Skip to main content
SpringerLink
Log in

Effects of ultrasonic and microwave pretreatments on lipid extraction of microalgae

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

The extraction of lipids from microalgal cells using ultrasonic and microwave pretreatments is mechanistically evaluated based on the distribution of cell fragments, the lipid content analysis, the scanning electron microscopic (SEM) observation of ruptured microalgal cells, and the analysis of fatty acids. The results indicate that microwave pretreatment extracts lipids more rapidly and efficiently as compared to ultrasonic pretreatment. The rupture of cells in the microwave process is due to the tremendous pressure caused by the rapid heating of the moisture inside the microalgal cells, whereas in the ultrasonic process the microalgal cells are ruptured by shock waves from cavitation bubbles outside the cells. The fatty acid composition of the respective lipids extracted via the two types of pretreatment did not vary significantly from one another. These results demonstrate that the microwave process is rapid and more effective than the ultrasonic process for lipid extraction from microalgae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Sawayama S, Inoue S, Dote Y, Yokoyama SY (1995) CO2 fixation and oil production through microalga. Energy Convers Manag 36(6–9):729–731

    Article  CAS  Google Scholar 

  2. Miao X, Wu Q (2006) Biodiesel production from heterotrophic microalgal oil. Bioresour Technol 97(6):841–846

    Article  CAS  Google Scholar 

  3. Fredriksson S, Elwinger K, Pickova J (2006) Fatty acid and carotenoid composition of egg yolk as an effect of microalgae addition to feed formula for laying hens. Food Chem 99(3):530–537

    Article  CAS  Google Scholar 

  4. Scott DD, Srirama K, Carani BS (2007) Omega-3 fatty acids for nutrition and medicine: considering microalgae oil as a vegetarian source of EPA and DHA. Curr Diabetes Rev 3(3):198–203

    Article  Google Scholar 

  5. Harun R, Singh M, Forde GM, Danquah MK (2010) Bioprocess engineering of microalgae to produce a variety of consumer products. Renew Sustain Energy Rev 14(3):1037–1047

    Article  CAS  Google Scholar 

  6. Lloyd D, Turner G (1968) The cell wall of Prototheca zopfii. J Gen Microbiol 50(3):421–427

    Article  CAS  Google Scholar 

  7. Stanisavljevic IT, Lazic ML, Veljkovic VB (2007) Ultrasonic extraction of oil from tobacco (Nicotiana tabacum L.) seeds. Ultrason Sonochem 14(5):646–652

    Article  CAS  Google Scholar 

  8. Cravotto G, Boffa L, Mantegna S, Perego P, Avogadro M, Cintas P (2008) Improved extraction of vegetable oils under high-intensity ultrasound and/or microwaves. Ultrason Sonochem 15(5):898–902

    Article  CAS  Google Scholar 

  9. Uquiche E, Jeréz M, Ortíz J (2008) Effect of pretreatment with microwaves on mechanical extraction yield and quality of vegetable oil from Chilean hazelnuts (Gevuina avellana Mol). Innov Food Sci Emerg Tech 9(4):495–500

    Article  CAS  Google Scholar 

  10. Chisti Y, Moo-Young M (1986) Disruption of microbial cells for intracellular products. Enzym Microb Tech 8(4):194–204

    Article  CAS  Google Scholar 

  11. Adam F, Abert-Vian M, Peltier G, Chemat F (2012) “Solvent-free” ultrasound-assisted extraction of lipids from fresh microalgae cells: a green, clean and scalable process. Bioresour Technol 114:457–465

    Article  CAS  Google Scholar 

  12. Ganzler K, Szinai I, Salgó A (1990) Effective sample preparation method for extracting biologically active compounds from different matrices by a microwave technique. J Chromatogr A 520:257–262

    Article  CAS  Google Scholar 

  13. Jassie L, Revesz R, Kierstead T, Hasty E, Matz S (1997) Microwave-assisted solvent extraction. In: Kingston HMS, Haswell SJ (eds) Microwave enhanced chemical society: fundamentals, sample preparation, and applications. American Chemical Society, Washington DC

    Google Scholar 

  14. Lee JY, Yoo C, Jun SY, Ahn CY, Oh HM (2010) Comparison of several methods for effective lipid extraction from microalgae. Bioresour Technol 101(1):S75–S77

    Article  CAS  Google Scholar 

  15. Wen YH, Su JC, Ma YA, Chen JM, Yu CC (2010) Study and development of a reactor system with the double helical ribbons impeller for algae (in Chinese). J Agric For 59(3):253–271

    Google Scholar 

  16. Guillard RL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH (eds) Culture of marine invertebrate animals, 1st edn. Plenum Publishing Corporation, New York

    Google Scholar 

  17. Folch J, Lees M, Sloane SGH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226(1):497–509

    CAS  Google Scholar 

  18. AOCS (1999) Official methods and recommended practices of the American Oil Chemists’ Society, 5th edn. American Oil Chemists’ Society, Champaign

    Google Scholar 

  19. Morrison WR, Smith LM (1964) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J Lipid Res 5:600–608

    CAS  Google Scholar 

  20. Vanhille C, Campos-Pozuelo C (2012) Acoustic cavitation mechanism: a nonlinear model. Ultrason Sonochem 19(2):217–220

    Article  CAS  Google Scholar 

  21. Dejoye C, Vian MA, Lumia G, Bouscarle C, Charton F, Chemat F (2011) Combined extraction processes of lipid from Chlorella vulgaris microalgae: microwave prior to supercritical carbon dioxide extraction. Int J Mol Sci 12(12):9332–9341

    Article  CAS  Google Scholar 

  22. Shimizu N, Ogino C, Dadjour MF, Ninomiya K, Fujihira A, Sakiyama K (2008) Sonocatalytic facilitation of hydroxyl radical generation in the presence of TiO2. Ultrason Sonochem 15(6):988–994

    Article  CAS  Google Scholar 

  23. Sonnta CV (2008) Advanced oxidation processes: mechanistic aspects. Water Sci Technol 58(5):1015–1021

    Article  Google Scholar 

  24. Liljeblad JFD, Bulone V, Tyrode E, Rutland MW, Johnson CM (2010) Phospholipid monolayers probed by vibrational sum frequency spectroscopy: instability of unsaturated phospholipids. Biophys J 98(10):L50–L52

    Article  CAS  Google Scholar 

  25. Lü JM, Lin PH, Yao Q, Chen C (2010) Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems. J Cell Mol Med 14(4):840–860

    Article  Google Scholar 

  26. Pingret D, Fabiano-Tixier A-S, Chemat F (2013) Degradation during application of ultrasound in food processing: a review. Food Control 31(2):593–606

    Article  Google Scholar 

  27. Pingret D, Durand G, Fabiano-Tixier A-S, Rockenbauer A, Ginies C, Chemat F (2012) Degradation of edible oil during food processing by ultrasound: electron paramagnetic resonance, physicochemical, and sensory appreciation. J Agric Food Chem 60(31):7761–7768

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank the National Science Council of Taiwan for financial support under grant NSC-99-2313-B-005-014. We are grateful to Dr. Chung Wen-Hsin of the Department of Plant Pathology at the National Chung Hsing University of Taiwan for help in operating the SEM, and to Dr. Chung Wen-Chuan of the Taiwan Seed Improvement and Propagation Station, COA, for help with improving this article.

Author information

Authors and Affiliations

  1. Department of Bio-Industrial Mechatronics Engineering, National Chung Hsing University, Taichung, 402, Taiwan

    Yu-An Ma, Yi-Ming Cheng & Chung-Chyi Yu

  2. Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan

    Jenn-Wen Huang

  3. Department of Chemistry, National Chung Hsing University, Taichung, 402, Taiwan

    Jen-Fon Jen

  4. Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, 402, Taiwan

    Yung-Sheng Huang

  5. Department of Nutrition, I-Shou University, Kaohsiung, 840, Taiwan

    Yung-Sheng Huang

Authors
  1. Yu-An Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yi-Ming Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jenn-Wen Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jen-Fon Jen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yung-Sheng Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chung-Chyi Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chung-Chyi Yu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ma, YA., Cheng, YM., Huang, JW. et al. Effects of ultrasonic and microwave pretreatments on lipid extraction of microalgae. Bioprocess Biosyst Eng 37, 1543–1549 (2014). https://doi.org/10.1007/s00449-014-1126-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-014-1126-4

Keywords

Search

Navigation