Abstract
To probe the effects of renewal regime on the production of polysaccharides, Porphyridium cruentum was cultured semi-continuously in flat plate photobioreactor. Uniform design was used to optimize renewal conditions. Quadratic mathematic models related to productivity, total recovery yield of biomass and polysaccharides were set up to clarify the influence of individual factors and their interactions. According to the mathematic models, the optimal semi-continuous condition for total yield of polysaccharide was NaNO3 3.5 g/L, renewal rate 27%, renewal period 2.91 days. The optimal condition for polysaccharide output rate was NaNO3 0.5 g/L, renewal rate 5%, renewal period 7 days. With the optimal renewal regime, the maximal total recovery yields of polysaccharide achieved at 29.4 g, which was 1.57 times higher than that of batch cultivation. The maximum output rate of polysaccharide was 68.64 mg/L per day, which was 2.02 times higher than previous reported data.
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We are thankful to the Natural Science-Foundation of Province Shandong, China (No. Y2007D59) for the financial support.
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Sun, L., Wang, C., Ma, C. et al. Optimization of renewal regime for improvement of polysaccharides production from Porphyridium cruentum by uniform design. Bioprocess Biosyst Eng 33, 309–315 (2010). https://doi.org/10.1007/s00449-009-0325-x
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DOI: https://doi.org/10.1007/s00449-009-0325-x