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Nonenzymatic sensing of glucose at neutral pH values using a glassy carbon electrode modified with graphene nanosheets and Pt-Pd bimetallic nanocubes

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Abstract

We report on a nonenzymatic method for the determination of glucose using an electrode covered with graphene nanosheets (GNs) modified with Pt-Pd nanocubes (PtPdNCs). The latter were prepared on GNs by using N,N-dimethylformamide as a bifunctional solvent for the reduction of both metallic precursors and graphene oxide, and for confining the growth of PtPdNCs on the surface. The modified electrode displays strong and sensitive current response to the electrooxidation of glucose, notably at pH 7. The sensitivities increase in the order of Pt1Pd5NCs< Pt1Pd3NCs< Pt5Pd1NCs< Pt3Pd1NCs< Pt1Pd1NCs. At an applied potential of +0.25 V, the electrode responds linearly (R = 0.9987) to glucose in up to 24.5 mM concentration, with a sensitivity of 1.4 μA cm−2 M−1. The sensor is not poisoned by chloride, and not interfered by ascorbic acid, uric acid and p-acetamidophenol under normal physiological conditions. The modified electrode also displays a wide linear range, good stability and fast amperometric response, thereby indicating the potential of the bimetallic materials for nonenzymatic sensing of glucose.

nonenzymatic electrochemical method was developed for glucose determination using an electrode modified with PtPd nanocubes/graphene nanosheets (PtPdNCs/GNs). The new material shows a good performance in the sensing of glucose, thus is promising for the future development of nonenzymatic glucose sensors.

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Acknowledgments

We thank the Japan Society for the Promotion of Science (JSPS) for the fellowship. This work was financially supported by the National Natural Science Foundation of China (Nos. 21305050), the Scientific Research Foundation of Shangda Li, Jimei University, China (ZC2013005), the Science and Technology Planning Project of Fujian Province, China (2012Y0052) and JSPS KAKENHI Grant Nos. 24·02335 and 24550100.

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Authors and Affiliations

  1. College of Biological Engineering, Jimei University, Xiamen, 361021, China

    Xiaomei Chen, Xiaotian Tian, Limin Zhao & Zhiyong Huang

  2. Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto, 615-8520, Japan

    Xiaomei Chen & Munetaka Oyama

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  1. Xiaomei Chen
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  2. Xiaotian Tian
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  3. Limin Zhao
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  4. Zhiyong Huang
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Correspondence to Xiaomei Chen or Zhiyong Huang.

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Chen, X., Tian, X., Zhao, L. et al. Nonenzymatic sensing of glucose at neutral pH values using a glassy carbon electrode modified with graphene nanosheets and Pt-Pd bimetallic nanocubes. Microchim Acta 181, 783–789 (2014). https://doi.org/10.1007/s00604-013-1142-0

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  • DOI: https://doi.org/10.1007/s00604-013-1142-0

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