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Carboxymethylcellulose–gelatin–superoxidase dismutase electrode for amperometric superoxide radical sensing

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Abstract

A novel, highly sensitive superoxide dismutase biosensor for the direct and simultaneous determination of superoxide radicals was developed by immobilization of superoxide dismutase within carboxymethylcellulose–gelatin on a Pt electrode surface. The parameters affecting the performance of the biosensor were investigated. The response of the CMC–G–SOD biosensor was proportional to O ·−2 concentration and the detection limit was 1.25 × 10−3 mM with a correlation coefficient of 0.9994. The developed biosensor exhibited high analytical performance with wider linear range, high sensitivity and low response time. The biosensor retained 89.8% of its sensitivity after use for 80 days. The support system enhanced the immobilization of superoxide dismutase and promoted the electron transfer of superoxide dismutase minimizing its fouling effect. The biosensor was quite effective not only in detecting O ·−2 , but also in determining the antioxidant properties of acetylsalicylic acid-based drugs and the anti-radical activity of healthy and cancerous human brain tissues.

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Acknowledgments

This work financially supported by the Scientific and Technological Research Council of Turkey (no. 108T131).

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Authors and Affiliations

  1. Biotechnology Section, Department of Biology, Science Faculty, Ankara University, Tandoğan, Ankara, 06100, Turkey

    Sümer Aras

  2. Department of Chemistry, Science Faculty, Ankara University, Tandoğan, Ankara, 06100, Turkey

    Ozge Kocabay, Emel Emregul & Kaan Cebesoy Emregul

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  1. Ozge Kocabay
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  2. Emel Emregul
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  3. Sümer Aras
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  4. Kaan Cebesoy Emregul
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Correspondence to Emel Emregul.

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Kocabay, O., Emregul, E., Aras, S. et al. Carboxymethylcellulose–gelatin–superoxidase dismutase electrode for amperometric superoxide radical sensing. Bioprocess Biosyst Eng 35, 923–930 (2012). https://doi.org/10.1007/s00449-011-0677-x

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  • DOI: https://doi.org/10.1007/s00449-011-0677-x

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