Abstract
Mesenchymal stem cells (MSCs) are used extensively in cell therapy for repair and regeneration of several organs and tissues. Cell therapy is a valuable option to treat neurodegenerative diseases and MSCs have been shown to improve neuronal function through direct differentiation or secretion of neurotrophic factors. In the present study, we isolated and characterized stem cells from medial and central orbital adipose tissue and found that they could be grown in a monolayer culture. The orbital adipose tissue-derived cells were identical to bone marrow-derived MSCs in their cell surface marker expression, gene expression and multilineage differentiation abilities. The orbital adipose-derived MSCs (OAMSCs) express several neurotrophic factors, possess neuroectodermal differentiation ability and secreted factors from OAMSCs abrogated neuronal cell damage induced by oxidative stress. Thus, OAMSCs might be a valuable cell source for treatment of neurological diseases and to reverse oxidative damage in the neuronal cells.
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Acknowledgments
DM, AS, RS and AK were supported by MHRD, Govt. of India.
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This work was supported by funds from the Indian Institute of Technology Guwahati (IITG).
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KB and BGJ conceived the study. DM, AK and BGJ designed the study. KB performed blepharoplasty surgery. DM, AS, RS and AK performed experiments. DM, KB, JB, HB, ND and BGJ analyzed the data. DM, KB and BGJ wrote the manuscript. All the authors approved the final version of the manuscript.
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All procedures involving human samples were in accordance with the Helsinki convention and approved by the ethics committee of the Indian Institute of Technology Guwahati and Sri Sankaradeva Nethralaya, Guwahati. The study was carried out in accordance with the human ethics committee guidelines and written informed consent was obtained from all the patients involved in the study.
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Mawrie, D., Bhattacharjee, K., Sharma, A. et al. Human orbital adipose tissue-derived mesenchymal stem cells possess neuroectodermal differentiation and repair ability. Cell Tissue Res 378, 531–542 (2019). https://doi.org/10.1007/s00441-019-03072-0
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DOI: https://doi.org/10.1007/s00441-019-03072-0