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Developmental disorders in buds and needles of mature Norway spruce, Picea abies (L.) Karst., in relation to needle boron concentrations

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Abstract

Dieback of the terminal shoot and consequently bushy growth induced by boron deficiency have been reported widely throughout the world in several tree species. Recently, similar growth damage was documented in half of the young spruce stands in eastern Finland. To clarify the role of B deficiency, the light microscopic structure of emerging buds and of developing and previous-year needles of mature Norway spruce (Picea abies L. Karst.) from damaged (D stand), partly damaged (PD stand) and healthy (H stand) stands were analysed. The samples, on which needle nutrient concentrations were also determined, were taken seven times between early spring (April) and early winter (November). Cell death characterized by precipitation of the cell content, possibly due to the release of tannins after membrane rupture, was seen in the apex of emerging buds, and this led to fatal damage in about half of the buds in the trees from the D stand, where the needle B concentration was well below the deficiency level of 4–5 mg kg−1. Furthermore, an increase in living cells that accumulated tannins in the vacuoles, which is a common stress and/or defense reaction, was found in the primordial shoots of buds and in the differentiating needles in the PD and D stands. The increase in the areas of the central cylinder and of the xylem found in the needles indicate structural plasticity during needle differentiation to drought. The time frame for bud emergence from late May up to mid-September means that an adequate B supply is necessary throughout the summer in order to avoid fatal bud damage and thus bushy growth of the trees.

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References

  • Anglberger H, Sieghardt M, Katzensteiner K, Halmschlager E (2003) Needle nutrient status of Sirococcus shoot blight-diseased and healthy Norway spruces. For Path 33:21–29

    Google Scholar 

  • Aronsson A (1983) Growth disturbances caused by boron deficiency in some fertilized pine and spruce stands on mineral soils. Commun Inst For Fenn 116:116–122

    Google Scholar 

  • Bílková J, Albrechtová J, Opatrná J (1999) Histochemical detection and image analysis of non-specific esterase activity and the amount of polyphenols during annual bud development in Norway spruce. J Exp Bot 35:1129–1138

    Article  Google Scholar 

  • Braekke FH (1983) Occurrence of growth disturbance problems in Norwegian and Swedish forestry. Commun Inst For Fenn 116:20–25

    Google Scholar 

  • Braekke FH (1994) Diagnostiske grenseverdier for naeringselementer i gran- og furunåler. Aktuelt fra Skogforsk 15:11 (In Norwegian)

    Google Scholar 

  • Cajander AK (1949) Forest types and their significance. Acta Forestalia Fennica 56:69

    Google Scholar 

  • Cakmak I, Kurz H, Marschner H (1995) Short-term effects of boron, germanium, and high light intensity on membrane permeability in boron deficient leaves of sunflower. Physiol Plant 95:11–18

    Article  CAS  Google Scholar 

  • Cakmak I, Römheld V (1997) Boron deficiency-induced impairments of cellular functions in plants. Plant Soil 193:71–83

    Article  CAS  Google Scholar 

  • Cannel MGR, Willet SC (1975) Rates and times at which needles are initiated in buds on differing provenances of Pinus contorta and Picea sitchensis in Scotland. Can J For Res 5:367–380

    Article  Google Scholar 

  • Carter RE Brockley RP (1990) Boron deficiencies in British Columbia: diagnosis and treatment evaluation. For Ecol Manage 37:83–94

    Article  Google Scholar 

  • Correia MJ, Coelho D, David MM (2001) Response to seasonal drought in three cultivars of Ceratonia siliqua: leaf growth and water relations. Tree Physiol 21:645–653

    PubMed  CAS  Google Scholar 

  • de Faÿ E, Vacher V, Humbert F (2000) Water-related phenomena in winter buds and twigs of Picea abies L. (Karst.) until bud-burst: a biological, histological and NMR study. Ann Bot 86:1097–1107

    Article  Google Scholar 

  • Dell B, Huang L (1997) Physiological response of plants to low boron. Plant Soil 193:103–120

    Article  CAS  Google Scholar 

  • Deslauriers A, Morin H (2005) Intra-annual tracheid production in balsam fir stem and the effect of meteorological variables. Trees 19:402–408

    Article  Google Scholar 

  • Dordas C, Brown PH (2005) Boron deficiency affects cell viability, phenolic leakage and oxidative burst in rose cell cultures. Plant Soil 268:293–301

    Article  CAS  Google Scholar 

  • Fink S (1999) Pathological and regenerative plant anatomy. Berlin: Gebruder Bornträger

  • Gilmore DW, Seymour RS, Halteman WA, Greenwood MS (1995) Canopy dynamics and the morphological development of Abies balsamea: effects of foliage age on specific leaf area and secondary vascular development. Tree Physiol 15:47–55

    PubMed  Google Scholar 

  • Goldberg S (1997) Reactions of boron with soils. Plant Soil 193:35–48

    Article  CAS  Google Scholar 

  • Halmschlager E, Gabler A, Andrae F (2000) The impact of Sirococcus shoot blight on radial and height growth of Norway spruce (Picea abies) in young plantations. For Path 30:127–133

    Article  Google Scholar 

  • Halonen O, Tulkki H, Derome J (1983) Nutrient analysis methods. Metsäntutkimuslaitoksen tiedonantoja 121:28

    Google Scholar 

  • Heikinheimo O (1915) Kaskiviljelyksen vaikutus Suomen metsiin. Acta Forestalia Fennica 4(2):264

    Google Scholar 

  • Holopainen T, Anttonen S, Wulff A, Palomäki V, Kärenlampi L (1992) Comparative evaluation of the effects of gaseous pollutants, acidic deposition and mineral deficiencies: structural changes in the cells of forest plants. Agric Ecosyst Environ 42:365–398

    Article  CAS  Google Scholar 

  • Jäntti A (1947) Suomen laidunolot. Referat: Die Weideverhältnisse in Finnland. Acta Forestalia Fennica 53:254

    Google Scholar 

  • Järvinen E, Hokkanen TJ, Kuuluvainen T (1993) Spatial heterogeneity and relationships of mineral soil properties in boreal Pinys sylvestris stand. Scand J For Res 8:435–445

    Article  Google Scholar 

  • Jokela A, Bäck J, Huttunen S, Jalkanen R (1995) Excess nitrogen fertilization and the structure of Scots-pine needles. Eur J For Path 25:109–124

    Article  Google Scholar 

  • Jukka L (1988) Metsänterveysopas. Metsätuhot ja niiden torjunta. Samreka Oy, Helsinki, 168 p

    Google Scholar 

  • Liakopoulos G, Karabourniotis G (2005) Boron deficiency and concentrations and composition of phenolic compounds in Olea europaea leaves: a combined growth chamber and field study. Tree Physiol 25:307–315

    PubMed  CAS  Google Scholar 

  • Lilja S (1967) Tuomen merkityksestä kuusen tuomiruostesienen, Pucciniastrum padi (Kunze & Schum.) Diet. esiintymiselle kuusessa. Summary: On the occurrence of the bird cherie (Prunus padus L.) for the occurrence of the rust Puccniastrum padi (Kunze & Schum.) Diet. in spruce. Silva Fennica 1:45–62

    Google Scholar 

  • Linder S (1995) Foliar analysis for detecting and correcting nutrient imbalances in Norway spruce. Ecol Bull (Copenhagen) 44:178–190

    CAS  Google Scholar 

  • Loomis WD, Durst RW (1992) Chemistry and biology of boron. BioFactors 3:229–239

    PubMed  CAS  Google Scholar 

  • Matoh T (1997) Boron in plant cell walls. Plant Soil 193:59–70

    Article  CAS  Google Scholar 

  • Möttönen M, Järvinen E, Hokkanen TJ, Kuuluvainen T, Ohtonen R (1999) Spatial distribution of soil ergosterol in the organic layer of a mature Scots pine (Pinus sylvestris L.) forest. Soil Biol Biochem 31:503–516

    Article  Google Scholar 

  • Möttonen M, Lehto T, Aphalo PJ (2001) Growth dynamics and mycorrhizas of Norway spruce (Picea abies) seedlings in relation to boron supply. Trees 15:319–326

    Article  Google Scholar 

  • Power PP, Woods WG (1997) The chemistry of boron and its specification in plants. Plant Soil 193:1–13

    Article  CAS  Google Scholar 

  • Raitio H (1983) Macro- and microscopic symptoms in growth disturbed forest trees. Commun Inst For Fenn 116:35–39

    Google Scholar 

  • Raitio H, Merilä P (1998) Seasonal variation in the size and chemical composition of Scots pine and Norway spruce needles in different conditions. Technical Report, European Programme for the Intensive Monitoring of Forest Ecosystems/Level II, Finland, The Finnish Forest Research Institute, Parkano Research Station, 44 p.

  • Raulo J, Leikola M (1974) Tutkimuksia puiden vuotuisen pituuskasvun ajoittumisesta. Summary: Studies on the annual height growth of trees. Commun Inst For Fenn 81(2):19

    Google Scholar 

  • Rehfuess K-E, Bosch C, Pfannkuch E (1983) Nutrient imbalances in coniferous stands in southern Germany. Commun Inst For Fenn 116:122–130

    Google Scholar 

  • Rerkasem B, Jamjod S (1997) Genotypic variation in plant response to low boron and implications for plant breeding. Plant Soil 193:169–180

    Article  CAS  Google Scholar 

  • Richardson AD, Ashton PMS, Berlyn GP, Mcgroddy ME, Cameron IR (2001) Within-crown foliar plasticity of western hemlock, Tsuga heterophylla, in relation to stand age. Ann Bot 88:1007–1015

    Article  Google Scholar 

  • Ryyppö A, Sutinen S, Mäenpää M, Vapaavuori E, Repo T (1997) Frost damage and recovery of Scots pine seedlings at the end of the growing season. Can J For Res 27:1376–1382

    Article  Google Scholar 

  • Saalas U (1949) Suomen metsähyönteiset sekä muut metsälle vahingolliset ja hyödylliset eläimet. Suomelainen tiedeakatemia. Suomen tiedettä no 5, WSOY, Helsinki, 719 p (In Finnish)

  • Saarsalmi A, Tamminen P (2005) Boron, phosphorus and nitrogen fertilization in Norway spruce stands suffering from growth disturbances. Silva Fennica 39(3):351–364

    Google Scholar 

  • Silfverberg K (1980) Kuusen kasvuhäiriö ja hivenravinteet. Abstract: Micronutrional disorder in Norway spruce. Folia For 432:13

    Google Scholar 

  • Soikkeli S (1980) Ultrastructure of the mesophyll in Scots pine and Norway spruce: seasonal variation and molarity of fixative buffer. Protoplasma 103:241–252

    Article  Google Scholar 

  • Stavrianakou S, Liakopoulos G, Karabourniotis G (2006) Boron deficiency on growth, photosynthesis and relative concentrations of phenolics of Dittrichia viscose (Asteraceae). Environ Exp Bot 56:293–300

    Google Scholar 

  • Stone EL (1990) Boron deficiency and excess in forest trees: a review. For Ecol Manage 37:49–75

    Article  Google Scholar 

  • Sutinen S, Koivisto L (1995) Microscopic structure of conifer needles as a diagnostic tool in the field. In: Munawar M, Hänninen O, Roy S, Munawar N, Kärenlampi L, Brown D (eds) Bioindicators of environmental health. Ecovision world monograph series. SPB Academic Publishing, Amsterdam, The Netherlands, pp 73–81

    Google Scholar 

  • Svobodová H, Lipavská H, Albrechtová J (2000) Non-structural carbohydrate status in Norway spruce buds in the context of annual bud structural development as affected by acidic pollution. Environ Exp Bot 43:253–265

    Article  PubMed  Google Scholar 

  • Tamminen P (1991) Kangasmaan ravinnetunnusten ilmaiseminen ja viljavuuden alueellinen vaihtelu Etelä-Suomessa. Summary: Expression of soil nutrient status and regional variation in soil fertility of forested sites in southern Finland, FF 777, 40 p

  • Tamminen P, Saarsalmi A (2004) Viljavien maiden nuorten kuusikoiden neulasten booripitoisuus Etelä-Suomessa. Metsätieteen aikakauskirja 3/2004:271–283 (In Finnish)

    Google Scholar 

  • Wikner B (1983) Distribution and mobility of boron in forest ecosystems. Commun Inst For Fenn 116:131–141

    Google Scholar 

Download references

Acknowledgements

We thank Marja-Leena Jalkanen, Osmo Korhonen and Seija Repo for their assistance in the field and in the laboratory.

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Authors and Affiliations

  1. Joensuu Research Unit, Finnish Forest Research Institute, P.O. Box 68, 80101, Joensuu, Finland

    Sirkka Sutinen, Martti Vuorinen & Risto Rikala

  2. Suonenjoki Research Unit, Finnish Forest Research Institute, 77600, Suonenjoki, Finland

    Sirkka Sutinen, Martti Vuorinen & Risto Rikala

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  1. Sirkka Sutinen
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  2. Martti Vuorinen
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  3. Risto Rikala
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Correspondence to Sirkka Sutinen.

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Communicated by H. Rennenberg

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Sutinen, S., Vuorinen, M. & Rikala, R. Developmental disorders in buds and needles of mature Norway spruce, Picea abies (L.) Karst., in relation to needle boron concentrations. Trees 20, 559–570 (2006). https://doi.org/10.1007/s00468-006-0071-5

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  • DOI: https://doi.org/10.1007/s00468-006-0071-5

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