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Endothelial nitric oxide synthase gene intron 4 a/b VNTR polymorphism in children with APSGN

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Abstract

The role of endothelial nitric oxide synthase gene intron 4 a/b (eNOS4a/b) variable number of tandem repeats (VNTR) polymorphism in various renal diseases was investigated. We investigated whether the eNOS4a/b VNTR polymorphism is associated with susceptibility to acute poststreptococcal glomerulonephritis (APSGN) and its clinical features. Endothelial NOS4a/b VNTR polymorphism is determined by the polymerase chain reaction in 60 children with APSGN, and 66 healthy controls. The genotype distribution of eNOS4 does not differ between the patients and the controls (X2=5.1, p=0.079). However, the frequency of eNOS4a (eNOS4a/a and eNOS4a/b) genotype is higher in the patients than in the controls (X2=4.5, p=0.046). In the APSGN group we performed renal biopsy on eight patients because of nephrotic syndrome accompanies acute nephritic syndrome or glomerular filtration rate (GFR) is lower than 50% of normal, and found that to carry a/a and a/b genotypes were a significant risk factor for this type presentation (OR=17.3, 95% CI:1.95-152.67, p=0.03). Mean serum creatinine values are found statistically significantly higher in a/a and a/b genotypes when compared with b/b genotypes (p=0.022). Children carrying the “aa” and “ab” genotype or “a” allele of eNOS4 have a greater tendency to develop and clinical presentation of APSGN.

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Acknowledgements

This study was supported by the Cukurova University Research Foundation (Nr. TF2003BAP-25). We would also like to thank to Halil Gulsev for his help with the laboratory procedures and our PD nurse Gulsen Kaygusuz.

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Authors and Affiliations

  1. Pediatric Nephrology, Cukurova University, Balcali Adana, Yuregir, 01330, Turkey

    Hasan Dursun, Aytul Noyan, Mithat Buyukcelik, Mustafa Soran, Aysun K. Bayazit & Ali Anarat

  2. Biochemistry, Cukurova University, Balcali Adana, Yuregir, 01330, Turkey

    Selcuk Matyar & Gulen Attila

  3. Pediatric Nephrology, Baskent University Adana Research Hospital, Balcali Adana, Yuregir, 01330, Turkey

    Nurcan Cengiz

  4. Biostatistics, Cukurova University, Balcali Adana, Yuregir, 01330, Turkey

    Gulsah Seydaoglu

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  1. Hasan Dursun
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  2. Aytul Noyan
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Correspondence to Hasan Dursun.

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Dursun, H., Noyan, A., Matyar, S. et al. Endothelial nitric oxide synthase gene intron 4 a/b VNTR polymorphism in children with APSGN. Pediatr Nephrol 21, 1661–1665 (2006). https://doi.org/10.1007/s00467-006-0202-7

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  • DOI: https://doi.org/10.1007/s00467-006-0202-7

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