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M13 bacteriophage production for large-scale applications

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Abstract

Bacteriophage materials have the potential to revolutionize medicine, energy production and storage, agriculture, solar cells, optics and many other fields. To fulfill these needs, this study examined critical process parameters during phage propagation to increase phage production capability. A representative scale-down system was created in tube spin reactors to allow parallel experimentation with single- and multi-variable analysis. Temperature, harvest time, media composition, feed regime, bacteriophage, and bacteria concentration were analyzed in the scale-down system. Temperature, media composition, and feeding regimens were found to affect phage production more than other factors. Temperature affected bacterial growth and phage production inversely. Multi-variate analysis identified an optimal parameter space which provided a significant improvement over the base line method. This method should be useful in scaled production of bacteriophage for biotechnology.

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Authors and Affiliations

  1. Environmental Laboratory, US Army Engineer Research and Development Center, 3909 Halls Ferry Road, Vicksburg, MS, 39180, USA

    Christopher M. Warner, Natalie Barker & Edward J. Perkins

  2. Department of Bioengineering, University of California, Berkeley, CA, 94720, USA

    Seung-Wuk Lee

  3. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA

    Seung-Wuk Lee

Authors
  1. Christopher M. Warner
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  2. Natalie Barker
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  3. Seung-Wuk Lee
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  4. Edward J. Perkins
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Correspondence to Christopher M. Warner.

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Warner, C.M., Barker, N., Lee, SW. et al. M13 bacteriophage production for large-scale applications. Bioprocess Biosyst Eng 37, 2067–2072 (2014). https://doi.org/10.1007/s00449-014-1184-7

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  • DOI: https://doi.org/10.1007/s00449-014-1184-7

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