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Distribution of voltage-dependent and intracellular Ca2+ channels in submucosal neurons from rat distal colon

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Abstract

We recently observed a bradykinin-induced increase in the cytosolic Ca2+ concentration in submucosal neurons of rat colon, an increase inhibited by blockers of voltage-dependent Ca2+ (Cav) channels. As the types of Cav channels used by this part of the enteric nervous system are unknown, the expression of various Cav subunits has been investigated in whole-mount submucosal preparations by immunohistochemistry. Submucosal neurons, identified by a neuronal marker (microtubule-associated protein 2), are immunoreactive for Cav1.2, Cav1.3 and Cav2.2, expression being confirmed by reverse transcription plus the polymerase chain reaction. These data agree with previous observations that the inhibition of L- and N-type Ca2+ currents strongly inhibits the response to bradykinin. However, whole-cell patch-clamp experiments have revealed that bradykinin does not enhance Ca2+ inward currents under voltage-clamp conditions. Consequently, bradykinin does not directly interact with Cav channels. Instead, the kinin-induced Ca2+ influx is caused indirectly by the membrane depolarization evoked by this peptide. As intracellular Ca2+ channels on Ca2+-storing organelles can also contribute to Ca2+ signaling, their expression has been investigated by imaging experiments and immunohistochemistry. Inositol 1,4,5-trisphosphate (IP3) receptors (IP3R) have been functionally demonstrated in submucosal neurons loaded with the Ca2+-sensitive fluorescent dye, fura-2. Histamine, a typical agonist coupled to the phospholipase C pathway, induces an increase in the fura-2 signal ratio, which is suppressed by 2-aminophenylborate, a blocker of IP3 receptors. The expression of IP3R1 has been confirmed by immunohistochemistry. In contrast, ryanodine, tested over a wide concentration range, evokes no increase in the cytosolic Ca2+ concentration nor is there immunohistochemical evidence for the expression of ryanodine receptors in these neurons. Thus, rat submucosal neurons are equipped with various types of high-voltage activated Cav channels and with IP3 receptors for intracellular Ca2+ signaling.

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Authors and Affiliations

  1. Institute for Veterinary Physiology and Biochemistry, Justus-Liebig-University, Giessen, Germany

    Matthias Rehn, Sandra Bader, Anna Bell & Martin Diener

  2. Institut für Veterinär-Physiologie und -Biochemie, Universität Gießen, Frankfurter Strasse 100, Gießen, Germany

    Martin Diener

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  1. Matthias Rehn
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  2. Sandra Bader
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  3. Anna Bell
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  4. Martin Diener
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Correspondence to Martin Diener.

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This work was supported by the Deutsche Forschungsgemeinschaft (grant Di 388/13-1).

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Rehn, M., Bader, S., Bell, A. et al. Distribution of voltage-dependent and intracellular Ca2+ channels in submucosal neurons from rat distal colon. Cell Tissue Res 353, 355–366 (2013). https://doi.org/10.1007/s00441-013-1643-5

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