Abstract
This study evaluated the effects of N-acetylcysteine as a scavenger of radical oxygen species on myocardial injury as a remote organ after skeletal muscle ischaemia–reperfusion. Twenty male Wistar rats were allocated randomly into two experimental groups: ischaemia–reperfusion and ischaemia–reperfusion + N-acetylcysteine. All animals underwent 2 h of ischaemia by occlusion of the femoral artery followed by 24 h of reperfusion. Rats treated with N-acetylcysteine were given an intravenous dose of 150 mg/kg, immediately before reperfusion. After the reperfusion period, animals were euthanized and hearts harvested for histopathological analysis under light microscopy. In the ischaemia–reperfusion group, tissues showed histological changes with interstitial oedema, neutrophil infiltration and adhesion of neutrophils to the endothelium, haemorrhage and coagulative necrosis. Histopathologically, there was a significant difference (P < 0.05) between the two groups. The administration of N-acetylcysteine significantly decreased myocardial injury induced by skeletal muscle ischaemia–reperfusion according to our histological findings.
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References
Abe M, Takiguchi Y, Ichimaru S, Tsuchiya K, Wada K (2008) Comparison of the protective effect of N-acetylcysteine by different treatments on rat myocardial ischemia–reperfusion injury. J Pharmacol Sci 106:571–577
Alberola A, Such L, Gil F, Zaragoza R, Morcillo EJ (1991) Protective effect of N-acetylcysteine on ischaemia-induced myocardial damage in canine heart. Naunyn Schmiedeberg’s Arch Pharmacol 343:505–510
Aruoma OI, Halliwell B, Hoey BM, Butler J (1989) The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radic Biol Med 6:593–597
Barry MC, Kelly CJ, Abdih H, Watson RW, Stapleton P, Sheehan SJ, Redmond HP, Hayes DB (1997) Differential effects of lower limb revascularisation on organ injury and the role of the amino acid taurine. Eur J Vasc Endovasc Surg 13:193–201
Cabeza J, Motilva V, Martin MJ, de la Lastra CA (2001) Mechanisms involved in gastric protection of melatonin against oxidant stress by ischemia–reperfusion in rats. Life Sci 68:1405–1415
Crinnion JN, Homer-Vanniasinkam S, Parkin SM, Gough MJ (1996) Role of neutrophil-endothelial adhesion in skeletal muscle reperfusion injury. Br J Surg 83:251–254
Cuzzocrea S, Mazzon E, Costantino G, Serraino I, De Sarro A, Caputi AP (2000) Effects of N-acetylcysteine in a rat model of ischemia and reperfusion injury. Cardiovasc Res 47(3):537–548
De Vries N, De Flora S (1993) N-acetyl-l-cysteine. J Cell Biochem 17:270–277
Demir S, Inal-Erden M (1998) Pentoxifylline and N-acetylcysteine in hepatic ischemia/reperfusion injury. Clin Chim Acta 275:127–135
Dhalla NS, Elmoselhi AB, Hata T, Makino N (2000) Status of myocardial antioxidants in ischemia–reperfusion injury. Cardiovasc Res 47:446–456
Ferrante RJ, Hobson RW, Miyasaka M, Granger DN, Duran WN (1996) Inhibition of white blood cell adhesion at reperfusion decreases tissue damage in postischemic striated muscle. J Vasc Surg 24:187–193
Granger DN (1988) Role of xanthine oxidase and granulocytes in ischemia–reperfusion injury. Am J Physiol 255:H1269–H1275
Granger DN, Korthuis RJ (1995) Physiologic mechanisms of postischemic tissue injury. Annu Rev Physiol 57:311–332
Holodines MR (1991) Clinical pharmacokinetics of N-acetylcysteine. Clin Pharmacokinet 20(2):123–134
Irmak MK, Koltuksuz U, Kutlu NO, Yagmurca M, Ozyurt H, Karaman A, Okyol O (2001) The effect of caffeic acid phenethyl ester on ischemia–reperfusion injury in comparison with alpha-tocopherol in rat kidneys. Urol Res 29:190–193
Irmak MK, Fadillioglu E, Sogut S, Erdogan H, Gulec M, Ozer M, Yagmurca M, Gozukara ME (2003) Effects of caffeic acid phenethyl ester and alpha-tocopherol on reperfusion injury in rat brain. Cell Biochem Funct 21:283–289
Kearns SR, Kelly CJ, Barry M, Abdih H, Condron C, Leahy A, Bouchier-Hayes D (1999) Vitamin C reduces ischaemia reperfusion-induced acute lung injury. Eur J Vasc Endovasc Surg 17:533–536
McCord JM (1985) Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 312:159–163
Menasché P, Grousset C, Gauduel Y, Mouas C, Piwnica A (1992) Maintenance of the myocardial thiol pool by N-acetylcysteine. An effective means of improving cardioplegic protection. J Thorac Cardiovasc Surg 103(5):936–944
Milazzo VJ, Ferrante RJ, Sabido F, Silva MB Jr, Hobson RW II, Duran WN (1996) Time course of leukocyte adhesion to endothelium in ischemia–reperfusion. J Surg Res 61:139–142
Ng TB, Liu F, Zhao L (2000) Antioxidative and free radical scavenging activities of pineal indoles. J Neural Transm 107:1243–1251
Okatani Y, Wakatsuki A, Reiter RJ, Enzan H, Miyahara Y (2003) Protective effect of melatonin against mitochondrial injury induced by ischemia and reperfusion in rat liver. Eur J Pharmacol 469:145–152
Papoutsidakis N, Arkadopoulos N, Smyrniotis V, Tzanatos H, Kalimeris K, Nastos K, Defterevos G, Pafiti A, Kostopanagiotou G (2011) Early myocardial injury is an integral component of experimental acute liver failure—a study in two porcine models. Arch Med Sci 2:217–223
Parks DA, Granger DN (1986) Contributions of ischemia and reperfusion to mucosal lesion formation. Am J Physiol 250:749–753
Romson JL, Hook BG, Kunkel SL, Abrams GD, Schork MA, Lucchesi BR (1983) Reduction of the extent of ischemic myocardial injury by neutrophil depletion in the dog. Circulation 67(5):1016–1023
Sadasivan KK, Carden DL, Moore MB, Korthuis RJ (1997) Neutrophil mediated microvascular injury in acute, experimental compartment syndrome. Clin Orthop 339:206–215
Sochman J, Kolc J, Vrána M, Fabián J (1990) Cardioprotective effects of N-acetylcysteine: the reduction in the extent of infarction and occurrence of reperfusion arrhythmias in the dog. Int J Cardiol 28:191–196
Sotoudeh A, Takhtfooladi MA, Jahanshahi A, KhiabanianAsl AH, Takhtfooladi HA, Khansari M (2012) Effect of N-acetylcysteine on lung injury induced by skeletal muscle ischemia–reperfusion: histopathological study in rat model. Acta Cir Bras 27(2):168–171
Takhtfooladi MA, Jahanshahi A, Jahanshahi GH, Sotoudeh A, Takhtfooladi HA, Khansari M (2012) Protective effect of N-acetylcysteine on kidney as a remote organ after skeletal muscle ischemia–reperfusion. Acta Cir Bras 27(9):611–615
Uz E, Sogut S, Sahin S, Var A, Ozyurt H, Güleç M, Akyol O (2002) The protective role of caffeic acid phenethyl ester (CAPE) on testicular tissue after testicular torsion and detorsion. World J Urol 20:264–270
Welbourn CR, Goldman G, Paterson IS, Valeri CR, Shepro D, Hechtman HB (1991) Pathophysiology of ischaemia reperfusion injury: central role of the neutrophil. Br J Surg 78:651–655
Xia Y, Khatchikian G, Zweier JL (1996) Adenosine deaminase inhibition prevents free radical-mediated injury in the post-ischemic heart. Am Soc Biochem Mol Biol 271:10096–10102
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Takhtfooladi, M.A., Jahanshahi, G., Sotoudeh, A. et al. Protective effects of N-acetylcysteine on myocardial injury induced by hindlimb ischaemia–reperfusion: a histological study in a rat model. Comp Clin Pathol 23, 1237–1240 (2014). https://doi.org/10.1007/s00580-013-1768-7
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DOI: https://doi.org/10.1007/s00580-013-1768-7