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Saccharomyces boulardii prevents oral-poliovirus vaccine-induced IgA nephropathy in mice

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Abstract

Immunoglobulin A nephropathy (IgAN) is associated with mucosal IgA defect. Probiotics regulate specific and innate immunity. We evaluated the effect of Saccharomyces boulardii on experimental IgAN in mice. Four groups of BALB/c mice (eight for each) were formed. Group 1 was immunized by oral poliovirus vaccine (OPV) at 0, 14, and 28 days. Group 2 was also given S. boulardii in addition to OPV. Group 3 was given only S. boulardii, whereas group 4 received no treatment. At week 6, after urine and serum samples were obtained for urinalysis and serum creatinine and IgA measurements, all animals were sacrificed to get their kidneys for histopathological evaluation. Urinalysis and serum creatinine levels were normal in all groups. Serum IgA level was increased only in group 1. Whereas group 1 had mesangial proliferation, histology was normal in the other groups. Predominant IgA deposition was universal in group 1, whereas it was either not present or minimal in other groups. Three mice in group 1 also had C3 deposition, which was absent in other groups. Electron microscopy revealed mesangial proliferation, matrix expansion, focal glomerular basement membrane thickening and electron-dense deposits in group 1 only, whereas the other groups were normal. In conclusion, enteral S. boulardii prevented OPV-induced IgAN in mice.

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Acknowledgement

We thank Izmir Branch of the Turkish Society of Nephrology for their financial contribution to the work.

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Authors and Affiliations

  1. Department of Pediatrics, Dokuz Eylül University Medical Faculty, Balcova 35340, Izmir, Turkey

    Alper Soylu, Sema Berktaş, Belde K. Demir, Didem Yeşilırmak, Mehmet Türkmen & Salih Kavukçu

  2. Department of Pathology, Dokuz Eylül University Medical Faculty, Balcova 35340, Izmir, Turkey

    Sülen Sarıoğlu & Yahya Tufan

  3. Department of Histology and Embryology, Dokuz Eylül University Medical Faculty, Balcova 35340, Izmir, Turkey

    Güven Erbil

  4. Department of Physiology, Dokuz Eylül University Medical Faculty, Balcova 35340, Izmir, Turkey

    Osman Yılmaz

  5. Çamlıçay Mah. Özgür Sitesi, No:12/A Urla, İzmir, Turkey

    Alper Soylu

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  1. Alper Soylu
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  2. Sema Berktaş
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  9. Mehmet Türkmen
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  10. Salih Kavukçu
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Correspondence to Alper Soylu.

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Soylu, A., Berktaş, S., Sarıoğlu, S. et al. Saccharomyces boulardii prevents oral-poliovirus vaccine-induced IgA nephropathy in mice. Pediatr Nephrol 23, 1287–1291 (2008). https://doi.org/10.1007/s00467-008-0813-2

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  • DOI: https://doi.org/10.1007/s00467-008-0813-2

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