Skip to main content

Advertisement

SpringerLink
Log in

Pulmonary endothelial chimerism after hematopoietic stem cell transplantation

  • Original Article
  • Published:
Surgery Today Aims and scope Submit manuscript

Abstract

Purpose

Few studies have investigated pulmonary endothelial chimerism after hematopoietic stem cell transplantation. In the present study, we investigated pulmonary endothelial chimerism using the ABH histo-blood group antigen as an identifying marker in cases of ABO-incompatible hematopoietic stem cell transplantation.

Methods

Sixteen lung samples were analyzed. Of these, seven were explanted lungs from lung transplant recipients with severe pulmonary chronic graft-versus-host disease (GVHD). The remaining nine were autopsy samples from patients who died from various causes, and six of these nine cases had a diagnosis of pulmonary chronic GVHD. The ABH histo-blood group antigen was used to differentiate donor cells from recipient cells immunohistochemically. We estimated the percentage of vessels positive for donor blood group antigens in comparison with the total number of vessels.

Results

Donor blood group antigens were expressed in the endothelium of 13 samples, all of which were pathologically diagnosed with pulmonary chronic GVHD. The proportion of vessels with donor group antigens ranged from 0.1 to 17.5%. In contrast, no chimeric vessels were observed in the three samples without pulmonary chronic GVHD.

Conclusions

Our results demonstrate that circulating stem cells engraft into the endothelium to a considerable extent in pulmonary chronic GVHD.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Körbling M, Katz RL, Khanna A, et al. Hepatocytes and epithelial cells of donor origin in recipients of peripheral-blood stem cells. N Engl J Med. 2002;346:738–46.

    Article  PubMed  Google Scholar 

  2. Murata H, Janin A, Leboeuf C, et al. Donor-derived cells and human graft-versus-host disease of the skin. Blood. 2007;109:2663–5.

    Article  CAS  PubMed  Google Scholar 

  3. Rookmaaker MB, Tolboom H, Goldschmeding R, Zwaginga J-J, Rabelink TJ, Verhaar MC. Bone marrow-derived cells contribute to endothelial repair after thrombotic microangiopathy. Blood. 2002;99:1095–6.

    Article  CAS  PubMed  Google Scholar 

  4. Deb A, Wang S, Skelding KA, Miller D, Simper D, Caplice NM. Bone marrow-derived cardiomyocytes are present in adult human heart: a study of gender-mismatched bone marrow transplantation patients. Circulation. 2003;107:1247–9.

    Article  PubMed  Google Scholar 

  5. Kleeberger W, Versmold A, Rothämel T, et al. Increased chimerism of bronchial and alveolar epithelium in human lung allografts undergoing chronic injury. Am J Pathol. 2003;162:1487–94.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Albera C, Polak J, Janes S, et al. Repopulation of human pulmonary epithelium by bone marrow cells: a potential means to promote repair. Tissue Eng. 2005;11:1115–21.

    Article  CAS  PubMed  Google Scholar 

  7. Suratt BT, Cool CD, Serls AE, et al. Human pulmonary chimerism after hematopoietic stem cell transplantation. Am J Respir Crit Care Med. 2003;168:318–22.

    Article  PubMed  Google Scholar 

  8. Mattsson J, Jansson M, Wernerson A, Hassan M. Lung epithelial cells and type II pneumocytes of donor origin after allogeneic hematopoietic stem cell transplantation. Transplantation. 2004;78:154–7.

    Article  PubMed  Google Scholar 

  9. Zander DS, Cogle CR, Theise ND, Crawford JM. Donor-derived type II pneumocytes are rare in the lungs of allogeneic hematopoietic cell transplant recipients. Ann Clin Lab Sci. 2006;36:47–52.

    PubMed  Google Scholar 

  10. Krause DS, Theise ND, Collector MI, et al. Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell. 2001;105:369–77.

    Article  CAS  PubMed  Google Scholar 

  11. Bröcker V, Länger F, Fellous TG, et al. Fibroblasts of recipient origin contribute to bronchiolitis obliterans in human lung transplants. Am J Respir Crit Care Med. 2006;173:1276–82.

    Article  PubMed  Google Scholar 

  12. Murata H, Ratajczak P, Meignin V, et al. Endothelial cell chimerism associated with graft rejection after human lung transplantation. Transplantation. 2008;85:150–4.

    Article  PubMed  Google Scholar 

  13. Lagaaij EL, Cramer-Knijnenburg GF, van Kemenade FJ, van Es LA, Bruijn JA, van Krieken JHJM. Endothelial cell chimerism after renal transplantation and vascular rejection. Lancet. 2001;357:33–7.

    Article  CAS  PubMed  Google Scholar 

  14. Tanabe T, Ishida H, Horita S, et al. Endothelial chimerism after ABO-incompatible kidney transplantation. Transplantation. 2012;93:709–16.

    Article  CAS  PubMed  Google Scholar 

  15. Tanaka Y, Haga H, Egawa H, et al. Intragraft expression of recipient type ABO blood group antigens: long term follow up and histological features after liver transplantation. Liver Transpl. 2005;11:547–54.

    Article  PubMed  Google Scholar 

  16. Filipovich AH, Weisdorf D, Pavletic S, et al. National Institutes of Health consensus development project on criteria for clinical trials in chronic graft-versus-host disease: I. Diagnosis and staging working group report. Biol Blood Marrow Transpl. 2005;11:945–56.

    Article  Google Scholar 

  17. Chen F, Yamane M, Inoue M, et al. Less maintenance immunosuppression in lung transplantation following hematopoietic stem cell transplantation from the same living donor. Am J Transpl. 2011;11:1509–16.

    Article  CAS  Google Scholar 

  18. Zander DS. Lung transplantation pathology. In: Ruiz P, editor. Transplantation pathology. New York: Cambridge University Press; 2009. p. 217–48.

    Google Scholar 

  19. Biedermann BC, Sahner S, Gregor M, et al. Endothelial injury mediated by cytotoxic T lymphocytes and loss of microvessels in chronic graft versus host disease. Lancet. 2002;359:2078–83.

    Article  PubMed  Google Scholar 

  20. Ferrara JL, Levine JE, Reddy P, Holler E. Graft-versus-host disease. Lancet. 2009;373:1550–61.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Flowers MED, Inamoto Y, Carpenter PA, et al. Comparative analysis of risk factors for acute graft-versus-host disease and for chronic graft-versus-host disease according to National Institutes of Health consensus criteria. Blood. 2011;117:3214–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Carlens S, Ringden O, Remberger M, et al. Risk factors for chronic graft-versus-host disease after bone marrow transplantation: a retrospective single centre analysis. Bone Marrow Transpl. 1998;22:755–61.

    Article  CAS  Google Scholar 

  23. Ghayur T, Seemayer T, Xenocostas A, Lapp W. Complete sequential regeneration of graft-vs.-host-induced severely dysplastic thymuses. Implications for the pathogenesis of chronic graft-vs.-host disease. Am J Pathol. 1988;133:39–46.

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Weinberg K, Blazar BR, Wagner JE, et al. Factors affecting thymic function after allogeneic hematopoietic stem cell transplantation. Blood. 2001;97:1458–66.

    Article  CAS  PubMed  Google Scholar 

  25. Fukushi N, Arase H, Wang B, et al. Thymus: a direct target tissue in graft-versus-host reaction after allogeneic bone marrow transplantation that results in abrogation of induction of self-tolerance. Proc Natl Acad Sci USA. 1990;87:6301–5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. van den Brink MR, Moore E, Ferrara JL, Burakoff SJ. Graft-versus-host-disease-associated thymic damage results in the appearance of T cell clones with anti-host reactivity1. Transplantation. 2000;69:446–50.

    Article  PubMed  Google Scholar 

  27. Holländer G, Widmer B, Burakoff S. Loss of normal thymic repertoire selection and persistence of autoreactive T cells in graft vs host disease. J Immunol. 1994;152:1609–17.

    PubMed  Google Scholar 

  28. Clave E, Rocha V, Talvensaari K, et al. Prognostic value of pretransplantation host thymic function in HLA-identical sibling hematopoietic stem cell transplantation. Blood. 2005;105:2608–13.

    Article  CAS  PubMed  Google Scholar 

  29. Favre G, Beksaç M, Bacigalupo A, et al. Differences between graft product and donor side effects following bone marrow or stem cell donation. Bone Marrow Transpl. 2003;32:873–80.

    Article  CAS  Google Scholar 

  30. Eapen M, Rocha V, Sanz G, et al. Effect of graft source on unrelated donor haemopoietic stem-cell transplantation in adults with acute leukaemia: a retrospective analysis. Lancet Oncol. 2010;11:653–60.

    Article  PubMed  PubMed Central  Google Scholar 

  31. Katayama H, Kurokawa Y, Nakamura K, et al. Extended Clavien-Dindo classification of surgical complications: Japan Clinical Oncology Group postoperative complications criteria. Surg Today. 2016;46:668–85.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Thoracic Surgery, Kyoto University, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan

    Kyoko Hijiya, Toyofumi Fengshi Chen-Yoshikawa, Akihiro Aoyama & Hiroshi Date

  2. Department of Diagnostic Pathology, Kyoto University Hospital, Kyoto, Japan

    Aya Miyagawa-Hayashino & Hironori Haga

  3. Department of Hematology and Oncology, Kyoto University Hospital, Kyoto, Japan

    Tadakazu Kondo & Akifumi Takaori-Kondo

Authors
  1. Kyoko Hijiya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Toyofumi Fengshi Chen-Yoshikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aya Miyagawa-Hayashino
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tadakazu Kondo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Akihiro Aoyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hironori Haga
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Akifumi Takaori-Kondo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hiroshi Date
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kyoko Hijiya.

Ethics declarations

Conflict of interest

The authors have no conflicts of interest to disclose.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hijiya, K., Chen-Yoshikawa, T.F., Miyagawa-Hayashino, A. et al. Pulmonary endothelial chimerism after hematopoietic stem cell transplantation. Surg Today 48, 101–109 (2018). https://doi.org/10.1007/s00595-017-1562-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00595-017-1562-2

Keywords

Search

Navigation