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Cloning and expression pattern of a putative octopamine/tyramine receptor in antennae of the noctuid moth Mamestra brassicae

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Abstract

In insects, biogenic amines have been shown to play an important role in olfactory plasticity. In a first attempt to decipher the underlying molecular mechanisms, we report the molecular cloning and precise expression pattern of a newly identified octopamine/tyramine-receptor-encoding gene in the antennae of the noctuid moth Mamestra brassicae (MbraOAR/TAR). A full-length cDNA has been obtained through homology cloning in combination with rapid amplification of cDNA ends/polymerase chain reaction; the deduced protein exhibits high identities with previously identified octopamine/tyramine receptors in other moths. In situ hybridization within the antennae has revealed that MbraOAR/TAR is expressed at the bases of both pheromone-sensitive and non-sensitive olfactory sensilla and in cells with a neurone-like shape. In accordance with previous physiological studies that have revealed a role of biogenic amines in the electrical activity of the receptor neurones, our results suggest that biogenic amines (either octopamine or tyramine) target olfactory receptor neurones to modulate olfactory coding as early as the antennal level.

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Acknowledgments

The authors thank Sylvia Anton and Philippe Lucas (UMR PISC, INRA Versailles, France) for valuable discussions and support.

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Author notes

  1. Xavier Grosmaître

    Present address: Department of Neuroscience, University of Pennsylvania, 109 Johnson Pavilion, 3600 Hamilton Walk, Philadelphia, PA 19104, USA

Authors and Affiliations

  1. INRA, UMR 1272 INRA-UPMC-AgroParisTech PISC Physiologie de l’Insecte: Signalisation et Communication, Route de Saint-Cyr, 78000, Versailles, France

    Isabelle Brigaud, Marie-Christine François & Emmanuelle Jacquin-Joly

Authors
  1. Isabelle Brigaud
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  2. Xavier Grosmaître
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  3. Marie-Christine François
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  4. Emmanuelle Jacquin-Joly
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Corresponding author

Correspondence to Emmanuelle Jacquin-Joly.

Additional information

This work was supported by INRA, Université Paris VI, ACI JC5249 funding and by an ACI JC doctoral fellowship to Isabelle Brigaud.

Dr. Isabelle Brigaud and Dr. Xavier Grosmaître has contributed equally to the work.

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Brigaud, I., Grosmaître, X., François, MC. et al. Cloning and expression pattern of a putative octopamine/tyramine receptor in antennae of the noctuid moth Mamestra brassicae . Cell Tissue Res 335, 455–463 (2009). https://doi.org/10.1007/s00441-008-0722-5

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  • DOI: https://doi.org/10.1007/s00441-008-0722-5

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