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Rapid determination of momordicoside in Momoradica charantia seeds by capillary zone electrophoresis

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Abstract

A novel method is described for the rapid determination of momordicoside in bitter melon based on capillary zone electrophoresis. Electrophoresis conditions, including the pH value and concentration of running buffer, as well as the voltage applied were optimized. The contents of momordicoside in 52 samples originating from different agricultural areas in China were determined. The results showed an excellent linearity in the concentration range of 0.50–2.51 mg mL−1 for all samples (R 2 > 0.9999), with a recovery of 96.0% and a relative standard deviation of 1.4%. The method is characterized by rapidity, accuracy, the need for small samples only, and is suitable for analyses at a large scale.

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Acknowledgments

We thank Dr. TANG Lin from Sichuan University of China for her kind supply of momordicoside A. The study was supported by the Ph.D. Programs Foundation of Ministry of Education of China (no. 2002061088).

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Authors and Affiliations

  1. The College of Life Science, Sichuan University, Chengdu, 610064, China

    Pu Xuan, Qun Sun & Fang Chen

  2. The Institute of Biological and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China

    Pu Xuan

  3. Pharmacy College, University of Traditional Chinese Medicine in Chengdu, Chengdu, 610075, China

    Xin Chen & Jia-bei Liang

Authors
  1. Pu Xuan
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  2. Xin Chen
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  3. Qun Sun
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  4. Jia-bei Liang
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  5. Fang Chen
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Corresponding author

Correspondence to Fang Chen.

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Xuan, P., Chen, X., Sun, Q. et al. Rapid determination of momordicoside in Momoradica charantia seeds by capillary zone electrophoresis. Microchim Acta 165, 35–38 (2009). https://doi.org/10.1007/s00604-008-0093-3

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  • DOI: https://doi.org/10.1007/s00604-008-0093-3

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