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Experimental orthotopic lung transplantation model in rats with cold storage

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Abstract

This report describes a new experimental procedure, a rat unilateral, orthotopic lung transplantation with cold storage, and evaluates its relevancy and reliability to study the early events during cold ischemia/reperfusion (I/R) injury. This model, using the cuff technique, does not require extensive training and is relatively easy to be established. The model can induce reproducible degrees of pulmonary graft injury including impaired gas exchange, proinflammatory cytokine upregulation, or inflammatory infiltrates, depending on the preservation time. The results are consistent with the previous clinical evidence, thus suggesting that this model is a valid and reliable animal model of cold I/R injury.

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Authors and Affiliations

  1. Heart, Lung and Esophageal Surgery Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA

    Ryujiro Sugimoto, Atsunori Nakao, Junichi Kohmoto, Jianghua Zhan & Kenneth R. McCurry

  2. Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA

    Ryujiro Sugimoto, Atsunori Nakao, Junichi Kohmoto, Jianghua Zhan & Kenneth R. McCurry

  3. Department of Thoracic Surgery, Okayama University Hospital, Okayama, Japan

    Ryujiro Sugimoto, Itaru Nagahiro, Junichi Kohmoto, Seiichiro Sugimoto, Mikio Okazaki, Masaomi Yamane, Hidetoshi Inokawa, Takahiro Oto & Yoshifumi Sano

  4. Department of Pediatric Surgery, Dokkyo Medical University Koshigaya Hospital, Koshigaya, Japan

    Kazunori Tahara

  5. Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, E1551 Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA, 15213, USA

    Ryujiro Sugimoto, Atsunori Nakao & Kenneth R. McCurry

Authors
  1. Ryujiro Sugimoto
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  2. Atsunori Nakao
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  3. Itaru Nagahiro
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  4. Junichi Kohmoto
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  5. Seiichiro Sugimoto
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  6. Mikio Okazaki
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  7. Masaomi Yamane
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  8. Hidetoshi Inokawa
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  9. Takahiro Oto
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  10. Kazunori Tahara
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  11. Jianghua Zhan
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  12. Yoshifumi Sano
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  13. Kenneth R. McCurry
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Additional information

A. Nakao and K.R. McCurry contributed equally to this work.

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Sugimoto, R., Nakao, A., Nagahiro, I. et al. Experimental orthotopic lung transplantation model in rats with cold storage. Surg Today 39, 641–645 (2009). https://doi.org/10.1007/s00595-008-3929-x

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  • DOI: https://doi.org/10.1007/s00595-008-3929-x

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