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An effective and simplified pH-stat control strategy for the industrial fermentation of vitamin B12 by Pseudomonas denitrificans

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Abstract

In order to improve the productivity of vitamin B12 by Pseudomonas denitrificans carried out in a 120-m3 fermenter, the effect of pH on vitamin B12 biosynthesis was investigated. Results obtained from shake flask experiments showed that the feeding of carbon source (beet molasses or glucose) and methyl-group donor (betaine or choline chloride) significantly influenced the pH and the biosynthesis of vitamin B12. In contrast to beet molasses or choline chloride, using glucose as a feed medium and betaine as a methyl-group donor, pH could be maintained at a stable range. As a result, higher vitamin B12 production was achieved. Accordingly, an effective and simplified pH-stat control strategy was established for the fermentation of vitamin B12 in a 120-m3 industrial fermenter. When the new pH control strategy was applied, pH was stably kept in the range of 7.15–7.30 during fermentation. Thus, 214.3 μg/mL of vitamin B12 was achieved.

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Acknowledgments

This work was supported by National Basic Research Program (973 Program, No. 2007CB714303) and Shanghai Leading Academic Discipline Project (B505). We are grateful to Shijiazhuang Pharma. Group Hua Rong Pharmaceutical Co. Ltd for their support in this work.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Kun-Tai Li, Dong-Hong Liu, Ju Chu, Yong-Hong Wang, Ying-Ping Zhuang & Si-Liang Zhang

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  1. Kun-Tai Li
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  2. Dong-Hong Liu
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  3. Ju Chu
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  4. Yong-Hong Wang
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Correspondence to Si-Liang Zhang.

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Li, KT., Liu, DH., Chu, J. et al. An effective and simplified pH-stat control strategy for the industrial fermentation of vitamin B12 by Pseudomonas denitrificans . Bioprocess Biosyst Eng 31, 605–610 (2008). https://doi.org/10.1007/s00449-008-0209-5

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  • DOI: https://doi.org/10.1007/s00449-008-0209-5

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