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Use of extracts from oyster shell and soil for cultivation of Spirulina maxima

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Abstract

Calcium ion and trace metals play important roles in various metabolisms of photosynthetic organisms. In this study, simple methods were developed to extract calcium ion and micronutrients from oyster shell and common soil, and the prepared extracts were tested as a replacement of the corresponding chemicals that are essential for growth of microalgae. The oyster shell and soil were treated with 0.1 M sodium hydroxide or with 10 % hydrogen peroxide, respectively. The potential application of these natural sources to cultivation was investigated with Spirulina maxima. When compared to standard Zarrouk medium, the Spirulina maxima cultivated in a modified Zarrouk media with elements from oyster shell and soil extract exhibited increases in biomass, chlorophyll, and phycocyanin by 17, 16, and 64 %, respectively. These results indicate that the extracts of oyster shell and soil provide sufficient amounts of calcium and trace metals for successful cultivation of Spirulina maxima.

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Abbreviations

AOAC:

Association of Analytical Community

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Acknowledgments

This work was supported by the Advanced Biomass R&D Center (ABC) of Korea with a Grant from the Ministry of Science, ICT and Future Planning (ABC-2010-0029728). Special thanks go to Korea Institute of Ocean Science and Technology for cooperation.

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Authors and Affiliations

  1. Advanced Biomass R&D Center, KAIST, 291 Daehakno, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Joo-Young Jung, Min S. Park & Ji-Won Yang

  2. Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehakno, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Sunmin Kim, Hansol Lee, Kyochan Kim, Woong Kim, Min S. Park, Jong-Hee Kwon & Ji-Won Yang

  3. Global Bioresources Research Center, Korea Institute of Ocean Science & Technology, Ansan, 426-744, Republic of Korea

    Joo-Young Jung

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  1. Joo-Young Jung
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  2. Sunmin Kim
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  3. Hansol Lee
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  4. Kyochan Kim
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  5. Woong Kim
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  6. Min S. Park
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  7. Jong-Hee Kwon
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Correspondence to Jong-Hee Kwon or Ji-Won Yang.

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Jung, JY., Kim, S., Lee, H. et al. Use of extracts from oyster shell and soil for cultivation of Spirulina maxima . Bioprocess Biosyst Eng 37, 2395–2400 (2014). https://doi.org/10.1007/s00449-014-1216-3

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  • DOI: https://doi.org/10.1007/s00449-014-1216-3

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