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Development of microsatellite markers from an ectomycorrhizal fungus, Tricholoma matsutake, by an ISSR-suppression-PCR method

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Abstract.

An inter-simple sequence repeat (ISSR)-suppression-PCR technique established to develop microsatellite markers of plant species was applied to an ectomycorrhizal fungus, Tricholoma matsutake. Six polymorphic SSR markers were developed. All six polymorphic SSR markers were single-locused and co-dominant. Alleles produced by these six single-locused markers ranged from two to nine per locus and the expected heterozygosities were calculated as values from 0.098 to 0.803. The results indicated that the ISSR-suppression-PCR technique was effective and applicable to the development of microsatellite markers from ectomycorrhizal fungi. Furthermore, the six microsatellite loci did not amplify DNA from any other ectomycorrhizal species investigated, except for Tricholoma nauseosum (Swedish matsutake) and Tricholoma fulvocastaneum, suggesting that population genetics and reproduction of T. matsutake could be investigated by the SSR markers developed in the present study.

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Authors and Affiliations

  1. Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1–1–8, Nishitokyo-shi, Tokyo 188–0002, Japan, , , , ,

    Chunlan Lian & Taizo Hogetsu

  2. Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1–1–1, Bunkyo-ku, Tokyo 113–8657, Japan, , , , ,

    Norihisa Matsushita, Alexis Guerin-Laguette & Kazuo Suzuki

  3. Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Minami-minowa mura, Kamiina gun, Nagano 399–4598, Japan, , , , ,

    Akiyoshi Yamada

Authors
  1. Chunlan Lian
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  2. Taizo Hogetsu
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  3. Norihisa Matsushita
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  4. Alexis Guerin-Laguette
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  5. Kazuo Suzuki
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  6. Akiyoshi Yamada
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Lian, C., Hogetsu, T., Matsushita, N. et al. Development of microsatellite markers from an ectomycorrhizal fungus, Tricholoma matsutake, by an ISSR-suppression-PCR method. Mycorrhiza 13, 27–31 (2003). https://doi.org/10.1007/s00572-002-0193-6

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  • DOI: https://doi.org/10.1007/s00572-002-0193-6

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