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Calcein-functionalized Fe3O4@SiO2 nanoparticles as a reusable fluorescent nanoprobe for copper(II) ion

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Abstract

Calcein was first covalently grafted onto the surface of magnetic silica nanoparticles to obtain a novel fluorescent nanoprobe for sensitive and selective determination of Cu(II) ion. The nanoparticles were synthesized in a two-step reaction under mild conditions and characterized using small-angle X-ray scattering, Fourier transform infrared spectroscopy, transmission electron microscopy, and thermogravimetric analysis. The nanoprobe also displays good selectivity over other metal ions and is highly stable under conditions of high ionic strength. The response to Cu(II) ranges from 80 nM to 1.25 μM. The detection limit (LOD) is 43 nM which is lower than previously reported LODs. The probe can be regenerated by adding the complexing agent EDTA and also can be separated from a sample mixture by applying a magnetic field. We conclude that this method represents a new and convenient approach to the development of magnetic and reusable optical probes.

Calcein was first covalently grafted onto the surface of magnetic silica nanoparticles to obtain a novel fluorescent nanoprobe for sensitive and selective determination of Cu(II) ion. The nanoprobe displays good selectivity over other metal ions and is highly stable under conditions of high ionic strength.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21205002) and the Innovation Funds of Anhui Normal University (2014cxjj09).

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Authors and Affiliations

  1. College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo/Biosensing, The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-based Materials, Anhui Normal University, Wuhu, 241000, People’s Republic of China

    Jinshui Liu, Guoning Liu, Lingjie Zang & Wenxiu Liu

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  1. Jinshui Liu
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  2. Guoning Liu
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  3. Lingjie Zang
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  4. Wenxiu Liu
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Correspondence to Jinshui Liu.

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Liu, J., Liu, G., Zang, L. et al. Calcein-functionalized Fe3O4@SiO2 nanoparticles as a reusable fluorescent nanoprobe for copper(II) ion. Microchim Acta 182, 547–555 (2015). https://doi.org/10.1007/s00604-014-1358-7

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  • DOI: https://doi.org/10.1007/s00604-014-1358-7

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