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Study of T-cell immunoglobulin- and mucin-domain-containing molecule 3 polymorphisms in Egyptian childhood immune thrombocytopenic purpura

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Abstract

The T-cell immunoglobulin- and mucin-domain-containing molecules (TIMs) influence immune regulation. TIM-3, a transmembrane protein expressed on terminally differentiated Th1 cells, is a central regulator of T-cell responses. Dysfunctional T-lymphocyte immunity plays an important role in the pathophysiology of immune thrombocytopenic purpura (ITP). The study aimed to evaluate the possible contribution of TIM-3 polymorphisms to the pathogenesis of ITP and their relation to age of disease onset, clinical course, and response to therapy. Genotyping of TIM-3 −1516G>T, −574T>G, and 4259G>T was performed in 100 childhood ITP patients and 210 healthy individuals by polymerase chain reaction-restriction fragment length polymorphism. No significant differences existed in genotype and allele distributions between patients and controls in all three sites. There was a statistically significant association between the −574T>G T/T genotype and both a lower platelet count and a younger age of disease onset (p = 0.001). A near significant association was found between the 4259G>T T/T genotype and increased disease severity at diagnosis and also between both −574T>G G/G and 4259G>T T/T and a better quality of response (p = 0.07). The study did not detect a risk association between the TIM-3 single-nucleotide polymorphisms and ITP susceptibility. However, it is associated with an earlier disease onset and severity as indicated by the lower platelet count.

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Acknowledgments

This work was supported by a fund from Cairo University, Egypt.

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Authors and Affiliations

  1. Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, 18 Muthaf El-Manial St., Cairo, Egypt, 11451

    Eman R. Radwan, Eman A. Youssef, Nadia I. Sewelam & Hanaa H. Arnaout

  2. Department of Paediatric Haematology, Faculty of Medicine, Cairo University, Cairo, Egypt

    Rania I. M. Goda

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  1. Eman R. Radwan
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  2. Eman A. Youssef
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  3. Nadia I. Sewelam
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  4. Rania I. M. Goda
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  5. Hanaa H. Arnaout
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Correspondence to Eman R. Radwan.

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Radwan, E.R., Youssef, E.A., Sewelam, N.I. et al. Study of T-cell immunoglobulin- and mucin-domain-containing molecule 3 polymorphisms in Egyptian childhood immune thrombocytopenic purpura. Comp Clin Pathol 23, 933–939 (2014). https://doi.org/10.1007/s00580-013-1716-6

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  • DOI: https://doi.org/10.1007/s00580-013-1716-6

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