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Screening of Ganoderma strains with high polysaccharides and ganoderic acid contents and optimization of the fermentation medium by statistical methods

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Abstract

Polysaccharides and ganoderic acids (GAs) are the major bioactive constituents of Ganoderma species. However, the commercialization of their production was limited by low yield in the submerged culture of Ganoderma despite improvement made in recent years. In this work, twelve Ganoderma strains were screened to efficiently produce polysaccharides and GAs, and Ganoderma lucidum 5.26 (GL 5.26) that had been never reported in fermentation process was found to be most efficient among the tested stains. Then, the fermentation medium was optimized for GL 5.26 by statistical method. Firstly, glucose and yeast extract were found to be the optimum carbon source and nitrogen source according to the single-factor tests. Ferric sulfate was found to have significant effect on GL 5.26 biomass production according to the results of Plackett–Burman design. The concentrations of glucose, yeast extract and ferric sulfate were further optimized by response surface methodology. The optimum medium composition was 55 g/L of glucose, 14 g/L of yeast extract, 0.3 g/L of ferric acid, with other medium components unchanged. The optimized medium was testified in the 10-L bioreactor, and the production of biomass, IPS, total GAs and GA-T enhanced by 85, 27, 49 and 93 %, respectively, compared to the initial medium. The fermentation process was scaled up to 300-L bioreactor; it showed good IPS (3.6 g/L) and GAs (670 mg/L) production. The biomass was 23.9 g/L in 300-L bioreactor, which was the highest biomass production in pilot scale. According to this study, the strain GL 5.26 showed good fermentation property by optimizing the medium. It might be a candidate industrial strain by further process optimization and scale-up study.

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Acknowledgments

This work was supported by post-doctoral station of Hangzhou Wahaha Group Co., Ltd.

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Food Biotechnology, Institute of Bioengineering, Hangzhou Wahaha Group Co., Ltd., Hangzhou, 310018, China

    Zhen-hua Wei, Ying-yi Duan, Yong-qing Qian, Xiao-feng Guo, Yan-jun Li, Shi-he Jin, Sheng-yan Shan, Chun-ru Wang & Xue-jiao Chen

  2. State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China

    Zhen-hua Wei & Zhong-Xin Zhou

  3. Department of Bioengineering, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014, China

    Yuguo Zheng

  4. State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Jian-Jiang Zhong

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  1. Zhen-hua Wei
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Correspondence to Zhen-hua Wei.

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Wei, Zh., Duan, Yy., Qian, Yq. et al. Screening of Ganoderma strains with high polysaccharides and ganoderic acid contents and optimization of the fermentation medium by statistical methods. Bioprocess Biosyst Eng 37, 1789–1797 (2014). https://doi.org/10.1007/s00449-014-1152-2

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  • DOI: https://doi.org/10.1007/s00449-014-1152-2

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