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The complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays

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Abstract

The replacement of antibodies by molecularly imprinted polymers (MIPs) has been investigated for many decades. However, indirect protocols (including natural primary and secondary antibodies) are still utilized to evaluate the ability of MIP thin films to recognize target molecules. MIPs can be prepared as either a thin film or as particles, and cavities that are complementary to the template can be generated on their surfaces. We have prepared thin film MIPs and particle MIPs prepared by solvent evaporation and phase inversion, respectively, from solutions of poly(ethylene-co-vinyl alcohol) (pEVAL) in the presence of the target analytes amylase, lysozyme, and lipase. These were first adsorbed on MIP thin films and by MIP particles that contain fluorescent quantum dots. Sandwich fluoroimmunoassays were then conducted to quantify them in MIP-coated 96-well microplates. The method was applied to determine amylase in saliva, and results were compared with a commercial analytical system.

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Acknowledgments

We appreciate financial supports from National Science Council of ROC under Contract No. NSC 100-2314-B-390-001-MY3 and NSC 101-2220-E-390-001-.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, I-Shou University, Kaohsiung, 84001, Taiwan

    Mei-Hwa Lee & Wei-Ti Chin

  2. Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, 87131, USA

    James L. Thomas

  3. Department of Chemical and Materials Engineering, National University of Kaohsiung (NUK), 700, Kaohsiung University Rd., Nan-Tzu District, Kaohsiung, 81148, Taiwan

    Yun-Chao Chen & Hung-Yin Lin

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  1. Mei-Hwa Lee
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  2. James L. Thomas
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  3. Yun-Chao Chen
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  4. Wei-Ti Chin
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  5. Hung-Yin Lin
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Correspondence to Hung-Yin Lin.

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Lee, MH., Thomas, J.L., Chen, YC. et al. The complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays. Microchim Acta 180, 1393–1399 (2013). https://doi.org/10.1007/s00604-013-0995-6

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  • DOI: https://doi.org/10.1007/s00604-013-0995-6

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