Abstract
Key message
Cytokinin supplementation was essential for the induction of shoot proliferation and in vitro organogenesis from shoot apices and cotyledons of D. regia, respectively.
Abstract
Micropropagation techniques have been established for woody species to facilitate their mass clonal propagation and to better understand their processes of regeneration. The goal of this work was to evaluate the potential of shoot apex and cotyledonary explants of Delonix regia, commonly known as royal poinciana, to be induced to undergo shoot proliferation and in vitro organogenesis, respectively. Shoot apices and cotyledons obtained from in vitro-germinated seedlings were inoculated onto Murashige and Skoog (MS) medium supplemented with different concentrations (0.125, 0.250, 0.5, 1.0 and 2.0 mg L−1) of 6-benzyladenine (BA) and a control without BA. After 30 days of cultivation, multiple shoot formation and adventitious shoots were observed in shoot apices and cotyledonary segments of D. regia, respectively. Both explant sources exhibited a dose-dependent behavior with increased fresh mass and shoot number with increasing BA concentrations. The greatest number of shoots was observed in explants cultured on BA-supplemented (2.0 mg L−1) medium; however, the leaflets of shoots grown in MS medium with higher BA concentrations were smaller. Histological analysis revealed that the formation of adventitious shoots occurred through the reprogramming of mesophyll cells in the edge regions of the cotyledonary explants. The results obtained here provide new information for optimizing and establishing efficient regeneration systems and micropropagation of royal poinciana, an important ornamental tree species.
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References
Abdi G, Hedayat M (2011) Induction of somatic embryogenesis from immature zygotic embryo and immature seed of royal poinciana (Delonix regia). World Appl Sci J 13:391–395
Aqil F, Ahmad I, Mehmood Z (2006) Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish J Biol 30:177–183
Costa GM, Nepomuceno CF, Santana JRF (2010) Propagação in vitro de Erythrina velutina. Cienc Rural 40:1090–1096. https://doi.org/10.1590/S0103-84782010005000084
Couto JMF, Otoni WC, Pinheiro AL, Fonseca EP (2004) In vitro sterilization and germination of mahogany seeds (Swietenia macrophylla King). Rev Árvore 28:633–642. https://doi.org/10.1590/S0100-67622004000500002
Dias PC, Oliveira LS, Xavier A, Wendling I (2012) Cutting and mini-cutting techniques of Brazil wood species. Pesq Flor Bras 32:453–462. https://doi.org/10.4336/2012.pfb.32.72.453
Elhiti M, Stasolla C (2011) The use of zygotic embryos as explants for in vitro propagation: an overview. In: Thorpe TA, Yeung EC (eds) Plant embryo culture: methods and protocols, vol 710. Humana Press, New York, pp 229–255. https://doi.org/10.1007/978-1-61737-988-8_17
Gantait S, Kundu S, Das PK (2018) Acacia: an exclusive survey on in vitro propagation. J Saudi Soc Agric Sci 17:163–177. https://doi.org/10.1016/j.jssas.2016.03.004
Greenboim-Wainberg Y, Maymon I, Borochov R, Alvarez J, Olszewski N, Ori N, Eshed Y, Weiss D (2005) Cross talk between gibberellin and cytokinin: the Arabidopsis GA response inhibitor SPINDLY plays a positive role in cytokinin signaling. Plant Cell 17:92–102. https://doi.org/10.1105/tpc.104.028472
Gutiérrez IEM, Nepomuceno CF, Ledo CAS, Santana JRF (2011) In vitro regeneration of the Bauhinia cheilantha via organogenesis. Cienc Rural 41:260–265. https://doi.org/10.1590/S0103-84782011000200013
Ikeuchi M, Ogawa Y, Iwase A, Sugimoto K (2016) Plant regeneration: cellular origins and molecular mechanisms. Development 143:1442–1451. https://doi.org/10.1242/dev.134668
Lakshmanan P, Taji A (2000) Somatic embryogenesis in leguminous plants. Plant Biol 2:136–148. https://doi.org/10.1055/s-2000-9159
Lameira AO, Lopes SC, Nogueira RC, Cordeiro IMCC, Pinto JEBP, Reis LRS (2005) Effect of different concentrations of growth regulators on the mahogany (Swietenia macrophylla king) micropropagation through young explants. Plant Cell Cult Micropropag 1:53–58
Moura LC, Titon M, Fernandes JSC, Santana RC, Oliveira MLR (2012) Sucupira-preta micropropagation through axillary buds. Pesq agropec bras 47:1691–1698. https://doi.org/10.1590/S0100-204X2012001200003
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Myers AR, Vendrame WA (2004) Propagation of yellow kampong royal poinciana via somatic embryogenesis. Proc Fla State Hort Soc 117:338–341
Nunes EC, Castilho CV, Moreno FN, Viana AM (2002) In vitro culture of Cedrela fissilis Vellozo (Meliaceae). Plant Cell Tiss Org 70:259–268. https://doi.org/10.1023/A:1016509415222
Nunes EC, Laudano WLS, Moreno FN, Castilho CV, Mioto P, Sampaio FL, Bortoluzi JH, Benson EE, Pizolatti MG, Carasek E, Viana AM (2007) Micropropagation of Cedrela fissilis Vell. (Meliaceae). In: Jain SM, Häggman H (eds) Protocols for micropropagation of woody trees and fruits. Springer, Berlin, pp 221–235. https://doi.org/10.1007/978-1-4020-6352-7_21
O’Brien TP, McCully ME (1981) The study of plant structure: principles and selected methods. Termarcarphi, Melbourne
Oliveira LS, Dias PC, Brondani GE (2013) Micropropagation of Brazilian forest species. Pesq flor bras 33:439–453. https://doi.org/10.4336/2013.pfb.33.76.481
Parekh J, Chanda S (2007) Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. Afr J Biomed Res 10:175–181. https://doi.org/10.4314/ajbr.v10i2.50624
Rathore JS, Rathore V, Shekhawat NS, Singh RP, Liler G, Phulwaria M, Dagla HR (2004) Micropropagation of woody plants. In: Srivastava PS, Narula A, Srivastava S (eds) Plant biotechnology and molecular markers. Springer, Dordrecht, pp 195–205. https://doi.org/10.1007/1-4020-3213-7_13
Rocha DI, Monte-Bello CC, Dornelas MC (2015) Alternative induction of de novo shoot organogenesis or somatic embryogenesis from in vitro cultures of mature zygotic embryos of passion fruit (Passiflora edulis Sims) is modulated by the ratio between auxin and cytokinin in the medium. Plant Cell Tiss Org 120:1087–1098. https://doi.org/10.1007/s11240-014-0663-5
Shabir G, Anwar F, Sultana B, Khalid ZM, Afzal M, Khan QM, Ashrafuzzaman M (2011) Antioxidant and antimicrobial atributes and phenolics of different solvent extracts from leaves, flowers and bark of gold mohar [Delonix regia (Bojer ex Hook.) Raf]. Molecules 16:7302–7319. https://doi.org/10.3390/molecules16097302
Sharma SK, Millam S (2004) Somatic embryogenesis in Solanum tuberosum L.: a histological examination of key developmental stages. Plant Cell Rep 23:115–119. https://doi.org/10.1007/s00299-004-0814-y
Silva PP, Contim LAS, Freitas DV, Aride PHR, Santos ALW (2010) In vitro establishment of kapok tree (Ceiba pentandral. Gaertn) apical shoots. Sci Agrar 11:437–443. https://doi.org/10.5380/rsa.v11i6.20389
Singh S, Kumar SN (2014) A review: introduction to genus Delonix. J Pharm Pharm Sci 3:2042–2055
Sobrosa RC, Corder MPM (2003) Effect of the genotype on the potential for the production of shoots and adventitious roots in Eucalyptus grandis Hill ex Maiden in vitro. Floresta Ambient 10:58–68
Souza RR, Paiva PDO, Silva RR, Silva DPC, Reis MV, Paiva R (2019) Morphogenetic potential of different sources of explants for efficient in vitro regeneration of Genipa sp. Plant Cell Tissue Organ Cult 136:153–160. https://doi.org/10.1007/s11240-018-1501-y
Su YH, Liu YB, Zhang XS (2011) Auxin-cytokinin interaction regulates meristem development. Mol Plant 4:616–625. https://doi.org/10.1093/mp/ssr007
Torres AC, Teixeira SL, Pozzer L (1998) Cultura de ápices caulinares e recuperação de plantas livres de vírus. In: Torres AC, Caldas LS, Buso JA (eds) Cultura de tecidos e transformação genética de plantas. Embrapa, Brasília, pp 133–145
Varshney A, Anis M (2014) Trees: propagation and conservation, biotechnological approaches for the propagation of a multipurpose tree, Balanites aegyptica Del, vol XVI. Springer, Berlin. https://doi.org/10.1007/978-81-322-1701-5
Wang XD, Nolan KE, Irwanto RR, Sheahan MB, Rose RJ (2011) Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells. Ann Bot 107:599–609. https://doi.org/10.1093/aob/mcq269
Wendling I, Dutra LF, Grossi F (2006) Produção de mudas de espécies lenhosas. Embrapa Florestas, Colombo
Werner ET, Cuzzuo GRF, Pessotti KV, Lopes FP, Roger JA (2009) In vitro calogenesis control of pau-brasil. Rev Árvore 33:987–996. https://doi.org/10.1590/S0100-67622009000600001
Winkelmann T (2013) Recent advances in the propagation of woody plants. Acta Hortic 990:375–381. https://doi.org/10.17660/ActaHortic.2013.990.47
Yadav R, Yadav N, Kumar S (2016) An improved micropropagation and assessment of genetic fidelity in multipurpose medicinal tree, Acacia auriculiformis. Proc Natl Acad Sci India Sect B Biol Sci 86:921–929. https://doi.org/10.1007/s40011-015-0550-9
Zimmermann FJP (2004) Estatística aplicada à pesquisa agrícola. Embrapa Arroz e Feijão, Santo Antônio de Goiás city
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This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—Finance Code 001 and the Coordenação de Pesquisa e Inovação Tecnológica—Federal University of Goiás (COPI / UFG).
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de Oliveira Costa, A., Silva, L.A.S., Duarte, I.M. et al. Shoot proliferation and in vitro organogenesis from shoot apex and cotyledonary explants of royal poinciana (Delonix regia), an ornamental leguminous tree. Trees 34, 189–197 (2020). https://doi.org/10.1007/s00468-019-01910-w
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DOI: https://doi.org/10.1007/s00468-019-01910-w