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Ectomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densiflora seedlings that resembles naturally occurring ‘shiro’

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Abstract

We established an in vitro ectomycorrhizal symbiosis between Tricholoma matsutake and Pinus densiflora. Mycorrhiza formed in a substrate of Modified Norkrans' C medium and granite-based soil had features similar to those observed previously only in naturally occurring mycorrhizal system called ‘shiro,’ and promoted the growth of plants with smaller root/shoot ratios. The in vitro formation of ‘shiro’ is essential for the development of T. matsutake system to produce mushrooms and is useful for the propagation and plantation of the mycorrhizal seedlings.

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Acknowledgements

This work was supported by grants from Shinshu University, Ibaraki prefecture, and the Ministry of Agriculture, Forestry, and Fisheries of Japan.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Minami-minowa, Nagano, 399-4598, Japan

    Akiyoshi Yamada

  2. Ibaraki Prefectural Forestry Institute, Naka, Ibaraki, 311-0122, Japan

    Ken Maeda & Hisayasu Kobayashi

  3. Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687, Japan

    Hitoshi Murata

Authors
  1. Akiyoshi Yamada
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  2. Ken Maeda
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  3. Hisayasu Kobayashi
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  4. Hitoshi Murata
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Correspondence to Akiyoshi Yamada.

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Yamada, A., Maeda, K., Kobayashi, H. et al. Ectomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densiflora seedlings that resembles naturally occurring ‘shiro’. Mycorrhiza 16, 111–116 (2006). https://doi.org/10.1007/s00572-005-0021-x

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  • DOI: https://doi.org/10.1007/s00572-005-0021-x

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