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Molecular cloning, characterization, and functional analysis of CcBBM gene from camphor tree (Cinnamomum camphora L.)

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Abstract

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Cloning and functional analysis of CcBBM gene.

Abstract

Baby boom (BBM), as a transcription factor of AP2/ERF family, has great potence in promoting somatic embryogenesis. In this study, a BBM gene from somatic embryos of camphor tree (Cinnamomum camphora L.), named CcBBM, was cloned and characterized. A partial fragment of 560 bp of CcBBM was amplified by degenerate PCR based on the two conserved AP2 domains of other annotated BBMs. Full-length cDNA of CcBBM was obtained by a cloning strategy combing degenerate PCR and RACE technique. The open-reading frame of CcBBM is 2169 bp in length, which encodes 722 amino acids. A comparison of the deduced amino acid sequence of CcBBM with BBM from other species showed high similarities, ranging from 51 to 80 %. The mRNA levels of CcBBM in different tissues were detected by semi-quantitative reverse transcription PCR (RT-PCR). CcBBM was expressed in most tissues, with highest expression level in somatic embryos, followed by roots, young fruits and flowers. Ectopic expression of CcBBM gene in Arabidopsis exhibited a variety of deformed and variegated leaves and short roots. No spontaneous somatic embryogenesis was observed in 35S::CcBBM transgenic plants. These results will help us understand the role of CcBBM in somatic embryogenesis in camphor tree.

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Acknowledgments

This study is funded by the National Natural Science Foundation of China (31100508), and the Ministry of Education of China (IRT13065).

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    Authors and Affiliations

    1. Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

      Qinhong Liu, Cuijie Zhang, Yihui Cheng, Bo Zheng, Manzhu Bao & Xueping Shi

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    1. Qinhong Liu
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    2. Cuijie Zhang
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    3. Yihui Cheng
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    4. Bo Zheng
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    Correspondence to Xueping Shi.

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    Communicated by J. Carlson.

    Qinhong Liu and Cuijie Zhang contributed equally.

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    Liu, Q., Zhang, C., Cheng, Y. et al. Molecular cloning, characterization, and functional analysis of CcBBM gene from camphor tree (Cinnamomum camphora L.). Trees 30, 1033–1043 (2016). https://doi.org/10.1007/s00468-015-1339-4

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    • DOI: https://doi.org/10.1007/s00468-015-1339-4

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