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Optimization and production of pyrrolidone antimicrobial agent from marine sponge-associated Streptomyces sp. MAPS15

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Abstract

Twenty-nine actinobacterial strains were isolated from marine sponge Spongia officinalis and screened for antagonistic activity against various bacterial and fungal pathogens. The active antibiotic producer MAPS15 was identified as Streptomyces sp. using 16S rRNA phylogenetic analysis. The critical control factors were selected from Plackett–Burman (PB) factorial design and the bioprocess medium was optimized by central composite design (CCD) for the production of bioactive metabolite from Streptomyces sp. MAPS15. The maximum biomass and active compound production obtained with optimized medium was 6.13 g/L and 62.41 mg/L, respectively. The economical carbon source, paddy straw was applied for the enhanced production of bioactive compound. The purified active fraction was characterized and predicted as pyrrolidone derivative which showed broad spectrum of bioactivity towards indicator organisms. The predicted antimicrobial spectra suggested that the Streptomyces sp. MAPS15 can produce a suite of novel antimicrobial drugs.

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Acknowledgments

Authors thank Council of Scientific and Industrial Research (CSIR), New Delhi, India for research grant.

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Authors and Affiliations

  1. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India

    G. Sathiyanarayanan, R. Gandhimathi & B. Sabarathnam

  2. Department of Food Science and Technology, Pondicherry University, R.V. Nagar, Kalapet, Puducherry, 605 014, India

    G. Seghal Kiran

  3. Centre for Microbiology, Pondicherry University, R.V. Nagar, Kalapet, Puducherry, 605 014, India

    Joseph Selvin

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  1. G. Sathiyanarayanan
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  2. R. Gandhimathi
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  3. B. Sabarathnam
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  4. G. Seghal Kiran
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  5. Joseph Selvin
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Correspondence to Joseph Selvin.

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Sathiyanarayanan, G., Gandhimathi, R., Sabarathnam, B. et al. Optimization and production of pyrrolidone antimicrobial agent from marine sponge-associated Streptomyces sp. MAPS15. Bioprocess Biosyst Eng 37, 561–573 (2014). https://doi.org/10.1007/s00449-013-1023-2

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  • DOI: https://doi.org/10.1007/s00449-013-1023-2

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