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Urea-modified metal-organic framework of type MIL-101(Cr) for the preconcentration of phosphorylated peptides

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Abstract

Mass spectrometry (MS) is the most powerful tool in phosphoproteomics research. However, phosphopeptides usually are present in low concentrations and their preconcentration therefore is highly desired. We describe a two-step method for the synthesis of a metal organic framework of the type MIL-101(Cr) that is modified with urea (then designated as MIL-101(Cr)-UR2). It possesses large surface area, good solvent stability and high affinity for some phosphates. Due to the presence of modified urea functions, this material allows for selective and effective enrichment of phosphorylated peptides. It was successfully applied to the enrichment of phosphopeptides from non-fat-milk. The method was applied to the detection of phosphopeptides in a tryptic digest of β-casein where is showed a detection sensitivity as low as 10−10 M.

A two step method was designed to synthesis MIL-101(Cr)-UR2 and the prepared green solid was successfully applied to recognize phosphopeptides. (Amino functionalized metal-organic framework (MOF-NH2); urea modified metal-organic framework (MOF-UR2); matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS))

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Acknowledgments

The financial support from Tianjin Research Program of Application Foundation and Advanced Technology (15JCYBJC23800) is gratefully acknowledged.

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Authors and Affiliations

  1. Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, China

    Xiaoqing Yang & Yan Xia

  2. Key Laboratory of Biosensing and Molecular Recognition, Tianjin, 300071, China

    Yan Xia

  3. State Key Laboratory of Medicinal Chemical Biology, Tianjin, 300071, China

    Yan Xia

  4. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300071, China

    Yan Xia

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  1. Xiaoqing Yang
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  2. Yan Xia
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Correspondence to Yan Xia.

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Yang, X., Xia, Y. Urea-modified metal-organic framework of type MIL-101(Cr) for the preconcentration of phosphorylated peptides. Microchim Acta 183, 2235–2240 (2016). https://doi.org/10.1007/s00604-016-1860-1

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  • DOI: https://doi.org/10.1007/s00604-016-1860-1

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