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Production of butanol from distillers’ grain waste by a new aerotolerant strain of Clostridium beijerinckii LY-5

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Abstract

A new aerotolerant strain of Clostridium beijerinckii LY-5 was isolated from the pit mud of the Chinese Baijiu-making process for butanol production. Plackett–Burman design and artificial neural network were used to optimize the fermentation medium and a total of 13.54 ± 0.22 g/L butanol and 19.91 ± 0.52 g/L ABE were attained under aerotolerant condition. Moreover, distillers’ grain waste (DGW), the main by-product in the Baijiu production process, was utilized as potential substrate for butanol production. DGW was hydrolyzed by α-amylase and glucoamylase and then fermented after a detoxifying process of overliming. Butanol and ABE concentrations were 9.02 ± 0.18 and 9.57 ± 0.19 g/L with the yield of 0.21 and 0.23 g/g sugar, respectively. The higher ratio of butanol to ABE might be caused by the inhibitors in DGW medium affecting the metabolic pathways of C. beijerinckii LY-5 and approximately 1.48 ± 0.04 g/L isopropanol was found at the end of fermentation. This work highlights the feasibility of using DGW as a promising feedstock for butanol production by a new aerotolerant strain of C. beijerinckii LY-5, with benefit to the environment.

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Acknowledgements

This work was supported by the Project of Central Government Guidance of Local Scientific and Technological Development (2019ZYYD025), the Research Project of Hubei Provincial Department of Education (Q20191408), and the Scientific Research Foundation of Hubei University of Technology (No. [2019]10). We thank the Bishengquan liquor industry Co. Ltd., China for their support and anonymous reviewers for critically evaluating this manuscript.

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Authors and Affiliations

  1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, School of Food and Biological Engineering, Hubei University of Technology, 28 Nanli Road, Wuhan, 430068, China

    Jiang-Bo Wang, Bo Kong, Hao Wang, Lin-Yang Cai, Rui-Jing Zhang, Feng-Jiao Cai, Zheng-Jun Zhu, Jing-Hua Cao & Jian Xu

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  1. Jiang-Bo Wang
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Correspondence to Jian Xu.

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Wang, JB., Kong, B., Wang, H. et al. Production of butanol from distillers’ grain waste by a new aerotolerant strain of Clostridium beijerinckii LY-5. Bioprocess Biosyst Eng 44, 2167–2179 (2021). https://doi.org/10.1007/s00449-021-02592-w

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