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FET immunosensor for hemoglobin A1c using a gold nanofilm grown by a seed-mediated technique and covered with mixed self-assembled monolayers

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Abstract

A micro FET-based immunosensor was developed for the determination of hemoglobin-A1c (HbA1c). The HbA1c/hemoglobin ratio is an important index in diabetes control. The sensor was fabricated by Complementary Metal-Oxide-Semiconductor Transistor (CMOS) and Micro Electronic Mechanical System (MEMS) techniques. The antibodies were immobilized via mixed self-assembled monolayers (SAMs) on a gold nanofilm. The nanofilm was deposited on a gold electrode by seed-mediated growth and gave a uniform and well distributed coverage. Nonspecific sites and interferences by noise were eliminated by covering the AuNPs with mixed SAMs. Compared to the immunosensor fabricated via the mixed SAMs method without gold nanofilm, the immunosensor displays a more than 2-fold sensitivity. The immunosensor is capable of detecting HbA1c and hemoglobin in hemolyzed and diluted whole blood, and results showed good agreement with the established clinical method.

Based on CMOS and MEMS techniques, a micro FET-based immunosensor was developed for the hemoglobin-A1c level determination. The antibodies were immobilized based on the mixed self-assembled monolayers and seed-mediated growth method. The immunosensor can detect HbA1c and hemoglobin simultaneously and has good potential for clinical application.

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Acknowledgment

This work is supported by the National Basic Research Program of China (973 Program) (Project Number 2009CB320300).

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Authors and Affiliations

  1. State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China

    Qiannan Xue, Chao Bian, Jianhua Tong, Jizhou Sun, Hong Zhang & Shanhong Xia

  2. Graduate University of Chinese Academic of Sciences, Beijing, 100049, People’s Republic of China

    Qiannan Xue

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  1. Qiannan Xue
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  2. Chao Bian
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  3. Jianhua Tong
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  4. Jizhou Sun
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  5. Hong Zhang
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Correspondence to Shanhong Xia.

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Xue, Q., Bian, C., Tong, J. et al. FET immunosensor for hemoglobin A1c using a gold nanofilm grown by a seed-mediated technique and covered with mixed self-assembled monolayers. Microchim Acta 176, 65–72 (2012). https://doi.org/10.1007/s00604-011-0675-3

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  • DOI: https://doi.org/10.1007/s00604-011-0675-3

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