Abstract
Coffee husk (CH), a waste obtained from processing of coffee cherries via dry method, causes serious environmental problems. In this study, strategies were designed to utilize CH for succinic acid (SA) production. Three different CH hydrolysis methods: thermal, thermochemical and crude enzymes obtained by solid state fermentation of Aspergillus niger and Trichoderma reesei, were evaluated to generate fermentable feedstock for SA production using Actinobacillus succinogenes. The feasibility of these pretreatment methods was investigated. Accordingly, thermochemical hydrolysis using H2SO4 at 121 °C for 30 min, appeared the most effective method for CH hydrolysis, producing 24.4 g/L of reducing sugars (RS). Finally, 19.3 g/L of SA with yield and productivity of 0.95 g SA/g RS and 0.54 g/L/h, respectively, were obtained using CH hydrolysate. The current study revealed an alternative way of utilization coffee waste for value addition while mitigating environmental problems caused by its disposal.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Nos. 21706125, 21727818, 21706124, 31700092), the Key Science and Technology Project of Jiangsu Province (BE2016389), the Project of State Key Laboratory of Materials-Oriented Chemical Engineering (KL16-08), and Top-notch Academic Programs Project of Jiangsu Higher Education Institutions PPZY2015B155, TAPP.
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Dessie, W., Zhu, J., Xin, F. et al. Bio-succinic acid production from coffee husk treated with thermochemical and fungal hydrolysis. Bioprocess Biosyst Eng 41, 1461–1470 (2018). https://doi.org/10.1007/s00449-018-1974-4
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DOI: https://doi.org/10.1007/s00449-018-1974-4