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Freezing solution containing dimethylsulfoxide and fetal calf serum maintains survival and ultrastructure of goat preantral follicles after cryopreservation and in vitro culture of ovarian tissue

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Abstract

Goat ovarian cortex fragments were subjected to slow freezing in the presence of various solutions containing intracellular cryoprotectants, including 1.0 M ethylene glycol (EG), propanediol (PROH), or dimethyl sulfoxide (DMSO), with or without sucrose and/or fetal calf serum (FCS). Histological examination revealed that only the DMSO-containing solutions were able to maintain a follicular ultrastructure similar to the morphology observed in the fresh control. Therefore, fragments previously cryopreserved in DMSO solutions (with and without sucrose and/or FCS) were cultured in vitro for 48 h and then subjected to viability, histological, and ultrastructural analysis. No significant differences were observed among the percentages of morphologically normal follicles in cryopreserved ovarian tissue before in vitro culture (DMSO: 62.5%; DMSO + sucrose: 68.3%; DMSO + FCS: 60.0%; DMSO + sucrose + FCS: 60.0%) and after culture (DMSO: 60.8%; DMSO + sucrose: 64.2%; DMSO + FCS: 70.8%; DMSO + sucrose + FCS: 55.0%). Following in vitro culture, the viability analysis showed that only the freezing solution containing DMSO and FCS (75.6%) maintained a percentage of viable follicles similar to that observed after culture without cryopreservation (89.3%). As determined by ultrastructural analysis, morphologically normal preantral follicles were detected in the fresh control and in fragments cultured before and after cryopreservation with DMSO and FCS. Thus, a freezing solution containing DMSO and FCS, under the experimental conditions tested here, guaranteed the maintenance of viability and follicular ultrastructure after short-term in vitro culture.

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Authors and Affiliations

  1. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil

    Simone Vieira Castro, Adeline Andrade de Carvalho, Cleidson Manoel Gomes da Silva, Luciana Rocha Faustino, Cláudio Cabral Campello, José Ricardo de Figueiredo & Ana Paula Ribeiro Rodrigues

  2. Laboratory of Microscopy, Department of Cell Biology, University of Brasilia, Brasilia, DF, Brazil

    Carolina Madeira Lucci & Sônia Nair Báo

  3. Faculdade de Medicina Veterinária, Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, Fortaleza, CE, Brazil, CEP: 60740-000

    Simone Vieira Castro

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  1. Simone Vieira Castro
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  2. Adeline Andrade de Carvalho
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  3. Cleidson Manoel Gomes da Silva
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  4. Luciana Rocha Faustino
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  8. José Ricardo de Figueiredo
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  9. Ana Paula Ribeiro Rodrigues
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Correspondence to Simone Vieira Castro.

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Castro, S.V., de Carvalho, A.A., da Silva, C.M.G. et al. Freezing solution containing dimethylsulfoxide and fetal calf serum maintains survival and ultrastructure of goat preantral follicles after cryopreservation and in vitro culture of ovarian tissue. Cell Tissue Res 346, 283–292 (2011). https://doi.org/10.1007/s00441-011-1257-8

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  • DOI: https://doi.org/10.1007/s00441-011-1257-8

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