Abstract
Transportation is an inevitable husbandry practice which animals encounter in the livestock industry and raises considerable interest both in economic and animal welfare terms. With respect to the growing interest in using acute phase proteins as health and welfare indicators, the aim of the present study was to investigate the impact of road transportation on the concentrations of fibrinogen, haptoglobin, serum amyloid A, and ceruloplasmin in Iranian dromedary camels. Ten clinically healthy Iranian dromedary camels, five males and five females, were selected and subjected to a journey of approximately 300 km in a truck by road during the late summer. Blood samples were collected immediately before loading at 8:30 a.m., after 1 h transportation, at 9:30 a.m., and at the end of the journey after unloading at 1:30 pm. A final blood sample was taken 24 h after arrival. Blood concentrations of plasma proteins, including fibrinogen, haptoglobin, serum amyloid A, ceruloplasmin, total protein, and albumin, were measured using validated methods. Mean basal pre-transport concentrations of fibrinogen (3.10 ± 0.47 g/l), haptoglobin (0.3 ± 0.04 g/l), serum amyloid A (9.77 ± 0.62 μg/ml), and ceruloplasmin (0.096 ± 0.01 g/l) did not change significantly during and after road transportation. In addition, transportation had no significant effects on basal plasma concentrations of total protein (60.8 ± 6.1 g/l) and albumin (38.3 ± 2.1 g/l). No significant difference was observed in any parameter between sexes at each sampling time. We conclude that stressful conditions during the journey under hot summer environmental conditions were not severe enough to trigger an acute phase response. The value of the investigated plasma parameters as welfare indicators in dromedary camel over short transportation would be limited. Taken together, this plasma protein profile may be of value for future research on acute phase proteins as potential markers of health and welfare and to develop a baseline for practical applications in dromedary camels.
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References
Alsemgeest SPM, Lambooy IE, Wierenga HK, Dieleman SJ, Meerkerk B, van Ederen AM, Niewold TA (1995) Influence of physical stress on the plasma concentrations of serum amyloid A (SAA) and haptoglobin (Hp) in calves. Vet Q 17:9–12
Alsemgeest SPM, van't Klooster GAE, van Miert ASJPAM, Hulskamp-Koch CK, Gruys E (1996) Primary bovine hepatocytes in the study of cytokine induced acute-phase protein secretion in vitro. Vet Immunol Immunopathol 53:179–184. doi:10.1016/0165-2427(96)05602-4
Arthington JD, Eicher SD, Kunkle WE, Martin FG (2003) Effect of transportation and commingling on the acute-phase protein response, growth, and feed intake of newly weaned beef calves. J Anim Sci 81:1120–1125
Atkinson PJ (1992) Investigation of the effects of transport and lairage on hydration state and resting behaviour of calves for export. Vet Rec 130:413–416
Averós X, Martín S, Riu M, Serratosa J, Gosálvez LF (2008) Stress response of extensively reared young bulls being transported to growing-finishing farms under Spanish summer commercial conditions. Livest Sci 119:174–182. doi:10.1016/j.livsci.2008.04.002
Averós X, Herranz A, Sánchez R, Gosálvez LF (2009) Effect of the duration of commercial journeys between rearing farms and growing–finishing farms on the physiological stress response of weaned piglets. Livest Sci. doi:10.1016/j.livsci.2008.09.019
Broom DM (2000) Welfare assessment and welfare problem areas during handling and transport. In: Grandin T (ed) Livestock handling and transport, 2nd edn. CAB International, Wallingford, pp 43–61
Broom DM, Johnson KG (1993) Stress and animal welfare. Chapman and Hall, London
Cook CJ, Mellor DJ, Harris PJ, Ingram JR, Matthews LR (2000) Hands-on and hands-off measurement of stress. In: Moberg GP, Mench JA (eds) The biology of animal stress: basic principles and implications for animal welfare. CAB International, Wallingford, p 123
Deak T, Meriwether JL, Fleshner M, Spencer RL, Abouhamze A, Moldawer LL, Grahn RE, Watkins LR, Maier SF (1997) Evidence that brief stress may induce the acute phase response in rats. Am J Physiol 273:1998–2004
Duncan JR, Prasse KW, Mahaffey EA (1994) Veterinary laboratory medicine clinical pathology. Iowa State Univ. Press, Ames
Earley B, Fisher AD, O'Riordan EG (2006) Effects of pre-transport fasting on the physiological responses of young cattle to 8-hour road transport. Ir J Agric Food Res 45:51–60
Eckersall PD (2000) Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Rev Med Vet 151:577–584
Essamadi AK, Bengoumi M, Zaoui D, Faye B, Bellenchi GC, Musci G, Calabrese L (2002) Purification and partial characterization of camel (Camelus dromedarius) ceruloplasmin. Comp Biochem Physiol B 131:509–517. doi:10.1016/S1096-4959(02)00030-1
Feldman BF, Zinkl JG, Jain NC (2000) Schalm's veterinary hematology. Lippincott Williams & Wilkins, Philadelphia, pp 891–896
Fleming RE, Gitlin JD (1990) Primary structure of rat ceruloplasmin and analysis of tissue-specific gene expression during development. J Biol Chem 265:7701–7707
Gentry PA (2004) Comparative aspects of blood coagulation. Vet J 168:238–251. doi:10.1016/j.tvjl.2003.09.013
Hickey MC, Drennan M, Early B (2003) The effect of abrupt weaning of suckler calves on the plasma concentrations of cortisol, catecholamines, leukocytes, acute-phase proteins and in vitro interferon-gamma production. J Anim Sci 81:2847–2855
Hicks TA, Mcglone JJ, Whisnant CS, Kattesh HG, Norman RL (1998) Behavioral, endocrine, immune and performance measures for pigs exposed to acute stress. J Anim Sci 76:474–483
Higgins AJ, Kock RA (1984) A guide to the clinical examination, chemical restraint and medication of the camel. Br Vet J 140:485–504
Higuchi H, Katoh N, Miyamoto T, Uchida E, Yuasa A, Takahashi K (1994) Dexamethasone-induced haptoglobin release by calf liver parenchymal cells. Am J Vet Res 55:1080–1085
Hirvonen J, Hietakorpi S, Saloniemi H (1997) Acute phase response in emergency slaughtered dairy cows. Meat Sci 46:29–257. doi:10.1016/S0309-1740(97)00020-X
Hurtung J (2003) Effects of transport on health of farm animals. Vet Res Commun 27:525–527. doi:10.1023/B:VERC.0000014212.81294.78
Hussein MF, Al-Momen AK, Gader AMA (1992) Haemostatic parameters in the camel (Camelus dromedarius): comparison with humans. Comp Haematol Int 2:92–96. doi:10.1007/BF00186267
Kadim IT, Mahgoub O, Al-Kindi A, Al-Marzooqi W, Al-Saqri NM (2006) Effects of transportation at high ambient temperatures on physiological responses, carcass and meat quality characteristics of three breeds of Omani goats. Meat Sci 73:626–634. doi:10.1016/j.meatsci.2006.03.003
Kaneko JJ (1997) Serum proteins and dysproteinemias. In: Kaneko JJ, Harvey JW, Bruss M (eds) Clinical biochemistry of domestic animals, 5th edn. Academic, San Diego, pp 117–138
Kincaid RL, Gay CC, Krieger RI (1986) Relationship of serum and plasma copper and ceruloplasmin concentrations of cattle and the effects of whole blood sample storage. Am J Vet Res 47:1157–1159
Knowles TG, Warriss PD, Brown SN, Edwards JE (1999) Effects on cattle of transportation by road for up to 31 hours. Vet Rec 145:575–582
Knowles TG, Warriss PD, Brown SN, Edwards JE, Perry AM, Watkins PE, Philips AJ (1996) The effects of feeding, watering and resting intervals on lambs exported by road and ferry to France. Vet Rec 139:335–339
Lomborg SR, Nielsen LR, Heegaard PMH, Jacobsen S (2008) Acute phase proteins in cattle after exposure to complex stress. Vet Res Commun 32:575–582. doi:10.1007/s11259-008-9057-7
Mehrotra V, Gupta ML (1989) Seasonal variation in certain blood constituents in camel. Indian J Anim Sci 59:1559–1560
Minka NS, Ayo JO (2007) Effects of loading behaviour and road transport stress on traumatic injuries in cattle transported by road during the hot-dry season. Livest Sci 107:91–95. doi:10.1016/j.livsci.2006.10.013
Mitchell GB, Clark ME, Siwicky M, Caswell JL (2008) Stress alters the cellular and proteomic compartments of bovine bronchoalveolar lavage fluid. Vet Immunol Immunopathol 125:111–125. doi:10.1016/j.vetimm.2008.05.005
Mohammadi GR, Nazifi S, Rezakhani A, Esmailnejad Z (2007) Effect of transportation stress on blood and bronchoalveolar lavage fluid components in calves. Comp Clin Pathol 16:85–95. doi:10.1007/s00580-007-0673-3
Murata H (2007) Stress and acute phase protein response: an inconspicuous but essential linkage. Vet J 173:473–474. doi:10.1016/j.tvjl.2006.05.008
Murata H, Miyamoto T (1993) Bovine haptoglobin as a possible immunomodulator in the sera of transported calves. Br Vet J 149:277–283
Murata H, Shimada N, Yoshioka M (2004) Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J 168:28–40. doi:10.1016/S1090-0233(03)00119-9
Nazifi S, Saeb M, Ghasemian O, Esmailnezhad Z (2006) Evaluation of serum haptoglobin in clinically healthy Iranian camels (Camelus dromedarius). Comp Clin Pathol 15:195–197. doi:10.1007/s00580-006-0640-4
Parrott RF, Hall SJG, Lloyd DM (1998) Heart rate and stress hormone responses of sheep to road transport following two different loading procedures. Anim Welf 7:257–267
Piñeiro M, Pineiro C, Carpintero R, Morales J, Campbell FM, Eckersall PD, Toussaint MJ, Lampreave F (2007) Characterization of the pig acute phase protein response to road transport. Vet J 173:669–674. doi:10.1016/j.tvjl.2006.02.006
Petersen HH, Nielsen JP, Heegaard PMH (2004) Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res 35:163–187. doi:10.1051/vetres:2004002
Phillips WA (1984) The effect of assembly and transit stressors on plasma fibrinogen concentration of beef calves. Can J Comp Med 48:35–41
Qiu X, Arthington JD, Riley DG, Chase CC Jr, Phillips WA, Coleman SW, Olson TA (2007) Genetic effects on acute phase protein response to the stresses of weaning and transportation in beef calves. J Anim Sci 85:2367–2374. doi:10.2527/jas.2006-843
Schaefer AL, Jones SDM, Tont AKW, Young BA, Murray NL, Lepage P (1992) Effects of post transport electrolyte supplementation on tissue electrolytes, hematology, urine osmolality and weight loss in beef bulls. Livest Prod Sci 30:333–345. doi:10.1016/0301-6226(92)90042-3
Shalah MR (1983) The role of camels in overcoming world meat shortage. Egypt J Vet Sci 20:101–110
Smith BL, Jones JH, Hornof WJ, Miles JA, Longworth KE, Willits NH (1996) Effect of road transport on indices of stress in horses. Equine Vet J 28:446–454
Sporer KRB, Weber PSD, Burton JL, Earley B, Crowe MA (2008) Transportation of young beef bulls alters circulating physiological parameters that may be effective biomarkers of stress. J Anim Sci 86:1325–1334. doi:10.2527/jas.2007-0762
Sunderman FW Jr, Nomoto S (1970) Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clin Chem 160:903–907
Uhlar CM, Whitehead AS (1999) Serum amyloid A, the major vertebrate acute-phase reactant. Eur J Biochem 265:501–523. doi:10.1046/j.1432-1327.1999.00657.x
Warris PD (1998) Choosing appropriate space allowance for slaughter pigs transported by road: a review. Vet Rec 142:449–454
Warris PD (2003) Optimal lairage times and conditions for slaughter pigs: a review. Vet Rec 153:170–176
Wernery U, Kaaden OR (2002) Infectious Diseases in Camelides, 2nd edn. Blackwell Wissenschafts, Berlin, p 97
Yagil R (1982) Camels and camel milk. FAO Animal Production and Health. Publications Division, Food and Agriculture Organization of the United Nations. Via delle Terme di Caracalla, 00100 Rome, Italy (No. 26)
Yousif OK, Babiker SA (1989) The desert camel as meat animals. Meat Sci 26:245–254. doi:10.1016/0309-1740(89)90010-7
Zongping L (2003) Studies on the haematology and trace element status of adult bactrian camels (Camelus bactrianus) in China. Vet Res Commun 27:397–405. doi:10.1023/A:1024762205249
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The authors gratefully acknowledge the staff at the Camel Research Institute for their kind help in this study.
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Baghshani, H., Nazifi, S., Saeb, M. et al. Influence of road transportation on plasma concentrations of acute phase proteins, including fibrinogen, haptoglobin, serum amyloid A, and ceruloplasmin, in dromedary camels (Camelus dromedarius). Comp Clin Pathol 19, 193–198 (2010). https://doi.org/10.1007/s00580-009-0839-2
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DOI: https://doi.org/10.1007/s00580-009-0839-2