y, Zhang D, JQ Y, Han XX (2016) BiOI hierarchical nanoflowers as novel robust peroxidase mimetics for colorimetric detection of H2O2. RSC Adv 6(21):17483–17493
Chen LJ, Sun B, Wang XD, Qiao FM, Ai SY (2013) 2D ultrathin nanosheets of Co-Al layered double hydroxides prepared in L-asparagine solution: enhanced peroxidase-like activity and colorimetric detection of glucose. J Mater Chem B 1(17):2268–2274
Wang W, Jiang XP, Chen KZ (2012) CePO4: Tb, Gd hollow nanospheres as peroxidase mimic and magnetic-fluorescent imaging agent. Chem Commun 48(54):6839–6841
Qiao FM, Wang Z, Xu K, Ai SY (2015) Double enzymatic cascade reactions within FeSe-Pt@SiO2 nanospheres: Synthesis and application toward colorimetric biosensing of H2O2 and glucose. Analyst 140(19):6684–6691
YZ Y, Ju P, Zhang D, Han XX, Yin XF, Zheng L, Sun CJ (2016) Peroxidase-like activity of FeVO4 nanobelts and its analytical application for optical detection of hydrogen peroxide. Sensors Actuators B 233:162–172
Ding CP, Yan YH, Xiang DS, Zhang CL, Xian YZ (2016) Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay. Microchim Acta 183(2):625–631
Wang N, Sun JC, Chen LJ, Fan H, Ai SY (2015) A Cu2(OH)3Cl-CeO2 nanocomposite with peroxidase-like activity, and its application to the determination of hydrogen peroxide, glucose and cholesterol. Microchim Acta 182(9):1733–1738
Čunderlová V, Hlaváček A, Horňáková V, Peterek M, Němeček D, Hampl A, Eyer L, Skládal P (2016) Catalytic nanocrystalline coordination polymers as an efficient peroxidase mimic for labeling and optical immunoassays. Microchim Acta 183(2):651–658
Zhang Y, Lu F, Yan ZQ, Wu D, Ma HM, Du B, Wei Q (2015) Electrochemiluminescence immunosensing strategy based on the use of Au@Ag nanorods as a peroxidase mimic and NH4CoPO4 as a supercapacitive supporter: application to the determination of carcinoembryonic antigen. Microchim Acta 182:1421–1429
Liang G, Liu XH (2015) G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic. Microchim Acta 182(13):2233–2240
Hayat A, Haider W, Raza Y, Marty JL (2015) Colorimetric cholesterol sensor based on peroxidase like activity of zinc oxide nanoparticles incorporated carbon nanotubes. Talanta 143:157–161
JS M, Wang Y, Zhao M, Zhang L (2012) Intrinsic peroxidase-like activity and catalase-like activity of Co3O4 nanoparticles. Chem Commun 48(19):2540–2542
Lin YH, Ren JS, XG Q (2014) Catalytically active nanomaterials: a promising candidate for artificial enzymes. Acc Chem Res 47(4):1097–1105
Li D, Xue JQ, Liu MB (2015) Synthesis of Fe2(MoO4)3 microspheres by self-assembly and photocatalytic performances. New J Chem 39:1910–1915
Zhang ZY, Li WY, Ng TW, Kang WP, Lee CS, Zhang WJ (2015) Iron(II) molybdate (FeMoO4) nanorods as a high-performance anode for lithium ion batteries: structural and chemical evolution upon cycling. J Mater Chem A 3(41):20527–20534
Kim TH, Ramachandra B, Choi JS, Saidutta MB, Choo KY, Song SD, Rhee YW (2004) Selective oxidation of methanol to formaldehyde using modified iron-molybdate catalysts. Catal Lett 98(2):161–165
Liu HY, CC G, Li DJ, Zhang MZ (2015) Non-enzymatic hydrogen peroxide biosensor based on rose-shaped FeMoO4 nanostructures produced by convenient microwave-hydrothermal method. Mater Res Bull 64:375–379
Ye LQ, Chen JN, Tian LH, Liu JY, Peng TY, Deng KJ, Zan L (2013) BiOI thin film via chemical vapor transport: photocatalytic activity, durability, selectivity and mechanism. Appl Catal B Environ:130–131
Song YP, Wang H, Li ZH, Ye NQ, Wang LJ, Liu Y (2015) Fe2(MoO4)3 nanoparticle-anchored MoO3 nanowires: strong coupling via the reverse diffusion of heteroatoms and largely enhanced lithium storage properties. RSC Adv 5:16386–16393
Chen LJ, Wang N, Wang XD, Ai SY (2013) Protein-directed in situ synthesis of platinum nanoparticles with superior peroxidase-like activity, and their use for photometric determination of hydrogen peroxide. Microchim Acta 180(15):1517–1522
Li LL, Ai LH, Zhang CH, Jiang J (2014) Hierarchical {001}-faceted BiOBr microspheres as a novel biomimetic catalyst: dark catalysis towards colorimetric biosensing and pollutant degradation. Nanoscale 6(9):4627–4634
Nicell JA, Wright H (1997) A model of peroxidase activity with inhibition by hydrogen peroxide. Enzym Microb Technol 21(4):302–310
Chattopadhyay K, Mazumdar S (2000) Structural and conformational stability of horseradish peroxidase: effect of temperature and pH. Biochemistry 39(1):263–270
Ai LH, Li LL, Zhang CH, Fu J, Jiang J (2013) MIL-53(Fe): a metal-organic framework with intrinsic peroxidase-like catalytic activity for colorimetric biosensing. Chem-Eur J 19(19):15105–15108
Porter DJ, Bright HJ (1983) The mechanism of oxidation of nitroalkanes by horseradish peroxidase. J Biol Chem 258(16):9913–9924
Wei H, Wang EK (2008) Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection. Anal Chem 80(6):2250–2254
Lv CJ, Di WH, Liu ZH, Zheng KZ, Qin WP (2014) Luminescent CePO4: Tb colloids for H2O2 and glucose sensing. Analyst 139(18):4547–4555
Dutta AK, Maji SK, Srivastava DN, Mondal A, Biswas P, Paul P, Adhikary B (2012) Synthesis of FeS and FeSe nanoparticles from a single source precursor: a study of their photocatalytic activity, peroxidase-like behavior, and electrochemical sensing of H2O2. ACS Appl Mater Interfaces 4(4):1919–1927
Acknowledgments
This work was supported by The National Natural Science Foundation of China-Shandong Joint Funded Project (U1406403), Qingdao Talent (13-CX-20), National Science Foundation of China (41306074), “Top Hundred Talents Program” of Chinese Academy of Sciences (CAS) and the CAS-Japan Society for the Promotion of Science (JSPS) Collaborative Research Program (GJHZ1317). C.J. Sun would also like to thank support from Taishan Scholar and the Ministry of Human Resources and Social Security of China.
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Bing Wang and Peng Ju contributed equally to this work.
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Wang, B., Ju, P., Zhang, D. et al. Colorimetric detection of H2O2 using flower-like Fe2(MoO4)3 microparticles as a peroxidase mimic. Microchim Acta 183, 3025–3033 (2016). https://doi.org/10.1007/s00604-016-1955-8
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DOI: https://doi.org/10.1007/s00604-016-1955-8