Abstract
The number of infertile couples considering using assisted reproductive technologies (ARTs) is growing. Several key indices, such as sperm concentration and motility, are considered when determining an appropriate technique among the existing ARTs. While microscopy is the only way to observe sperms, this method tends to overlook the actual swimming ability of sperms because sperms can be observed only within a very narrow field of view (FOV). In this paper, we propose a microfluidic chip capable of measuring the motility of sperms by inducing the actual swimming ability of sperms in microchannels. To determine whether sperms swim by themselves and reach the target point, 5–10 min is required in an incubator at 37 °C, which inevitably causes the evaporation of the fluid at the microfluidic chip inlet or outlet. A unique structure has been added to the microfluidic chip to prevent unwanted fluid flow due to evaporation, and counting and sorting capabilities of the fabricated device have been experimentally demonstrated. The microfluidic chip is shown to have a good agreement with commercial chips in total sperm counting. Another feature of sorting motile and progressive sperm to 95% on one chip is also verified. This feature differentiates our solution from the existing commercial chips and can help increase the success rate of ARTs. The developed MFC can provide a way to determine the actual swimming motility of sperms using a microscope in small clinics or a portable kit which is publicly available without the expensive sperm analysis equipment.
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References
Arora M (2013) Cell culture media: a review. Mater Methods 3(175):24. https://doi.org/10.13070/mm.en.3.175
Bahadur G, Homburg R, Muneer A, Racich P, Alangaden T, Al-Habib A, Okolo S (2016) First line fertility treatment strategies regarding IUI and IVF require clinical evidence. Hum Reprod 31(6):1141–1146. https://doi.org/10.1093/humrep/dew075
Bajaj B (2019) IUI or IVF: in resource crunch countries. J Basic Clin Reprod Sci 8(1):9–13. https://doi.org/10.4103/2278-960X.194517
Berthier E, Beebe DJ (2007) Flow rate analysis of a surface tension driven passive micropump. Lab Chip 7(11):1475–1478. https://doi.org/10.1039/b707637a
Berthier E, Warrick J, Yu H, Beebe DJ (2008) Managing evaporation for more robust microscale assays: part 2. Characterization of convection and diffusion for cell biology. Lab Chip 8(6):860–864. https://doi.org/10.1039/b717423c
Chen YA, Huang ZW, Tsai FS, Chen CY, Lin CM, Wo AM (2011) Analysis of sperm concentration and motility in a microfluidic device. Microfluid Nanofluid 10(1):59–67. https://doi.org/10.1007/s10404-010-0646-8
Chinnasamy T, Kingsley JL, Inci F, Turek PJ, Rosen MP, Behr B, Demirci U (2018) Guidance and self-sorting of active swimmers: 3D periodic arrays increase persistence length of human sperm selecting for the fittest. Adv Sci. https://doi.org/10.1002/advs.201700531
Dearing C, Jayasena C, Lindsay K (2019) Can the sperm class analyser (SCA) CASA-Mot system for human sperm motility analysis reduce imprecision and operator subjectivity and improve semen analysis? Hum Fertil 1:1–11. https://doi.org/10.1080/14647273.2019.1610581
Eamer L, Vollmer M, Nosrati R, San Gabriel MC, Zeidan K, Zini A, Sinton D (2016) Turning the corner in fertility: high DNA integrity of boundary-following sperm. Lab Chip 16(13):2418–2422. https://doi.org/10.1039/C6LC00490C
Hidayatullah P, Mengko TL, Munir R (2017) A survey on multisperm tracking for sperm motility measurement. Int J Mach Learn Comput 7(5):144–151. https://doi.org/10.18178/ijmlc.2017.7.5.637
Huang HY, Huang PW, Yao DJ (2017) Enhanced efficiency of sorting sperm motility utilizing a microfluidic chip. Microsyst Technol 23(2):305–312. https://doi.org/10.1007/s00542-015-2495-6
Inhorn MC, Patrizio P (2014) Infertility around the globe: new thinking on gender, reproductive technologies and global movements in the 21st century. Hum Reprod Update 21(4):411–426. https://doi.org/10.1093/humupd/dmv016
Ju J, Park JY, Kim KC, Kim H, Berthier E, Beebe DJ, Lee SH (2008) Backward flow in a surface tension driven micropump. J Micromechanics Microengineering 18(8):7. https://doi.org/10.1088/0960-1317/18/8/087002
Kandavel V, Cheong Y (2018) Does intra-uterine insemination have a place in modern ART practice? Best Pract Res 53:3–10. https://doi.org/10.1016/j.bpobgyn.2018.08.003
Kim YJ, Chun K (2019) Lensless Imaging Sensor Kit for Sperm Counting with Microfluidic Chip. SAS 2019–2019 IEEE Sensors Applications Symposium, Conference Proceedings, 1–4. https://doi.org/10.1109/SAS.2019.8706105
Knowlton SM, Sadasivam M, Tasoglu S (2015) Microfluidics for sperm research. Trends Biotechnol 33(4):221–229. https://doi.org/10.1016/j.tibtech.2015.01.005
Koyama S, Amarie D, Soini HA, Novotny MV, Jacobson SC (2006) Chemotaxis assays of mouse sperm on microfluidic devices. Anal Chem 78(10):3354–3359
Li Z, Liu W, Qiu T, Xie L, Chen W, Liu R, Cheng J (2014) The construction of an interfacial valve-based microfluidic chip for thermotaxis evaluation of human sperm. Biomicrofluidics 8(2):1–11. https://doi.org/10.1063/1.4866851
Li J, Ning B, Cao X, Luo Y, Guo L, Wei G, Li Y (2016) Separation of motile sperm for in vitro fertilization from frozen-thawed bull semen using progesterone induction on a microchip. Anim Reprod Sci 172:52–59. https://doi.org/10.1016/j.anireprosci.2016.07.002
Mascarenhas MN, Flaxman SR, Boerma T, Vanderpoel S, Stevens GA (2012) National, Regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLoS Med 9(12):1–12. https://doi.org/10.1371/journal.pmed.1001356
Masdiyasa IGS, Purnama IKE, Purnomo MH (2018) A new method to improve movement tracking of human sperms. IAENG Int J Comput Sci 45(4):114–122
Nagata MPB, Endo K, Ogata K, Yamanaka K, Egashira J, Katafuchi N, Yamashita K (2018) Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility. Proc Natl Acad Sci 115(14):201717974. https://doi.org/10.1073/pnas.1717974115
Nosrati R, Vollmer M, Eamer L, San Gabriel MC, Zeidan K, Zini A, Sinton D (2014) Rapid selection of sperm with high DNA integrity. Lab Chip 14(6):1142–1150. https://doi.org/10.1039/c3lc51254a
Nosrati R, Graham PJ, Zhang B, Riordon J, Lagunov A, Hannam TG, Sinton D (2017) Microfluidics for sperm analysis and selection. Nat Rev Urol 14(12):707–730. https://doi.org/10.1038/nrurol.2017.175
Park HW (2013) Effect of bioethics and safety act in medical research. J Korean Med Assoc 56(8):665–675. https://doi.org/10.5124/jkma.2013.56.8.665
Quinn MM, Jalalian L, Ribeiro S, Ona K, Demirci U, Cedars MI, Rosen MP (2018) Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples. Hum Reprod 33(8):1388–1393. https://doi.org/10.1093/humrep/dey239
Samuel R, Feng H, Jafek A, Despain D, Jenkins T, Gale B (2018) Microfluidic—based sperm sorting and analysis for treatment of male infertility. Transl Androl Urology 7(I):336–347. https://doi.org/10.21037/tau.2018.05.08
Tasoglu S, Safaee H, Zhang X, Kingsley JL, Catalano PN, Gurkan UA, Demirci U (2013) Exhaustion of racing sperm in nature-mimicking microfluidic channels during sorting. Small 9(20):3374–3384. https://doi.org/10.1002/smll.201300020
Tung C, Hu L, Fiore AG, Ardon F, Hickman DG, Gilbert RO, Wu M (2015) Microgrooves and fluid flows provide preferential passageways for sperm over pathogen Tritrichomonas foetus. Proc Natl Acad Sci 112(17):5431–5436. https://doi.org/10.1073/pnas.1500541112
US Food and Drug Administration (FDA) (2006) Guidance on informed consent for in vitro diagnostic device studies using leftover human specimens that are not individually identifiable. FDA Guidance Documents, FDA-2006-D-0150, pp 1–10
World Health Organization (2019) Sexual and reproductive health: infertility is a global public health issue. https://www.who.int/reproductivehealth/topics/infertility/perspective/en/. Accessed 30 May 2019
Xie L, Ma R, Han C, Su K, Zhang Q, Qiu T, Cheng J (2010) Integration of sperm motility and chemotaxis screening with a microchannel-based device. Clin Chem 56(8):1270–1278. https://doi.org/10.1373/clinchem.2010.146902
Yu S, Rubin M, Geevarughese S, Pino JS, Rodriguez HF, Asghar W (2018) Emerging technologies for home-based semen analysis. Andrology 6(1):10–19. https://doi.org/10.1111/andr.12441
Zhang X, Khimji I, Gurkan UA, Safaee H, Catalano PN, Keles HO, Demirci U (2011) Lensless imaging for simultaneous microfluidic sperm monitoring and sorting. Lab Chip 11(15):2535–2540. https://doi.org/10.1039/c1lc20236g
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This research was supported by the Korea Advanced Nano Fab Center (KANC) under Nano platform Research Grant.
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Kim, Y., Chun, K. New disposable microfluidic chip without evaporation effect for semen analysis in clinics and homes. Microsyst Technol 26, 647–655 (2020). https://doi.org/10.1007/s00542-019-04527-8
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DOI: https://doi.org/10.1007/s00542-019-04527-8