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The usage of a bismuth film electrode as transducer in glucose biosensing

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Abstract.

A second generation glucose biosensor was developed by using neutral red (NR) as a mediator and a bismuth film electrode (BiFE) as a transducer along with immobilized glucose oxidase. The linear range was between 0.2 and 2.5 mM, and a correlation coefficient of 0.999 was obtained with this electrode. The standard deviation (at 1 mM glucose for n = 4) and the coefficient of variation were calculated as ±8.07 µM and 3.4%, respectively. The biosensor was used for the determination of glucose in wine samples.

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References

  • J Wang J Lu S B Hočevar P A M Farias (2000) ArticleTitleBismuth-coated carbon electrodes for anodic stripping voltammetry Anal Chem 72 3218 Occurrence Handle10.1021/ac000108x Occurrence Handle1:CAS:528:DC%2BD3cXjvVahtLw%3D

    Article  CAS  Google Scholar 

  • E A Hutton S B Hočevar B Ogorevc M R Smyth (2003) ArticleTitleBismuth film electrode for simultaneous adsorptive stripping analysis of trace cobalt and nickel using constant current chronopotentiometric and voltammetric protocol Electrochem Commun 5 765 Occurrence Handle10.1016/S1388-2481(03)00177-2 Occurrence Handle1:CAS:528:DC%2BD3sXntVKht7w%3D

    Article  CAS  Google Scholar 

  • J Wang J Lu U A Kirgoz S B Hočevar B Ogorevc (2001) ArticleTitleInsights into the anodic stripping voltammetric behavior of bismuth film electrodes Anal Chim Acta 434 29 Occurrence Handle10.1016/S0003-2670(01)00818-2 Occurrence Handle1:CAS:528:DC%2BD3MXjt12nsbk%3D

    Article  CAS  Google Scholar 

  • J Wang U Anik Kirgoz J Lu (2001) ArticleTitleStripping voltammetry with the electrode material acting as a ‘built-in’ internal Standard Electrochem Commun 3 703 Occurrence Handle10.1016/S1388-2481(01)00242-9 Occurrence Handle1:CAS:528:DC%2BD3MXptFWhsLg%3D

    Article  CAS  Google Scholar 

  • G Kefala A Economou A Voulgaropoulos M Sofoniou (2003) ArticleTitleA study of bismuth-film electrodes for the detection of trace metals by anodic stripping voltammetry and their application to the determination of Pb and Zn in tap water and human hair Talanta 6 603 Occurrence Handle10.1016/S0039-9140(03)00350-3 Occurrence Handle1:CAS:528:DC%2BD3sXos12ru7k%3D

    Article  CAS  Google Scholar 

  • A Charalambous A Economou (2005) ArticleTitleA study on the utility of bismuth-film electrodes for the determination of In(III) in the presence of Pb(II) and Cd(II) by square wave anodic stripping voltammetry Anal Chim Acta 547 53 Occurrence Handle10.1016/j.aca.2004.12.048 Occurrence Handle1:CAS:528:DC%2BD2MXntVCltbY%3D

    Article  CAS  Google Scholar 

  • A Krolicka A Bobrowski K Kalcher J Mocak I Svancara K Vytras (2003) ArticleTitleStudy on catalytic adsorptive stripping voltammetry of trace cobalt at bismuth film electrodes Electroanalysis 15 1859 Occurrence Handle10.1002/elan.200302763 Occurrence Handle1:CAS:528:DC%2BD2cXhs1Wgtw%3D%3D

    Article  CAS  Google Scholar 

  • D Demetriades A Economou A Voulgaropoulos (2004) ArticleTitleA study of pencil-lead bismuth-film electrodes for the determination of trace metals by anodic stripping voltammetry Anal Chim Acta 519 167 Occurrence Handle10.1016/j.aca.2004.05.008 Occurrence Handle1:CAS:528:DC%2BD2cXmt1Kgsbc%3D

    Article  CAS  Google Scholar 

  • S Legeai O Vittori (2006) ArticleTitleA Cu/Nafion/Bi electrode for on-site monitoring of trace heavy metals in natural waters using anodic stripping voltammetry: an alternative to mercury-based electrodes Anal Chim Acta 560 184 Occurrence Handle10.1016/j.aca.2005.12.010 Occurrence Handle1:CAS:528:DC%2BD28XhvFOhsrs%3D

    Article  CAS  Google Scholar 

  • E A Hutton S B Hočevar M Ogorevc (2005) ArticleTitleEx situ preparation of bismuth film microelectrode for use in electrochemical stripping microanalysis Anal Chim 537 285 Occurrence Handle10.1016/j.aca.2005.01.040 Occurrence Handle1:CAS:528:DC%2BD2MXjtl2ls7k%3D

    Article  CAS  Google Scholar 

  • S B Hočevar J Wang R P Deo B Ogorevc (2002) ArticleTitlePotentiometric stripping analysis at bismuth-film electrode Electroanalysis 14 112 Occurrence Handle10.1002/1521-4109(200201)14:2<112::AID-ELAN112>3.0.CO;2-5

    Article  Google Scholar 

  • E A Hutton B Ogorevc S B Hočevar F Weldon M R Smyth J Wang (2001) ArticleTitleAn introduction to bismuth film electrode for use in cathodic electrochemical detection Electrochem Commun 3 707 Occurrence Handle10.1016/S1388-2481(01)00240-5 Occurrence Handle1:CAS:528:DC%2BD3MXptFWhsLc%3D

    Article  CAS  Google Scholar 

  • G Kefala A Economou M Sofoniou (2006) ArticleTitleDetermination of trace aluminium by adsorptive stripping voltammetry on a preplated bismuth-film electrode in the presence of cupferron Talanta 68 1013 Occurrence Handle10.1016/j.talanta.2005.07.001 Occurrence Handle1:CAS:528:DC%2BD28Xhs1Squw%3D%3D

    Article  CAS  Google Scholar 

  • M Yang Z Zhang Z Hu J Li (2006) ArticleTitleDifferential pulse anodic stripping voltammetry detection of metallothionein at bismuth film electrodes Talanta 69 1162 Occurrence Handle10.1016/j.talanta.2005.12.041 Occurrence Handle1:CAS:528:DC%2BD28XlvFygt7w%3D

    Article  CAS  Google Scholar 

  • M Bučková P GrQndler G U Flechsig (2005) ArticleTitleAdsorptive stripping voltammetric detection of daunomycin at a bismuth bulk electrode Electroanalysis 17 440 Occurrence Handle10.1002/elan.200403178 Occurrence Handle1:CAS:528:DC%2BD2MXjtFajtL4%3D

    Article  CAS  Google Scholar 

  • S Legeai O Vittori (2006) ArticleTitleA Cu/Nafion/Bi electrode for on-site monitoring of trace heavy metals in natural waters using anodic stripping voltammetry: an alternative to mercury-based electrodes Anal Chim Acta 560 184 Occurrence Handle10.1016/j.aca.2005.12.010 Occurrence Handle1:CAS:528:DC%2BD28XhvFOhsrs%3D

    Article  CAS  Google Scholar 

  • E A Hutton B Ogorevc S B Hočevar M R Smyth (2006) ArticleTitleBismuth film microelectrode for direct voltammetric measurement of trace cobalt and nickel in some simulated and real body fluid samples Anal Chim Acta 557 57 Occurrence Handle10.1016/j.aca.2005.10.003 Occurrence Handle1:CAS:528:DC%2BD28XotFyjtg%3D%3D

    Article  CAS  Google Scholar 

  • E A Hutton S B Hočevar B Ogorevc (2005) ArticleTitleEx situ preparation of bismuth film microelectrode for use in electrochemical stripping microanalysis Anal Chim Acta 537 285 Occurrence Handle10.1016/j.aca.2005.01.040 Occurrence Handle1:CAS:528:DC%2BD2MXjtl2ls7k%3D

    Article  CAS  Google Scholar 

  • U Anik Kirgoz S Marin M Pumera A Merkoci S Alegret (2005) ArticleTitleStripping voltammetry with bismuth modified graphite-epoxy composite electrodes Electroanalysis 17 881 Occurrence Handle10.1002/elan.200403167 Occurrence Handle1:CAS:528:DC%2BD2MXkvFChsro%3D

    Article  CAS  Google Scholar 

  • M T Castaneda B Perez M Pumera M del Vale A Merkoci S Alegret (2005) ArticleTitleSensitive stripping voltammetry of heavy metals by using a composite sensor based on a built-in bismuth precursor Analyst 130 971 Occurrence Handle10.1039/b502486m Occurrence Handle1:CAS:528:DC%2BD2MXktlKru7g%3D

    Article  CAS  Google Scholar 

  • Merkoci A, Castaneda M T, Alegret S (2005) Stripping analysis of heavy metals by using mercury free composite based sensors. Pure and applied chemistry edited by research sign post, E. Palomar, p 1

  • Merkoci A, Alegret S (2007) Graphite epoxy composite sensors for stripping analysis. Electrochemical sensor analysis, Elsevier (in press)

  • Merkoci A, Anik Kirgoz U, Alegret S (2007) Determination of lead and cadmium in tap water and soils by stripping analysis using mercury free graphite epoxy composite electrodes. Electrochemical sensor analysis, Elsevier (in press)

  • U Anik Kirgoz H Tural S Timur N Pazarlioglu A Telefoncu R Pilloton (2005) ArticleTitleLaccase biosensors based on mercury thin film electrode Art Cells Blood Subst Biotechnol 33 447 Occurrence Handle10.1080/10731190500290261 Occurrence Handle1:CAS:528:DC%2BD2MXhtFCru7zJ

    Article  CAS  Google Scholar 

  • A Mulchandani S Pan (1999) ArticleTitleFerrocene conjugated m-phenylenediamine conducting polymer-incorporated peroxidase biosensors Anal Biochem 267 141 Occurrence Handle10.1006/abio.1998.2983 Occurrence Handle1:CAS:528:DyaK1MXotlKjtA%3D%3D

    Article  CAS  Google Scholar 

  • S J Updike G P Hicks (1967) ArticleTitleThe enzyme electrode Nature 214 986 Occurrence Handle10.1038/214986a0 Occurrence Handle1:CAS:528:DyaF2sXksVSqtbs%3D

    Article  CAS  Google Scholar 

  • R M Ianniello T J Lindsay A M Yacynych (1982) ArticleTitleDifferential pulse voltammetric study of direct electron transfer in glucose oxidase chemically modified graphite electrodes Anal Chem 54 1098 Occurrence Handle10.1021/ac00244a019 Occurrence Handle1:CAS:528:DyaL38XitVOgsrg%3D

    Article  CAS  Google Scholar 

  • J J Kulys (1981) ArticleTitleDevelopment of new analytical systems based on biocatalyzers Enzyme Microb Technol 3 344 Occurrence Handle10.1016/0141-0229(81)90012-0 Occurrence Handle1:CAS:528:DyaL3MXmtVSju7k%3D

    Article  CAS  Google Scholar 

  • P C Pandey S Upadhyay B Upadhyay (1997) ArticleTitlePeroxide biosensors and mediated electrochemical regeneration of redox enzymes Anal Biochem 252 136 Occurrence Handle10.1006/abio.1997.2291 Occurrence Handle1:CAS:528:DyaK2sXmsVWkt7w%3D

    Article  CAS  Google Scholar 

  • A E G Cass D G Francis H A O Hill W J Aston I J Higgins E V Plotkin L D L Scott A P F Turner (1984) ArticleTitleFerrocene-mediated enzyme electrode for amperometric determination of glucose Anal Chem 56 667 Occurrence Handle10.1021/ac00268a018 Occurrence Handle1:CAS:528:DyaL2cXhtFOhurY%3D

    Article  CAS  Google Scholar 

  • W Sun J Y You Q X Wang K Jiao (2006) ArticleTitleVoltammetric determination of DNA based on its interaction with neutral red Indian J Chem Sect A 45 1173

    Google Scholar 

  • F Davis S P J Higson (2007) ArticleTitleBiofuel cells – recent advances and applications Biosens Bioelectron 22 1224 Occurrence Handle10.1016/j.bios.2006.04.029 Occurrence Handle1:CAS:528:DC%2BD2sXpvVOnsQ%3D%3D

    Article  CAS  Google Scholar 

  • S Timur Y Yigzaw L Gorton (2006) ArticleTitleElectrical wiring of pyranose oxidase with osmium redox polymers Sens Actuators B 113 684 Occurrence Handle10.1016/j.snb.2005.07.017 Occurrence Handle1:CAS:528:DC%2BD28XhtFSjsrs%3D

    Article  CAS  Google Scholar 

  • W Sun M Yang K Jia (2007) ArticleTitleElectrochemical behaviors of neutral red on single and double stranded DNA modified electrode Int J Electrochem Sci 2 93 Occurrence Handle1:CAS:528:DC%2BD2sXkvFKltw%3D%3D

    CAS  Google Scholar 

  • M M Barsan J Klinčar M Batič C M A Brett (2007) ArticleTitleDesign and application of a flow cell for carbon-film based electrochemical enzyme biosensors Talanta 71 1893 Occurrence Handle10.1016/j.talanta.2006.08.032 Occurrence Handle1:CAS:528:DC%2BD2sXjtVWqs70%3D

    Article  CAS  Google Scholar 

  • M E Ghica C M A Brett (2006) ArticleTitleDevelopment of novel glucose and pyruvate biosensors at poly(neutral red) modified carbon film electrodes. Application to natural samples Electroanalysis 18 748 Occurrence Handle10.1002/elan.200503468 Occurrence Handle1:CAS:528:DC%2BD28Xkt1Orsbs%3D

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Chemistry Department, Faculty of Arts and Science, Mugla University, Kötekli, Mugla, Turkey

    Ülkü Anık & Meliha Çubukçu

  2. Biochemistry Department, Faculty of Science, Ege University, Bornova, Izmir/Turkey

    Suna Timur

  3. Nanobioelectronics & Biosensors Group, Departament de Química, Institut Català de Nanotecnologia & Grup de Sensors i Biosensors, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain

    Arben Merkoçi

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  1. Ülkü Anık
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  2. Suna Timur
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  3. Meliha Çubukçu
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  4. Arben Merkoçi
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Correspondence to Ülkü Anık.

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Correspondence: Ülkü Anık, Chemistry Department, Faculty of Arts and Science, Mugla University, TR-48000-Kötekli, Mugla, Turkey

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Anık, Ü., Timur, S., Çubukçu, M. et al. The usage of a bismuth film electrode as transducer in glucose biosensing. Microchim Acta 160, 269–273 (2008). https://doi.org/10.1007/s00604-007-0868-y

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  • DOI: https://doi.org/10.1007/s00604-007-0868-y

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