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One-step construction of an electrode modified with electrodeposited Au/SiO2 nanoparticles, and its application to the determination of NADH and ethanol

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Abstract

A new electrode was developed by one-step potentiostatic electrodeposition (at −2.0 V for 20 s) of Au/SiO2 nanoparticles on a glassy carbon electrode. The resulting electrode (nano-Au/SiO2/GCE) was characterized by scanning electronic microscopy, X-ray photoelectron spectroscopy and electrochemical techniques. The electrochemical behavior of dihydronicotinamide adenine dinucleotide (NADH) at the nano-Au/SiO2/GCE were thoroughly investigated. Compared to the unmodified electrode, the overpotential decreased by about 300 mV, and the current response significantly increased. These changes indicated that the modified electrode showed excellent catalytic activity in the oxidation of NADH. A linear relationship was obtained in the NADH concentration range from 1.0 × 10−6 to 1.0 × 10−4 mol L−1. In addition, amperometric sensing of ethanol at the nano-Au/SiO2/GCE in combination with alcohol dehydrogenase and nicotinamide adenine dinucleotide was successfully demonstrated. A wide linear response was also found for ethanol in the range from 5.0 × 10−5 to 1.0 × 10−3 mol L−1 and 1.0 × 10−3 to 1.0 × 10−2 mol L−1, respectively. The method was successfully applied to determine ethanol in beer and biological samples.

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Acknowledgements

The authors gratefully acknowledge the financial support from the National Nature Science Foundation of China (No. 20705041), the Natural Science Foundation of Hubei Province (No. 2007ABA127), the Nature Science Foundation of the South-Central University for Nationalities (No. XTZ09005) and the Special Fund for Basic Scientific Research of Central Colleges, South-Central University for Nationalities (No. ZZZ10002).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, 430074, China

    Xiaoqing Liu, Buhai Li, Xian Wang & Chunya Li

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  1. Xiaoqing Liu
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  2. Buhai Li
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  3. Xian Wang
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  4. Chunya Li
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Corresponding author

Correspondence to Chunya Li.

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Figure S1

Influence of HAuCl4 concentration on the current response of 5.0 × 10-4 mol L-1 NADH at the nano−Au/SiO2/GCE. (DOC 121 kb)

Figure S2

Influence of electrodeposition time on the current response of 5.0 × 10-4 mol L-1 NADH at the nano−Au/SiO2/GCE. (DOC 181 kb)

Figure S3

Amperometric response of the unmodified GCE (a) and the nano−Au/SiO2/GCE (b) for the oxidation of NADH at + 0.36 V in 10 mL of phosphate buffer solution (pH 7.0) with successive injection 10 μL of 1.0 × 10-3 mol L−1 NADH. (DOC 128 kb)

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Liu, X., Li, B., Wang, X. et al. One-step construction of an electrode modified with electrodeposited Au/SiO2 nanoparticles, and its application to the determination of NADH and ethanol. Microchim Acta 171, 399–405 (2010). https://doi.org/10.1007/s00604-010-0441-y

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  • DOI: https://doi.org/10.1007/s00604-010-0441-y

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