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Multi-potent progenitors in freshly isolated and cultured human mesenchymal stem cells: a comparison between adipose and dermal tissue

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Abstract

Mesenchymal stem cells (MSCs) from human adult adipose tissue (A-MSCs) have a better differentiative ability than MSCs derived from the derma (D-MSCs). To test whether this difference is associated with differences in the content of multi-potent progenitors in A-MSCs, the number and the differentiative properties of multi-potent progenitors have been analyzed in various preparations of A-MSCs and D-MSCs. Adipogenic and osteogenic differentiation performed on colony-forming units have revealed that adipogenic and osteogenic progenitors are similar in the two populations, with only a slighty better performance of A-MSCs over D-MSCs from passages p0 to p15. An analysis of the presence of tri-, bi-, uni- and nulli-potent progenitors isolated immediately after isolation from tissues (p0) has shown comparable numbers of tri-potent and bi-potent progenitors in MSCs from the two tissues, whereas a higher content in uni-potent cells committed to adipocytes and a lower content in nulli-potent cells has been observed in A-MSCs. Furthermore, we have characterized the progenitors present in A-MSCs after six passages in vitro to verify the way in which in vitro culture can affect content in progenitor cells. We have observed that the percentage of tri-potent cells in A-MSCs at p6 remains similar to that observed at p0, although bi-potent and uni-potent progenitors committed to osteogenic differentiation increase at p6, whereas nulli-potent cells decrease at p6. These data indicate that the greater differentiative ability of A-MSC populations does not correlate directly with the number of multi-potent progenitors, suggesting that other factors influence the differentiation of bulk populations of A-MSCs.

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Authors and Affiliations

  1. Molecular Medicine Section, Department of Neuroscience, and Center for Stem Cell Research, University of Siena, Via Aldo Moro, 53100, Siena, Italy

    Ivana Manini, Letizia Gulino, Barbara Gava, Enrico Pierantozzi, Carlo Curina, Daniela Rossi & Vincenzo Sorrentino

  2. Plastic and Reconstructive Surgery Unit, University of Siena, Siena, Italy

    Anna Brafa & Carlo D’Aniello

Authors
  1. Ivana Manini
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  2. Letizia Gulino
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  3. Barbara Gava
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  4. Enrico Pierantozzi
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  5. Carlo Curina
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  6. Daniela Rossi
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  7. Anna Brafa
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  8. Carlo D’Aniello
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  9. Vincenzo Sorrentino
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Corresponding author

Correspondence to Vincenzo Sorrentino.

Additional information

Ivana Manini and Letizia Gulino contributed equally to this work.

This work was supported by grants from the Regione Toscana and from the Fondazione Monte dei Paschi di Siena.

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Supplemental Table 1

Cell surface antigen profile of bone-marrow-derived mesenchymal stem cells (BM-MSC), adipose-tissue-derived MSC (A-MSC) and dermal-derived MSC (D-MSC) cell populations. Analysis by fluorescence-activated cell sorting was performed on three independent populations for each tissue between passages 3 and 5. Data are expressed as the percentage mean of positive cells ± SEM within MSC cultures (DOC 34 kb)

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Manini, I., Gulino, L., Gava, B. et al. Multi-potent progenitors in freshly isolated and cultured human mesenchymal stem cells: a comparison between adipose and dermal tissue. Cell Tissue Res 344, 85–95 (2011). https://doi.org/10.1007/s00441-011-1139-0

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  • DOI: https://doi.org/10.1007/s00441-011-1139-0

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