Zusammenfassung
Angeregt von dem volksmedizinischen Gebrauch von Papaya carica Blattmaterial zur Behandlung von Dengueinfektionen wurde eine Suspension von pulversisierten Carica papaya Blättern in Palmöl auf die Beeinflussung der Thrombozytenzahlen bei Mäusen untersucht. Jeweils 5 Mäuse erhielten peroral 15 mg der Carica papaya Blattsuspension, gleiche Volumina physiologischer Kochsalzlösung oder Palmöl. Die Thrombozytenzahl wurde unmittelbar vor sowie 1, 2, 4, 8, 10, 12, 24, 48 und 72 Stunden nach der Applikation bestimmt. Bei den mit C. papaya behandelten Mäusen zeigte sich eine signifikante Erhöhung der Thrombozytenzahl 1, 2, 4, 8, 10 und 12 Stunden nach der Anwendung. Die mit physiologischer Kochsalzlösung behandelte Gruppe zeigte lediglich eine zeitweilige, nicht signifikante Erhöhung der Thrombozytenzahlen, offenbar Ausdruck eines zirkadischen Zyklus. In der mit Palmöl allein behandelten Gruppe wurde eine protrahierte, bei 8 und 48 Stunden signifikante Erhöhung der Thrombozytenzahl verzeichnet, möglicherweise als Folge einer bisher unbekannten Stimulation der Freisetzung von Thrombozyten. Die Ergebnisse legen eine erweiterte Dosisstudie nahe, sowie die Isolierung und strukturelle Aufklärung der für die Ausschüttung bzw. Produktion von Thrombozyten verantwortlichen Inhaltsstoffe von C. papaya.
Summary
Following up a popular use of crude leaf preparations from Carica papaya for the treatment of dengue infections, a suspension of powdered Carica papaya leaves in palm oil has been investigated for its effect on thrombocyte counts in mice, administering by gavage 15 mg of powdered leaves per kg body weight to 5 mice. Equal numbers of animals received corresponding volumes of either palm oil alone or physiological saline solution. Thrombocyte counts before and at 1, 2, 4, 8, 10, 12, 24, 48 and 72 hours after dosing revealed significantly higher mean counts at 1, 2, 4, 8, 10 and 12 after dosing with the C. papaya leaf formulation as compared to the mean count at hour 0. There was only a non-significant rise of thrombocyte counts in the group having received saline solution, possibly the expression of a normal circadian rhythm in mice. The group having received palm oil only showed a protracted increase of platelet counts that was significant at hours 8 and 48 and obviously the result of a hitherto unknown stimulation of thrombocyte release. The results call for a dose-response investigation and for extending the studies to the isolation and identification of the C. papaya substances responsible for the release and/or production of thrombocytes.
References
Villegas VN (1997) Carica papaya L. In: Coronel RE, Verheij EWM (eds) Edible fruits and nuts. Wageningen University, The Netherlands, vol 1, p 447
Watson B (1997) Agronomy/agroclimatology notes for the production of papaya. MAFFA, Australia, p 187
Nakasone HY, Paull RE (1998) Tropical fruits. CAB International, Wallingford, UK, p 353
El Moussaoui A, Nijs M, Paul C, Wintjens R, Vincentelli J, Azarkan M, Looze Y (2001) Revisiting the enzymes stored in the lactifers of Carica papaya in the context of their possible participation in the plant defence mechanism. Cell Mol Life Sci 58: 556–70
Eno AE, Owo OI, Itam EH, Konya RS (2000) Blood pressure depression by the fruit juice of Carica papaya (L.) in renal and DOCA-induced hypertension in the rat. Phytother Res 14: 235–9
Wilson RK, Kwan TK, Kwan CY, Sorger GJ (2002) Effects of papaya seed extract and benzyl isothiocyanate on vascular contraction. Life Sci 71: 497–507
Emeruwa AC (1982) Antibacterial substance from Carica papaya fruit extract. J Nat Prod 45: 123–7
Rimbach G, Guo Q, Akiyama T, Matsugo S, Moini H, Virgili F, Packer L (2000) Ferric nitriloacetate induced DNA and protein damage: inhibitory effect of a fermented papaya preparation. Anticancer Res 20: 2907–14
Gupta OP, Sing S, Bani S, Sharma N, Malhotra S, Gupta BD, Banerjee SK, Handa SS (2000) Anti-inflammatory and anti-arthritic activities of silymarin acting through inhibition of 5-lipoxygenase. Phytomedicine 7: 21–4
Hewitt HH, Whittle S, Lopez SA, Bailey EY, Weaver SR (2000) Topical use of papaya in chronic skin ulcer therapy in Jamaica. West Indian Med J 49: 32–3
Manivannan B, Mishra PK, Pathak N, Sriram S, Bhande SS, Panneerdoss S, Lohiya NK (2004) Ultrastructural changes in the testis and epididymis of rats following treatment with the benzene chromatographic fraction of the chloroform extracts of the seed of Carica papaya. Phytother Res 18: 285–9
Udoh O, Essien I, Udoh F (2005) Effects of Carica papaya (paw paw) seeds extract on the morphology of pituitary-gonadal axis of male Wistar rats. Phytother Res 19: 1065–8
Chinoy NJ, D'Souza JM, Padman P (1994) Effects of crude aqueous extract of Carica papaya seeds in male albino mice. Reprod Toxicol 8: 75–9
Satrija F, Nansen P, Murtini S, He S (1995) Anthelminthic activity of papaya latex against patent Heligmosoides polygyrus infections in mice. J Ethnopharmacol 48: 161–4
Satrija F, Nansen P, Björn H, Murtini S, He S (1994) Effect of papaya latex against Ascaris suum in naturally infected pigs. J Helminthol 68: 343–6
World Health Organization (1997) Dengue haemorrhagic fever: diagnosis, treatment, prevention and control. 2nd edn. WHO, Geneva, p 31
Diehl KH, Hull R, Morton D, Pfister R, Rabemampianina Y, Smith D, Vidal JM, Vorstenbosch CVD (2001) A good practice guide to the administration of substances and removal of blood, including routes and volumes. J Appl Toxicol 21: 15–23
Lewis SM, Bain BJ, Bates I (eds) (2006) Dacie and Lewis practical haematology. Elsevier, UK, p 595
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sathasivam, K., Ramanathan, S., Mansor, S. et al. Thrombocyte counts in mice after the administration of papaya leaf suspension. Wien Klin Wochenschr 121 (Suppl 3), 19–22 (2009). https://doi.org/10.1007/s00508-009-1229-0
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00508-009-1229-0