Abstract
Agar-supported fermentation (Ag-SF), a variant of solid-state fermentation, has recently been improved by the development of a dedicated 2 m2 scale pilot facility, Platotex. We investigated the application of solid-phase extraction (SPE) to Ag-SF in order to increase yields and minimize the contamination of the extracts with agar constituents. The selection of the appropriate resin was conducted on liquid-state fermentation and Diaion HP-20 exhibited the highest recovery yield and selectivity for the metabolites of the model fungal strains Phomopsis sp. and Fusarium sp. SPE applied to Ag-SF resulted in a particular compartmentalization of the culture. The mycelium that requires oxygen to grow migrates to the top layer and formed a thick biofilm. The resin beads intercalate between the agar surface and the mycelium layer, and trap directly the compounds secreted by the mycelium through a “solid–solid extraction” (SSE) process. The resin/mycelium layer is easily recovered by scraping the surface and the target metabolites extracted by methanol. Ag-SF associated to SSE represents an ideal compromise for the production of bioactive secondary metabolites with limited economic and environmental impact.
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Acknowledgments
The authors are grateful to Dr. Boonsom Bussaban and Pr. Saisamorn Lumyong, from Chiang Mai University, Thailand, for providing the fungal strains. This research was supported by a scholarship grant from the Institut de Chimie des Substances Naturelles, ICSN-CNRS.
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Le Goff, G., Adelin, E., Cortial, S. et al. Application of solid-phase extraction to agar-supported fermentation. Bioprocess Biosyst Eng 36, 1285–1290 (2013). https://doi.org/10.1007/s00449-012-0873-3
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DOI: https://doi.org/10.1007/s00449-012-0873-3